1,721,074 research outputs found
Growth pattern of a transplantable acute myeloid leukemia in the rat
No full textA generalized acute myeloid leukemia was induced in inbred Sprague-Dawley rats by intravenous injection of chloroleukemia cells. In the bone marrow, colonization by blast cells was observed soon after transplantation and at the end of the disease leukemia cells accounted for about 70% of the whole cell population. In the peripheral blood, erythrocyte and platelet counts decreased late in the disease, while from day 5 onwards there was a progressive increase in the leukocyte count owing to blast cells released into the bloodstream. Spleen, liver and central nervous system were also involved in the disease. The survival time of transplanted animals correlated with the number of cells injected: after transplantation of 2 × 107 chloroleukemia cells survival time was 8.3 days, and with each 10-fold reduction of the inoculum survival time increased by about 2.5 days. The data presented show that Shay chloroleukemia mimics some features of human acute myeloid leukemia and possesses a predictable and highly reproducible growth rate, thus providing a further useful experimental model
ERYTHROPOIETIN - CLINICAL-APPLICATIONS
No full textThe last few years have seen an enormous increase in our knowledge on the haematopoietic growth factor erythropoietin (Epo), firstly with its purification and determination of its primary amino acid sequence, and more recently with the isolation of the Epo gene and its expression in mammalian cell lines. This review article summarizes the crucial biological features of Epo and critically examines the main results obtained in clinical trials on humans
RENAL-CANCER AFTER BUSULFAN TREATMENT FOR CHRONIC MYELOID-LEUKEMIA - THERAPEUTIC IMPLICATIONS
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Detailed kinetic analysis of Shay chloroleukaemia cell population propagated in permanent suspension culture in vitro
No full textDetailed kinetic analysis of a growing cell population is difficult, even when assay conditions are nearly ideal. Therefore, it is usually essential to perform several types of experiments and analyse all the results in terms of a mathematical model, the use of which is not limited a priori by a specified application. In the present study we investigated cell population kinetics using rat chloroleukaemia cells propagated in suspension culture in vitro. The parameters were measured: doubling time of the population, fraction of labelled mitoses, changes in labelling index with time after pulse labelling, continuous labelling and stathmokinetic index. Analysis of the results was based on a computer program CECAM, which is a stochastic model capable of simulating essentially all types of kinetic experiments based on presently known assay techniques. The results showed that precise and reliable information on cell population kinetics could not be obtained from the analysis of any single type of experimental data. In particular, the technique of labelled mitoses underestimated the duration of the G1 phase, owing to subtle label-induced changes in population behaviour. The changes could not have been detected with any certainty without rigorous quantitative comparisons with other types of experimental data. As a whole, however, results obtained by the different techniques were in agreement and the kinetic behaviour of chloroleukaemia cells in vitro could be established in detail. In certain circumstances even minute changes in the kinetic parameters of the cells can modify population behaviour drastically. To study these cases adequately the experiments must be designed with utmost care, preferably with the aid of preceding simulations. This is because demonstration of small primary changes in population kinetics may be beyond the limit of detection of any single assay method
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