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Emerg Infect Dis
No full textAvian InfluenzaHans-Dieter Klenk, Mikhail N. Matrosovich, and Ju\u308rgen Stech, editorsMonographs in Virology, vol. 27, S. Karger AG, Basel, Switzerland, 2008ISBN-10: 3805585012 ISBN-13: 978-3-8055-8501-9 Pages: 292; Price: US $24
Detección rápida de ARN del SARS-CoV-2 mediante amplificación de la polimerasa recombinasa de transcripción inversa (RT-RPA) con flujo lateral para el gen de la proteína N y mutación de deleción-inserción específica de variante en el gen de la proteína S
Get PDFRapid molecular testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may contribute to the development of public health measures, particularly in resource-limited areas. Reverse transcription recombinase polymerase amplification using a lateral flow assay (RT-RPA-LF) allows rapid RNA detection without thermal cyclers. In this study, we developed two assays to detect the SARS-CoV-2 nucleocapsid (N) gene and Omicron BA.1 spike (S) gene-specific deletion–insertion mutations (del211/ins214). Both tests had a detection limit of 10 copies/μL in vitro and the detection time was approximately 35 min from incubation to detection. The sensitivities of SARS-CoV-2 (N) RT-RPA-LF by viral load categories were 100% for clinical samples with high (> 9015.7 copies/μL, cycle quantification (Cq): < 25) and moderate (385.5–9015.7 copies/μL, Cq: 25–29.9) viral load, 83.3% for low (16.5–385.5 copies/μL, Cq: 30–34.9), and 14.3% for very low (< 16.5 copies/μL, Cq: 35–40). The sensitivities of the Omicron BA.1 (S) RT-RPA-LF were 94.9%, 78%, 23.8%, and 0%, respectively, and the specificity against non-BA.1 SARS-CoV-2-positive samples was 96%. The assays seemed more sensitive than rapid antigen detection in moderate viral load samples. Although implementation in resource-limited settings requires additional improvements, deletion–insertion mutations were successfully detected by the RT-RPA-LF technique.Las pruebas moleculares rápidas para las variantes del SARS-CoV-2 pueden contribuir al desarrollo de medidas de salud pública, especialmente en áreas con recursos limitados. Las pruebas de amplificación isotérmica por polimerasas y recombinasas de transcripción inversa (RT-RPA-LF) permiten la detección rápida de ARN sin termocicladores. En este estudio, he desarrollado dos pruebas para detectar el gen de la nucleocápside (N) y las mutaciones de delecióninserción específicas del gen de la espiga (S) de la variante Omicron BA.1 (del211 /ins214). Ambas pruebas tienen un límite de detección de 10 copias/µL, un tiempo de detección de 35 minutos.
Las sensibilidades de SARS-CoV-2 (N) RT-RPA-LF según las categorías de carga viral fueron del 100% para muestras clínicas con carga viral alta (>9015.7 copias/µL, Cq: < 25) y moderada (385.5-9015.7 copias/µL, Cq: 25-29.9), 83.3% para carga viral baja (16.5-385.5 copias/µL, Cq: 30-34.9), y 14.3% para carga viral muy baja (< 16.5 copias/µL, Cq: 35-40). Las sensibilidades de Omicron BA. 1 (S) RT-RPA-LF fueron del 94.9%, 78%, 23.896, y 0%, respectivamente, y la especificidad contra muestras positivas para SARS-CoV-2 no-BA.1 fue del 96%. Ambas pruebas moleculares son más sensibles que las pruebas rápidas de antígenos en muestras con carga viral moderada.Japón. Ministerio de Educación, Cultura, Deportes, Ciencia y Tecnología (Monbukagakusho)Tesis doctora
The 81st Annual Meeting of the Society of Tokyo Women's Medical University Symposium "Globalization and Infectious Diseases in the 21st Century" (1) Globalization and Emerging Infectious Diseases
No full text第81回東京女子医科大学学会総会 平成27年10月10日(土) 東京女子医科大学弥生記念講
Emerg Infect Dis
No full textIt is 10 years since severe acute respiratory syndrome (SARS) emerged, and East and Southeast Asia retain a reputation as a hot spot of emerging infectious diseases. The region is certainly a hot spot of socioeconomic and environmental change, and although some changes (e.g., urbanization and agricultural intensification) may reduce the probability of emerging infectious diseases, the effect of any individual emergence event may be increased by the greater concentration and connectivity of livestock, persons, and products. The region is now better able to detect and respond to emerging infectious diseases than it was a decade ago, but the tools and methods to produce sufficiently refined assessments of the risks of disease emergence are still lacking. Given the continued scale and pace of change in East and Southeast Asia, it is vital that capabilities for predicting, identifying, and controlling biologic threats do not stagnate as the memory of SARS fades
Emerg Infect Dis
No full textBetter preparedness for an influenza pandemic mitigates its impact. Many countries have started developing and implementing national influenza pandemic preparedness plans. However, the level of preparedness varies among countries. Developing countries encounter unique and difficult issues and challenges in preparing for a pandemic. Deaths attributable to an influenza pandemic could be substantially higher in developing countries than in industrialized countries. Pharmaceutical interventions such as vaccines and antiviral agents are less likely to be available in developing countries. The public health and clinical infrastructure of developing countries are often inadequate to deal with a widespread health crisis such as an influenza pandemic. Such an event will inevitably have a global effect. Therefore, improving pandemic preparedness in every country, particularly developing ones, is urgently needed
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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