28 research outputs found
Assessment of cariogenic biofilms using drug encapsulated hydrogel membrane
No full textDental caries is a multifactorial disease primarily driven by cariogenic biofilms. Current antimicrobial treatments lack sustained release and localized action, leading to reduced efficacy and potential microbial resistance. Hydrogel-based drug delivery systems offer a promising alternative due to their biocompatibility, tunable properties, and ability to provide controlled antimicrobial release.
Objective: This study aimed to develop, characterize, and evaluate the efficacy of a chlorhexidine digluconate (CHX)-encapsulated chitosan-alginate hydrogel membrane against Streptococcus mutans and Lactobacillus acidophilus biofilms.Methods: Hydrogel membranes were synthesized via ionic cross-linking and characterized using Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), swelling studies, and drug release kinetics. Biofilms were grown on hydroxyapatite discs, and the antibacterial efficacy was assessed through zone of inhibition, Minimum Biofilm Inhibitory Concentration (MBIC), colony-forming unit (CFU) counts, and confocal laser scanning microscopy (CLSM). Biocompatibility was evaluated using an MTT assay on human gingival fibroblasts.Results: The hydrogel membrane exhibited a uniform porous structure with a high swelling ratio (350±25%) in simulated saliva and sustained CHX release over 72 hours. A significant reduction in biofilm viability (>90%) was observed compared to controls (p<0.001). CLSM images confirmed extensive biofilm disruption. The hydrogel showed no cytotoxicity to human gingival fibroblasts.
Conclusion: The CHX-encapsulated hydrogel membrane effectively inhibited cariogenic biofilms while maintaining biocompatibility, demonstrating its potential as a novel, sustained-release platform for caries prevention and management
Deriving magnetite nanostructures from natural resources and investigation of its erythrocyte compatibility
No full textHemocompatibility of Copper oxide with BSA for osteoblast application.
No full textThe improvement of the physical structure and biological activity of biomaterials is significantly influenced by copper oxide. As a trace element in the human body, copper (Cu) not only possesses antibacterial properties and a range of biological functions but also has the capability to promote angiogenesis. In comparison to growth factors, copper offers multiple advantages. It could be utilized in conjunction with other inorganic ions to create new intelligent biomaterials that simulate the bone microenvironment. The aim of this study is to evaluate the hemocompatibility of Copper Oxide with BSA for osteoblast application.
Materials and Methods: CuO nanoparticles were prepared by precipitation method using copper nitrate. 100mg of Bovine serum albumin (BSA) protein powder was added. SEM, FTIR and cell viability was assessed. The control taken was blood with a buffer. ‘S’ represents blood with the sample. Statistical analyses were performed to determine the significance of results.
Results:The scanning electron microscopy study indicates that the morphology was identified as rod-shaped and measured at 1μ in size. A viability of approximately 95.4% was observed in cells regarding cell attachment and toxicity. Improved cell attachment and proliferation were noted.
Conclusion: The study concludes that the prepared copper oxide nanoparticle with BSA was found to be highly compatible and can be taken up for bone regeneration applications
Effect of microwave and probe sonication processes on sol–gel‐derived bioactive glass and its structural and biocompatible investigations
No full textWound Healing Tissue Graft Using Prf Incorporated Pla Membrane
No full textPoly lactic acid (PLA) is a biocompatible and biodegradable polymer that has been extensively used in tissue engineering and medical devices. Platelet rich fibrin (PRF) is a fibrin matrix in which platelet cytokines, growth factors, and cells are trapped and may be released after a certain time and that can serve as a resorbable membrane. Platelet-rich fibrin (PRF) incorporated into a poly(lactic acid) (PLA) membrane is a potential approach for wound healing and tissue grafting.
Materials and methods: This procedure is done by mixing 6% alginate with 3% poly lactic acid and then dissolving it in 100ml of water. And then, freshly platelet rich fibrin is collected from whole blood. These both solutions are mixed together and blended for 3 hours to form a homogenous membrane.
Results and discussion: PLA based composites development is one of the
strategic methods for different problems related diseases. Blending of PLA membrane with PRF may provide balanced physical and biological properties. Platelets, which contain growth factors, play major roles in cell migration, proliferation, differentiation and angiogenesis and are associated with the tissue regeneration process. It can be used as a major method in fabrication of wound healing faster compared to other methods available.
Conclusion: This study concludes that the synthesized PLA with PRF membrane is effective in accelerating wound healing
On the investigation of structural and biological properties of 45S5 bioglass and β-tricalcium phosphate nanostructured materials
No full textEffect of Titania Concentration in Bioglass/TiO<sub>2</sub> Nanostructures and Its <i>In Vitro</i> Biological Property Assessment
No full textComparative analysis of candidal carriage rates in long-term and short-term COVID-19 patients: An RT-PCR study
No full textBackground: Most individuals who had COVID-19 infection recover completely. However, current research shows that 10–20 % of the population, especially immunocompromised and elderly people, endure numerous adverse effects and multiple complications associated with different organs and systems during its pathogenic cycle. Also, many fungal coinfections are seen as post-COVID dysbiosis, the most common fungal organism being Candida. This study aims to compare the candidal carriage rate of short-term and long-term affected SARS-COV-2 patients in saliva using PCR and compare it with healthy individuals. Results: The study included both male (15) and female (23) long-term and short-term post-COVID patients and healthy individuals [Male (5) and Female (15)]. The candidal carriage rate was increased in long-term COVID patients than in short-term COVID patients and healthy individuals (p < 0.001∗). Among long-term COVID patients, those who had oxygen assistance (p=0.04) and were admitted to the ICU (p=0.01) had relatively higher candidal carriage rates than those who didn't receive any intensive care procedures. Conclusion: COVID-19 significantly affects the oral microbiome, leading to dysbiosis and increased candidal carriage. The manifestation of fungal coinfections in post-COVID patients appears to be influenced by various factors, including oxygen support, catheterization, and immunosuppressive treatments. Thereby, early diagnosis and early intervention, along with health care providers acquainted with potential risks and the likelihood of secondary infections, are the only ways to reduce the consequences of this devastating disease
Exploration of thermal treatment dependent in-vitro mineralization on 45S5 bioactive nanostructured materials
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