484 research outputs found
Caracterizacion de las vías de tráfico intracelular dependientes de RabGTPasas involucradas en la replicación de VIH-1 en linfocitos T CD4+
No full textDurante las últimas etapas del ciclo de replicación del VIH-1, la poliproteína viral Pr55Gag es reclutada a la membrana plasmática, donde se une el fosfatidilinositol 4,5-bisfosfato [PI(4,5)P2] para iniciar el ensamblado de VIH-1. Las vías de tráfico intracelular requeridas para llevar adelante este proceso son poco conocidas, principalmente en Linfocitos T CD4+. En esta tesis doctoral nos propusimos estudiar el papel desempeñado en este proceso por las proteínas RabGTPasa, reguladoras centrales de las vías de tráfico intracelular. A través de un tamizaje con ARN de interferencias, demostramos que al menos 10 miembros de esta familia de proteínas son necesarios para la replicación viral. Mediante un minucioso estudio de biología celular, demostramos que la proteína Rab27a participa en la formación de un dominio de la membrana plasmática que funciona como plataforma para el ensamblado viral en linfocitos T CD4+. En efecto, Rab27a promueve la llegada de la enzima fosfatidilinositol 4-quinasa tipo 2 α (PI4KIIα) desde los endosomas tardíos hasta la membrana plasmática. En consecuencia, se produce un incremento localizado de los niveles de fosfatidilinositol 4-fosfato y subsiguientemente de PI(4,5)P2. Estos dominios ricos en PI(4,5)P2 son utilizados como sitio de reclutamiento de Pr55Gag, dando inicio de esta manera al ensamblado viral. A fin de adentrarnos aún más en el mecanismo responsable de la formación de la plataforma viral, investigamos e identificamos que tres efectores de Rab27a (Slac2-b, Slp3 y Slp2-a) son reclutados por esta GTPasa para llevar a cabo esta vía de tráfico.Fil: Pereyra Gerber, Federico Pehuén. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentin
Relatório final de estágio realizado na Escola Secundária Matias Aires
No full textEste relatório reflete o processo de formação e a experiência vivida ao longo do estágio pedagógico decorrido na Escola Secundária Matias Aires e na Escola Básica Dom Domingos Jardo, no ano letivo 2022/2023. Ficam aqui realçadas as tarefas desempenhadas e as atividades desenvolvidas ao longo deste processo. Procede-se também a uma análise dos métodos e estratégias a que se recorreu para a otimização quer do processo de ensino-aprendizagem dos alunos da turma 9.º 6 e quer da participação no desporto escolar.
O estágio constituiu uma componente curricular que deu azo a vários momentos de aprendizagem e a vários momentos de análise, que me permitiram atingir um aperfeiçoamento enquanto docente. Foram diversas experiências enriquecedoras, que fizeram com que este processo de aprendizagem fosse absolutamente decisivo para a minha adaptação ao ambiente escolar.
Todo este processo teve por base as competências expostas ou apresentadas no Guia de Estágio Pedagógico. Que estão estruturadas em 4 áreas de intervenção: (Área 1) Organização e gestão do ensino e da aprendizagem; (Área 2) Inovação e investigação pedagógica; (Área 3) Participação na escola; e (Área 4) Relação com a comunidade.This report reflects the whole learning journey and lives experiences during the author's teaching internship at Escola Secundária Matias Aires and Escola Básica Dom Domingos Jardo in the 2022/2023 school year. The tasks performed and the activities carried out throughout this process are highlighted here. An analysis is also made of the methods and strategies employed to optimize both the learning experience that students in a ninth-grade class go through and their involvement in school sports.
Internship is an integral part of the teaching curriculum, providing extensive opportunity for learning and reflection and allowing the author to develop and gain new skills. The lived experiences invaluably helped the author adapt to the school environment and play a role as an educator.
This project was developed in light of the guidelines published in Guia de Estágio Pedagógico. They are divided into four intervention areas: (area 1) Teaching and Learning Organization and Management; (area 2) Teaching Innovation and Research; (area 3) Involvement in School Activities; and (area 4) Relationship with the Community
Rab27a Supports Exosome-Dependent and -Independent Mechanisms That Modify the Tumor Microenvironment and Can Promote Tumor Progression
No full textDuring progression from single cancer cells to a tumor mass and metastases, tumor cells send signals that can subvert their tissue microenvironment. These signals involve soluble molecules and various extracellular vesicles, including a particular type termed exosomes. The specific roles of exosomes secreted in the tumor microenvironment, however, is unclear. The small GTPases RAB27A and RAB27B regulate exocytosis of multivesicular endosomes, which lead to exosome secretion, in human HeLa cells. Here, we used mouse models to show that Rab27a blockade in mammary carcinoma cells decreased secretion of exosomes characterized by endocytic markers, but also of matrix metalloproteinase 9, which is not associated with exosomes. Rab27a blockade resulted in decreased primary tumor growth and lung dissemination of a metastatic carcinoma (4T1), but not of a nonmetastatic carcinoma (TS/A). Local growth of 4T1 tumors required mobilization of a population of neutrophil immune cells induced by Rab27a-dependent secretion of exosomes together with a specific combination of cytokines and/or metalloproteinases. Our findings offer in vivo validation of the concept that exosome secretion can exert key pathophysiologic roles during tumor formation and progression, but they also highlight the idiosyncratic character of the tumor context.Fil: Bobrie, Angélique. Inserm; Francia. Universite Paris-Descartes; FranciaFil: Krumeich, Sophie. Inserm; FranciaFil: Reyal, Fabien. Centre National de la Recherche Scientifique; FranciaFil: Recchi, Chiara. Imperial College London; Reino UnidoFil: Moita, Luis F.. Universidade Nova de Lisboa; PortugalFil: Seabra, Miguel C.. Imperial College London; Reino Unido. Universidade Nova de Lisboa; PortugalFil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Théry, Clotilde. Inserm; Franci
Relatório final de estágio realizado na Escola Secundária Matias Aires
No full textEste relatório reflete o processo de formação e a experiência vivida ao longo do estágio pedagógico decorrido na Escola Secundária Matias Aires e na Escola Básica Dom Domingos Jardo, no ano letivo 2022/2023. Ficam aqui realçadas as tarefas desempenhadas e as atividades desenvolvidas ao longo deste processo. Procede-se também a uma análise dos métodos e estratégias a que se recorreu para a otimização quer do processo de ensino-aprendizagem dos alunos da turma 9.º 6 e quer da participação no desporto escolar.
O estágio constituiu uma componente curricular que deu azo a vários momentos de aprendizagem e a vários momentos de análise, que me permitiram atingir um aperfeiçoamento enquanto docente. Foram diversas experiências enriquecedoras, que fizeram com que este processo de aprendizagem fosse absolutamente decisivo para a minha adaptação ao ambiente escolar.
Todo este processo teve por base as competências expostas ou apresentadas no Guia de Estágio Pedagógico. Que estão estruturadas em 4 áreas de intervenção: (Área 1) Organização e gestão do ensino e da aprendizagem; (Área 2) Inovação e investigação pedagógica; (Área 3) Participação na escola; e (Área 4) Relação com a comunidade.This report reflects the whole learning journey and lives experiences during the author's teaching internship at Escola Secundária Matias Aires and Escola Básica Dom Domingos Jardo in the 2022/2023 school year. The tasks performed and the activities carried out throughout this process are highlighted here. An analysis is also made of the methods and strategies employed to optimize both the learning experience that students in a ninth-grade class go through and their involvement in school sports.
Internship is an integral part of the teaching curriculum, providing extensive opportunity for learning and reflection and allowing the author to develop and gain new skills. The lived experiences invaluably helped the author adapt to the school environment and play a role as an educator.
This project was developed in light of the guidelines published in Guia de Estágio Pedagógico. They are divided into four intervention areas: (area 1) Teaching and Learning Organization and Management; (area 2) Teaching Innovation and Research; (area 3) Involvement in School Activities; and (area 4) Relationship with the Community
B-Cell Epitopes in the Immunodominant p34 Antigen of Mycobacterium avium ssp. paratuberculosis Recognized by Antibodies from Infected Cattle
No full textMycobacterium avium ssp. paratuberculosis (M. paratuberculosis) causes Johne's disease, a chronic and fatal enteritis in ruminants. In the last stage of the disease, antibody titres rise and levels of interferon-γ decrease, suggesting that the host-immune response is switching from a T helper 1 (Th1) to a Th2 profile. In infected cattle, the membrane protein p34 elicits the predominant humoral response against M. paratuberculosis. To map the B-cell epitopes of this antigen, affinity-purified bovine antibodies against the carboxy-terminal region of p34 were used to screen a 12-mer phage display library. Several phage clones carrying peptides resembling fragments of p34 were affinity selected. Based on the predicted amino acid sequence, peptides were chemically synthesized, which demonstrated reactivity with serum from naturally infected and p34-vaccinated cattle. Immunization of mice with these peptides elicited an anti-p34 antibody response. Two B-cell epitopes were identified and characterized. Based on the reactivity and the type of immune response elicited, epitope A was determined to be conformational, whereas epitope B was demonstrated to be sequential. Both epitopes were shown to be present in p34 proteins from M. avium ssp. avium or M. paratuberculosis but absent from M. intracellulare, the other member of the M. avium complex. Furthermore, both epitopes were mapped to regions of p34 that display high variability when compared to homologous proteins from other mycobacterial species of public and animal health importance. We hypothesize that these variable regions of p34 may play a role in the immunobiology of M. paratuberculosis infections.Fil: Ostrowski, Matias. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Mundo, Silvia Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Harris, N. B.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Barletta, R. G.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Lopez, O. J.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentin
CVS-Vintage: A dataset of 14 CVS repositories of java software
No full text<p>CVS-Vintage is a dataset of 14 CVS repositories of Java applications. This dataset aims at supporting the replication of early papers in the field of software evolution and mining software repositories. By building this dataset, we saved some CVS repositories from a probable death by deletion.</p>
<pre>@techreport{monperrus:hal-00769121,
title = {CVS-Vintage: A Dataset of 14 CVS Repositories of Java Software},
author = {Monperrus, Martin and Martinez, Matias},
url = {https://hal.archives-ouvertes.fr/hal-00769121/file/paper.pdf},
year = {2012},
number = {hal-00769121},
institution = {HAL},
}</pre>
<p>A Git version is available at https://github.com/monperrus/cvs-vintage/</p>
The role of semen in sexual transmission of HIV: beyond a carrier for virus particles.
No full textUnprotected sexual intercourse between discordant couples is by far the most frequent mode of HIV-1 (human immunodeficiency virus type 1) transmission being semen the main vector for HIV-1 dissemination worldwide. Semen is usually considered merely as a vehicle for HIV-1 transmission. In this review we discuss recent observations suggesting that beyond being a carrier for virus particles semen markedly influences the early events involved in sexual transmission of HIV through the mucosal barriers.Fil: Sabatte, Juan Atilio. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia del Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Remes Lenicov, Federico. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia del Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Cabrini, Mercedes. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia del Sida; ArgentinaFil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia del Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ostrowski, Matias. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia del Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ceballos, Ana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia del Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Amigorena, Sebastián. Inserm; FranciaFil: Geffner, Jorge Raul. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia del Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentin
Land CSEM impulse responses in simple layered models
No full textWe argue that in a half space model with a single layer, the electric field impulse response at different offsets can be understood physically. Diffusive fields travel along paths like wavefields but are not localised in time. Each event that is measured comes from a path with the least attenuation, which corresponds to early arrival. The most prominent TE mode contribution to the detection of a subsurface layer comes from the coupling of the airwave with the subsurface. The most prominent contribution from the TM mode comes from the path through the resistive layer with almost vertical diffusion paths between the surface and the target. The arrival time of the peak of the event from a conductive layer can be understood from this reasoning. The arrival time of the peak of the event from a resistive layer can be understood from this reasoning when the offset is not too large compared with burial depth of the target layer. These predictions lead to estimates of depth to target layer with an error of less than 5% and the resistivity of the resistive layer of less than 50%. The information contained in the impulse responses suggests that inversion would yield good results.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Applied Geophysics and Petrophysic
The early protective thymus-independent antibody response to foot-and-mouth disease virus is mediated by splenic CD9+ B lymphocytes
No full textInfection of mice with cytopathic foot-and-mouth disease virus (FMDV) induces a rapid and specific thymus-independent (TI) neutralizing antibody response that promptly clears the virus. Herein, it is shown that FMDV-infected dendritic cells (DCs) directly stimulate splenic innate-like CD9+ B lymphocytes to rapidly (3 days) produce neutralizing anti-FMDV immunoglobulin M antibodies without T-lymphocyte collaboration. In contrast, neither follicular (CD9-) B lymphocytes from the spleen nor B lymphocytes from lymph nodes efficiently respond to stimulation with FMDV-infected DCs. The production of these protective neutralizing antibodies is dependent on DC-derived interleukin-6 (IL-6) and on CD9+ cell-derived IL-10 secretion. In comparison, DCs loaded with UV-inactivated FMDV are significantly less efficient in directly stimulating B lymphocytes to secrete TI antibodies. A critical role of the spleen in the early production of anti-FMDV antibodies in infected mice was also demonstrated in vivo. Indeed, either splenectomy or functional disruption of the marginal zone of the spleen delays and reduces the magnitude of the TI anti-FMDV antibody response in infected mice. Together, these results indicate that in addition to virus localization, the FMDV-mediated modulation of DC functionality is a key parameter that collaborates in the induction of a rapid and protective TI antibody response against this virus. Copyright © 2007, American Society for Microbiology. All Rights Reserved.Fil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Zabal, Osvaldo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Sadir, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Lopez, Osvaldo J.. Northern Michigan University; Estados Unido
Environmental application of basic oxygen furnace slag for the removal of heavy metals from leachates
No full textIndustrial waste is a major environmental concern nowadays, stimulating the thorough study of the minimization and recycling of solid wastes and of the containment and treatment of liquid contaminants. Basic oxygen furnace (BOF) slag, a solid waste from the steel industry, has been found to be effective in the removal of heavy metals. However, this has not been applied so far in low permeability barriers, such as those used as bottom liners in landfills. This work studies the performance of BOF slag in both containment and treatment technologies for toxic leachates. Flow models are developed to assess the transport of metal ions through a permeable reactive barrier and a composite clay barrier. Reactive transport through the slag barrier and adsorption in the clay barrier are coupled for different conditions to find the residence time, the barrier life span and the optimum operative conditions. The results show that the use of BOF slag increases the breakthrough time of the contaminants, enabling improve design of low and high conductivity reactive barriers, and expands the life cycle of the material.Fil: Francisca, Franco Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Estudios Avanzados en Ingeniería y Tecnología. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Estudios Avanzados en Ingeniería y Tecnología; ArgentinaFil: Glatstein, Daniel Alejandro. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
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