155 research outputs found
Goede GGZ! Nieuwe concepten, aangepaste taal en betere organisatie
No full textPhilippe Delespaul, Micheal Milo, Frank Schalken, Wilma Boevink & Jim van Os. Goede GGZ! Nieuwe concepten, aangepaste taal en betere organisatie. Leusden: Diagnosis Uitgevers, 2016, 368 p., €35. ISBN 978949196911
B2B Customer Insight Tool: Automated Data Analytics to improve the Deal Analytics workflow
No full textThe Deal Analytics group of PricewaterhouseCoopers Amsterdam has requested a tool for automatising the business-to-business customer analysis. This analysis was performed manually, which left room for performance improvement. This report discusses how the a product was developed which automates the analyses After two weeks of initial research, a complete system was designed and implemented in the subsequent nine weeks. The tool consists of two distinct parts: a front-end and a back-end. The front-end allows the user to customise the analysis to its own preferences, and communicates with the back-end to efficiently perform the analysis. With the help of user evaluations, the front-end has been designed such that it is usable by any PwC employee within the Deals branch.The back-end uses data analysis techniques and machine learning to analyse customer behaviour. Strong points and growth opportunities of a company are found using techniques such as customer segmentation, regression analysis, and cross-sell analysis. The product has been tested using a variety of techniques to ensure that the software does not crash on unexpected input. The final product is evaluated based on the requirements, design goals and success criteria set at the start of the project and can be considered successful.Bachelor End ProjectBachelor Graduation ProjectBachelor ProjectBachelorprojectComputer Scienc
Pipavav harbour: Sedimenttransporten
No full textIn het noordwesten van India (zie atb 1) ligt de deelstaat Gujarat. In de tijd van de Britse overheersing in India behoorde Gujarat tot het zogenaamde 'native India'. Het gebied bestond uit 30 a 40 staatjes met ieder een eigen bestuur. De deelstaat Gujarat beschikt over weinig havens die voldoende diep zijn voor zeeschepen. De havens waar momenteel terminals voor zeeschepen zijn, zijn de havens van Kandia, Okha en Bhavnagar (zie atb 2). De haven van Kandia in het noorden van Gujarat beschikt over een kade die door zeegaande schepen kan worden aangedaan. Ook de havens van Okha en Bhavnagar hebben kaden voor het behandelen van grote schepen maar hebben beide een restrictie voor de diepgang van de schepen en kampen met grote verzandingsproblemen. Zeeschepen die de andere havens van Gujarat aandoen worden via lichters geladen of gelost. Met kleine vaartuigen (lichters) worden de goederen uit de ondiepe haven naar dieper water vervoerd waar de diepstekende schepen kunnen worden beladen. De wachttijden voor deze schepen kan hierbij oplopen tot 15 dagen. Deze tekortkoming van de bestaande havens hebben aanleiding gegeven voor een aantal ondernemers en de overheid van Gujarat verenigd in de GPPL (Gujarat Pipavav Port Limited) om een diepzeehaven te ontwikkelen bij Pipavav. De verzandingsproblemen zijn maatgevend voor dit afstudeeronderzoek.Havens en scheepvaartwegenHydraulic EngineeringCivil Engineering and Geoscience
An RxLR effector from phytophthora infestans prevents re-localisation of two plant NAC transcription factors from the endoplasmic reticulum to the nucleus
No full textThe plant immune system is activated following the perception of exposed, essential and invariant microbial molecules that are recognised as non-self. A major component of plant immunity is the transcriptional induction of genes involved in a wide array of defence responses. In turn, adapted pathogens deliver effector proteins that act either inside or outside plant cells to manipulate host processes, often through their direct action on plant protein targets. To date, few effectors have been shown to directly manipulate transcriptional regulators of plant defence. Moreover, little is known generally about the modes of action of effectors from filamentous (fungal and oomycete) plant pathogens. We describe an effector, called Pi03192, from the late blight pathogen Phytophthora infestans, which interacts with a pair of host transcription factors at the endoplasmic reticulum (ER) inside plant cells. We show that these transcription factors are released from the ER to enter the nucleus, following pathogen perception, and are important in restricting disease. Pi03192 prevents the plant transcription factors from accumulating in the host nucleus, revealing a novel means of enhancing host susceptibility
Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen <i>Phytophthora infestans</i>
No full textPlant pathogens establish infection by secretion of effector proteins that may be delivered inside host cells to manipulate innate immunity. It is increasingly apparent that the ubiquitin proteasome system (UPS) contributes significantly to the regulation of plant defences and, as such, is a target for pathogen effectors. Bacterial effectors delivered by the type III and IV secretion systems have been shown to interact with components of the host UPS. Some of these effectors possess functional domains that are conserved in UPS enzymes, whilst others contain novel domains with ubiquitination activities. Relatively little is known about effector activities in eukaryotic microbial plant pathogens. Nevertheless, effectors from oomycetes that contain an RXLR motif for translocation to the inside of plant cells have been shown to suppress host defences. Annotation of the genome of one such oomycete, the potato late blight pathogen Phytophthora infestans, and protein-protein interaction assays to discover host proteins targeted by the RXLR effector AVR3a, have revealed that this eukaryotic plant pathogen also has the potential to manipulate host plant UPS function
Sequence of Subterranean Clover Stunt Virus DNA: Affinities with the Geminiviruses
No full textAbstractThe nucleotide sequences of seven circular, single-stranded DNA components of one isolate of subterranean clover stunt virus (SCSV) have been determined. Each component, of about 1 kb, appears to encode a single open reading frame in the same sense as the encapsidated DNA. Notably, the proteins encoded by two SCSV components are related Each has a consensus nucleotide binding motif and shares about 40% amino acid identity with the other and with the putative replication proteins of banana bunchy top virus and coconut foliar decay virus. The noncoding regions of the five other SCSV components share a highly conserved noncoding sequence of about 160 nucleotides. All seven components were found to contain a sequence capable of forming a stable stem-loop structure in the noncoding region which contains a conserved 9-nucleotide sequence in the loop, very similar to that of the geminiviruses. In addition, the putative replication proteins of SCSV are similar to those of the geminiviruses. We suggest that the SCSV-like viruses and the geminiviruses share a common ancestor and that this ancestor was more like SCSV in particle structure and genome organisation
Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1
No full textFungal and oomycete plant pathogens translocate effector proteins into host cells to establish infection. However, virulence targets and modes of action of their effectors are unknown. Effector AVR3a from potato blight pathogen Phytophthora infestans is translocated into host cells and occurs in two forms: AVR3aKI, which is detected by potato resistance protein R3a, strongly suppresses infestin 1 (INF1)-triggered cell death (ICD), whereas AVR3aEM, which evades recognition by R3a, weakly suppresses host ICD. Here we show that AVR3a interacts with and stabilizes host U-box E3 ligase CMPG1, which is required for ICD. In contrast, AVR3aKI/Y147del, a mutant with a deleted C-terminal tyrosine residue that fails to suppress ICD, cannot interact with or stabilize CMPG1. CMPG1 is stabilized by the inhibitors MG132 and epoxomicin, indicating that it is degraded by the 26S proteasome. CMPG1 is degraded during ICD. However, it is stabilized by mutations in the U-box that prevent its E3 ligase activity. In stabilizing CMPG1, AVR3a thus modifies its normal activity. Remarkably, given the potential for hundreds of effector genes in the P. infestans genome, silencing Avr3a compromises P. infestans pathogenicity, suggesting that AVR3a is essential for virulence. Interestingly, Avr3a silencing can be complemented by in planta expression of Avr3aKI or Avr3aEM but not the Avr3aKI/Y147del mutant. Our data provide genetic evidence that AVR3a is an essential virulence factor that targets and stabilizes the plant E3 ligase CMPG1, potentially to prevent host cell death during the biotrophic phase of infection
Kwaliteitszorg in Delta scholen en in de Delta organisatie
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How to convert host plants into nonhosts
No full textRecent research demonstrates that undermining interactions between pathogen effectors and their host target proteins can reduce infection. As more effector–target pairs are identified, their structures and interaction surfaces exposed, and there is the possibility of making multiple edits to diverse plant genomes, the desire to convert crops to nonhosts could become reality.</p
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