1,721,567 research outputs found
Cleavable Linkers in Chemical Proteomics Applications
No full textThe discovery of the protein targets of small molecule probes is a crucial aspect of activity-based protein profiling and chemical biology. Mass spectrometry is the primary method for target identification, and in the last decade, cleavable linkers have become a popular strategy to facilitate protein enrichment and identification. In this chapter, we provide an overview of cleavable linkers used in chemical proteomics approaches, discuss their different chemistries, and describe how they aid in protein identification.status: Publishe
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Carbohydrates as chiral starting compounds in synthetic organic chemistry
No full textUBL - phd migration 201
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Targeting Platinum Compounds: synthesis and biological activity
No full textInspired by cisplatin, the inorganic drug discovered by Barnett Rosenberg in 1965, the research described in this thesis uses targeting ligands, or ligands varied in a combinatorial fashion, to find platinum complexes with more specific modes of action. These studies have lead to the development of novel (solid-phase) synthetic methods and to the discovery of several compounds with promising biological properties.UBL - phd migration 201
New cationic amphiphilic compounds as potential antibacterial agents
No full textHet onderwerp van het in dit proefschrift beschreven onderzoek is de ontwikkeling van nieuwe verbindingen met antibacteriële activiteit gericht tegen Gram-negatieve bacteri_n. Deze verbindingen zijn afgeleid van kationische antimicrobiële peptides (CAPs), een klasse van antibiotica die volgens andere mechanismen werkt dan de huidige antibiotica. Bepaalde eigenschappen van CAPs moeten geoptimaliseerd worden willen zij uiteindelijk tot klinisch toepasbaar antibioticum ontwikkeld worden. Zo wordt het niet-toxische CAP drosocine afgebroken in het lichaam voordat het zijn werk heeft kunnen doen. Het vervangen van een aantal aminozuren in drosocine leidde tot afgeleiden die tot 30x stabieler zijn. In een poging om de toxiciteit van het zeer effectieve CAP polymyxine B te verlagen met behoud van antibacteriële activiteit, werden een aantal afgeleiden gesynthetiseerd via een nieuwe syntheseroute. Net als polymyxine B bindt het polymyxine B nonapeptide fragment sterk aan LPS. Hierdoor wordt LPS geneutraliseerd en worden immunologische reacties tegengegaan. Het nonapeptide heeft de toxiciteit van polymyxine B verloren, maar helaas ook de antibacteriële activiteit. Om de LPS-bindende activiteit van het nonapeptide te combineren met antibiotische activiteit, werden conjugaten gemaakt waarin het nonapeptide en andere CAPs chemisch verbonden zijn. Deze conjugaten behielden hun LPS-bindende activiteit met behoud of verlaging van antibiotische activiteit. Quarternaire ammonium zouten, kationische stoffen die kleiner zijn dan CAPs, vertonen ook antibiotische activiteit. Twee nieuwe soorten zouten werden gesynthetiseerd. Het mengen van deze zouten met water en andere oplosmiddelen leidde tot gels die sterke antibiotische werking tegen Gram-negatieve bacteriën vertoonden.UBL - phd migration 201
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