973 research outputs found
Do neutral amino acids and their N-methylated analogues share the same Na-dependent carrier in brush-border membrane from the eel intestine?
No full text1. 1. Kinetics of Na-neutral amino acid cotransport by brush-border membrane vesicles from the eel (Anguilla anguilla) intestine was studied. 2. 2. The substrate-dependent Na-influx was estimated by monitoring the decay of a preset inside negative membrane potential with a voltage-sensitive fluorescent dye. 3. 3. N-Methylglycine, N-methylalanine and α-(methylamino)-isobutyric acid are transported by the Na-dependent carrier responsible for proline translocation. This carrier is distinct from the one responsible for glycine, alanine and α-aminoisobutyric acid translocation
sj-pdf-1-lup-10.1177_09612033211045056 – Supplemental Material for Premature rupture of membranes – A cause of foetal complications among lupus: A cohort study, systematic review and meta-analysis
No full textSupplemental Material, sj-pdf-1-lup-10.1177_09612033211045056 for Premature rupture of membranes – A cause of foetal complications among lupus: A cohort study, systematic review and meta-analysis by Flávia Cunha dos Santos, Marcela Lacerda Ignacchiti, Bruna Rodrigues, Luis Guillermo Velarde, Roger Abramino Levy, Guilherme Ramires de Jesús, Nilson Ramires de Jesús, Carlos Augusto Ferreira de Andrade and Evandro Mendes Klumb in Lupus</p
sj-pdf-3-lup-10.1177_09612033211045056 – Supplemental Material for Premature rupture of membranes – A cause of foetal complications among lupus: A cohort study, systematic review and meta-analysis
No full textSupplemental Material, sj-pdf-3-lup-10.1177_09612033211045056 for Premature rupture of membranes – A cause of foetal complications among lupus: A cohort study, systematic review and meta-analysis by Flávia Cunha dos Santos, Marcela Lacerda Ignacchiti, Bruna Rodrigues, Luis Guillermo Velarde, Roger Abramino Levy, Guilherme Ramires de Jesús, Nilson Ramires de Jesús, Carlos Augusto Ferreira de Andrade and Evandro Mendes Klumb in Lupus</p
sj-pdf-4-lup-10.1177_09612033211045056 – Supplemental Material for Premature rupture of membranes – A cause of foetal complications among lupus: A cohort study, systematic review and meta-analysis
No full textSupplemental Material, sj-pdf-4-lup-10.1177_09612033211045056 for Premature rupture of membranes – A cause of foetal complications among lupus: A cohort study, systematic review and meta-analysis by Flávia Cunha dos Santos, Marcela Lacerda Ignacchiti, Bruna Rodrigues, Luis Guillermo Velarde, Roger Abramino Levy, Guilherme Ramires de Jesús, Nilson Ramires de Jesús, Carlos Augusto Ferreira de Andrade and Evandro Mendes Klumb in Lupus</p
Plasma-treated PET surfaces improve the biocompatibility of human endothelial cells.
No full textFailures of small internal diameter vascular grafts have been caused by the lack of a stable endothelial lining to form on their artificial surfaces. Polymer surfaces can be optimized by means of proper treatment to allow a homogeneous and uniform coverage in artificial prosthesis applications. Several solutions were studied to improve cell attachment and growth on artificial materials. In the present study, polyethyleneterephthalate (PET) surfaces were treated by plasma processes with oxygen and ammonia and also in the presence of a gas mixture to verify the effect of functional groups grafting onto the endothelial cell growth. Related surface chemical modifications were investigated by X-ray photoelectron spectroscopy (XPS). Then using cytotoxicity and cytocompatibility tests, the biocompatibility of the modified PET surfaces was assessed by studying the behavior of human umbilical vein endothelial cells (HUVEC). The results showed that plasma- treated PET samples have no toxic effect on HUVEC. The cytocompatibility tests revealed an increase in cell growth with incubation time and the presence of well-spread and flattened cells (SEM analyses). Thus it is reported that plasma treatments can improve PET biocompatibility to HUVEC. (C) 2000 John Wiley and Sons, Inc.
Failures of small internal diameter vascular grafts have been caused by the lack of a stable endothelial lining to form on their artificial surfaces. Polymer surfaces can be optimized by means of proper treatment to allow a homogeneous and uniform coverage in artificial prosthesis applications. Several solutions were studied to improve cell attachment and growth on artificial materials. In the present study, polyethyleneterephthalate (PET) surfaces were treated by plasma processes with oxygen and ammonia and also in the presence of a gas mixture to verify the effect of functional groups grafting onto the endothelial cell growth. Related surface chemical modifications were investigated by X-ray photoelectron spectroscopy (XPS). Then using cytotoxicity and cytocompatibility tests, the biocompatibility of the modified PET surfaces was assessed by studying the behavior of human umbilical vein endothelial cells (HUVEC). The results showed that plasma-treated PET samples have no toxic effect on HUVEC. The cytocompatibility tests revealed an increase in cell growth with incubation time and the presence of well-spread and flattened cells (SEM analyses). Thus it is reported that plasma treatments can improve PET biocompatibility to HUVEC
sj-pdf-2-lup-10.1177_09612033211045056 – Supplemental Material for Premature rupture of membranes – A cause of foetal complications among lupus: A cohort study, systematic review and meta-analysis
No full textSupplemental Material, sj-pdf-2-lup-10.1177_09612033211045056 for Premature rupture of membranes – A cause of foetal complications among lupus: A cohort study, systematic review and meta-analysis by Flávia Cunha dos Santos, Marcela Lacerda Ignacchiti, Bruna Rodrigues, Luis Guillermo Velarde, Roger Abramino Levy, Guilherme Ramires de Jesús, Nilson Ramires de Jesús, Carlos Augusto Ferreira de Andrade and Evandro Mendes Klumb in Lupus</p
Study of the effects of pasteurization and selected microbial starters on functional traits of fermented table olives
No full textTable olives are one of the most known fruit consumed as fermented food, being a fundamental component of the Mediterranean diet. Their production and consumption continue to increase globally and represent an important economic source for the producing countries. One of the most stimulating challenges for the future is the modernization of olive fermentation process. Besides the demand for more reproducible and safer production methods that could be able to reduce product losses and potential risks, producers and consumers are increasingly attracted by the final product characteristics and properties on human health. In this study, the contribution of microbial starters to table olives was fully described in terms of specific enzymatic and microbiological profiles, nutrient components, fermentation -derived compounds, and content of bioactive compounds. The use of microbial starters from different sources was tested considering their technological features and potential ability to improve the functional traits of fermented black table olives. For each fermentation assay, the effects of controlled temperature (kept at 20 degrees C constantly) versus not controlled environmental conditions (oscillating between 7 and 17 degrees C), as well as the consequences of the pasteurization treatment were tested on the final products. Starter -driven fermentation strategies seemed to increase both total phenolic content and total antioxidant activity. Herein, among all the tested microbial starters, we provide data indicating that two bacterial strains (Leuconostoc mesenteroides KT 5-1 and Lactiplantibacillus plantarum BC T3-35), and two yeast strains (Saccharomyces cerevisiae 10A and Debaryomyces hansenii A15-44) were the better ones related to enzyme activities, total phenolic content and antioxidant activity. We also demonstrated that the fermentation of black table olives under not controlled environmental temperature conditions was more promising than the controlled level of 20 degrees C constantly in terms of technological and functional properties considered in this study. Moreover, we confirmed that the pasteurization process had a role in enhancing the levels of antioxidant compounds
The novel-essay in Eça de Queirós: critical study about A ilustre casa de Ramires and A cidade e as serras
No full textOs dois últimos romances escritos por Eça de Queirós, A ilustre casa de Ramires (1900) e A cidade e as serras (1901) publicados em livro apenas após a morte do autor continuam a desafiar a crítica queirosiana em razão do seu conteúdo e forma que os distanciam da produção anterior desse escritor português. A presente pesquisa analisou os dois romances a partir do marco metodológico da teoria crítica formulada pela Escola de Frankfurt, a fim de verificar se a forma adotada neles não estaria antecipando o romance-ensaio, gênero cujo amadurecimento se daria a partir das vanguardas europeias do século XX . Pôdese constatar pelos resultados da pesquisa que o uso da estrutura mise en abîme na primeira obra e de estratégias ensaísticas na segunda possibilitou ao autor um altíssimo rendimento literário e irônico, através do qual o leitor perspicaz tem acesso a uma representação crítica do empreendimento neocolonial e da ideologia burguesa que o justifica.The last two novels by Eça de Queirós, A ilustre casa de Ramires (1900), and A cidade e as serras (1901), both of them published in book form only after the author\'s death, continue to challenge the criticism, because of their content and form, which point to a dissociation between them and the previous work by this Portuguese writer. The present study has examined these two novels based on methodological point of view of critical theory, according to Frankfurt School. We searched to verify if the form adopted in these narratives would be an anticipation of the novel-essay, a genre whose maturity would occur in the European avantgarde in the twentieth century. The results of this research have indicated that the use of mise en abîme structure in the first novel, and of essayistic strategies in the second one confer the writer a very high literary and ironic result, by which the astute reader are able to explore a critical presentation of the neocolonial project, and bourgeois ideology that justifies it
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