436 research outputs found
In vivo whole cell mathematical model for metabolic reaction network for model organism Penicillium chrysogenum
No full textBiological systems present a complexity beyond intuitive comprehension and to obtain a better understanding of the behaviour of the living organisms, large scale dynamic mathematical models are employed. From a system biology perspective, these models should not only describe the kinetic behavior of metabolic reaction networks that feature metabolite-enzyme interactions (allosteric feedback or feed forward), intercompartmental transport, and cofactor coupling, but, they should also ultimately allow combining several pathways (horizontal modeling) and/or “omic” levels (vertical modeling) in the cell. However, currently by far most of the available models are limited to only one pathway and one “omic” level. In this work, a large scale kinetic metabolic model of Penicillium chrysogenum is developed, which aims to encompassing all the major pathways present in the organism. In constructing the kinetic model, we first define the stoichiometric network presented in van Gulik et al. (2000), which consists of 188 metabolites and 167 reactions located in 3 compartments (cytosol, mitochondria and peroxisome) and postulated a kinetic expression for each of the reactions. We used approximative linlog kinetics for the rate expressions, which allowed us to represent the enzyme-metabolite kinetic interactions by an elasticity matrix. Information on the presence and absence of mass action and allosteric enzyme kinetic information was obtained from literature survey and database search. The final values of the elasticities needed to be estimated by fitting the model to the available short term kinetic response data. Two major limitations have been encountered regarding the measurements of metabolites: (1) measurement of a metabolite at all and (2) compartmentation, i.e. measurements of metabolites/reactions that are present in multiple compartments (Nasution et al. 2006). To deal with the compartmentation problem, the system has been reduced 89 metabolites and 75 reactions within one compartment, by lumping, using insights gained both from biochemical knowledge and from data recently published by our group on short term kinetic responses of primary metabolism of Penicillium chrysogenum (Nasution et al. 2006). The limited number of available measurements was dealt by data-driven model reduction while applying the parameter estimation scheme to the large model. To estimate the kinetic parameters, the aim was to followed the methodology presented in Nikerel et al. (2006) in which the theory was applied to a small example system. However due to time constrains no kinetic parameter has been estimated.BioprocesstechnologyBiotechnologyApplied Science
Upwards along the pipes: Utilizing the recyclable potentials of local organic waste to revitalize the Cikapundung river area in Bandung, Indonesia
No full text“Upwards along the pipes” focuses on the Tamansari subdistrict in Bandung, Indonesia, in which the Cikapundung river is a central physical element, dividing the highly dense informal settlements in two. The project tries to tackle several ecological and spatial issues occuring within the site, including a large amount of (organic) waste being disposed in the river, decreasing the healthy living conditions of the site. The current situation lacks any form of decent centralised or decentralised wastewater treatment management and/ or infrastructure. Furthermore, there is a lack of public open spaces and functions within the kampungs, while the city in the future has to face an increasing population density, putting even more pressure on the city and especially these already dense kampungs. The project contains the development of a fast and easy applicable on-site wastewater treatment system which is able to directly prevent river pollution, while incrementally developing into a permanent decentralized wastewater treatment plant with an integrated public function, utilizing the potential beneficial values of recycled organic waste, in the forms of biogas and fertilizers. These output resources make environmental care economically more beneficial and appealing for local inhabitants. The integrated communal food market provides a platform for the kampung inhabitants to optimally benefit from the gained resources, by communally growing and selling food grown due to the gained fertilizers and cooked on the produced biogas from water treatment.Architecture, Urbanism and Building Science
Regulatie van de voedselinname en het ontstaan van hyperfagie tijdens de zwangerschap.
No full textDe begeleider en/of auteur heeft geen toestemming gegeven tot het openbaar maken van de scriptie.
The supervisor and/or the author did not authorize public publication of the thesis.
The nephrology crystal ball: The medium-term future
No full textIn January 2019, the ERA-EDTA surveyed nephrologists with questions on kidney care and kidney research designed to explore comprehension of the impact of alterations to organization of renal care and of advancements in technology and knowledge of kidney disease. Eight hundred and twenty-five ERA-EDTA members, ∼13% of the whole ERA-EDTA membership, replied to an ad hoc questionnaire. More than half of the respondents argued that kidney centres will be increasingly owned by large dialysis providers, nearly a quarter of respondents felt that many medical aspects of dialysis will be increasingly overseen by non-nephrologists and a quarter (24%) also believed that the care and long-term follow-up of kidney transplant patients will be increasingly under the responsibility of transplant physicians caring for patients with any organ transplant. Nearly half of the participants (45%, n = 367) use fully electronic clinical files integrating the clinical ward, the outpatient clinics, the haemodialysis and peritoneal dialysis units, as well as transplantation. Smartphone-based self-management programmes for the care of chronic kidney disease (CKD) patients are scarcely applied (only 11% of surveyed nephrologists), but a substantial proportion of respondents (74%) are eager to know more about the potential usefulness of these apps. Finally, European nephrologists expressed a cautious optimism about the application of omic sciences to nephrology and on wearable and implantable kidneys, but their expectations for the medium term are limited
Kerkvoogden in verzet: de Leersumsche quaestie
No full textContains fulltext :
146635.pdf (Publisher’s version ) (Open Access)XII, 227 p
Adviezen van reclasseringsmedewerkers aan rechters over de op te leggen strafsoort: Opvolgen of terzijde schuiven?
No full textRechters kunnen door een reclasseringsmedewerker worden geadviseerd over de op te leggen strafsoort. Op internationaal niveau is er een ‘uitbarsting’ geweest in onderzoek naar advies rapporten van de reclassering (Beyens & Scheirs, 2010). De overheersende zorg hierbij is geweest om te bepalen in welke mate reclasseringsrapporten een verschil maken bij de uitkomsten van strafoplegging. De wisselwerking tussen de rechtspraak en de reclassering bepaalt in hoge mate de kwaliteit van de Nederlandse rechtspleging.
Uit verschillende onderzoeken komt naar voren dat er een tegenstelling bestaat in de literatuur over de invloed van deze adviezen op de uiteindelijke keuze die rechters maken tussen verschillende strafsoorten. Enerzijds wordt verondersteld dat adviesrapporten van reclasseringsmedewerkers een geringe invloed hebben op de keuze van rechters tussen strafsoorten. Anderzijds wordt verondersteld dat adviesrapporten van reclasseringsmedewerkers een aanzienlijke invloed hebben op de keuze van rechters tussen strafsoorten. De vraag is of beide opvattingen over de invloed van adviesrapporten van de reclassering vertegenwoordigd zijn in de rechtbank
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