3,373 research outputs found
Immunity in vaccinated cattle exposed to experimental and natural infestations with Boophilus microplus
No full textWong J.Y.M. and Opdebeeck J.P. 1993. Immunity in vaccinated cattle exposed to experimental and natural infestations with Boophilus microplus. International Journal for Parasitology 23: 689-692. Gut membranes of the cattle tick Boophilus microplus were extracted using a low ionic strength buffer and the soluble antigens were used to vaccinate Hereford cattle. Following either experimental infestation with 40,000 larval ticks or exposure to field infestation, ticks from vaccinated cattle laid 54 and 75% fewer eggs, respectively, than ticks from control cattle. After cattle were given booster injections of vaccine, and either experimentally infested with 20,000 larval ticks or exposed to field infestation, the weight of eggs laid by ticks from vaccinated cattle was reduced by 80% and 52%, respectively. Acquired immunity to the ticks varied considerably in both vaccinated and non-vaccinated cattle
Immune responses of cattle to biochemically modified antigens from the midgut of the cattle tick, Boophilus microplus
No full textSummary Treatment of membrane antigens of the midgut (GM) of the cattle tick, Boophilus microplus with sodium metaperiodate (periodate). pronase and lipase significantly inhibited the reactivity of the GM with antibodies in the sera of 57 cattle vaccinated with GM. Treatment of GM with periodate only removed the correlation between antibody reactivity of sera and protection against infestation with licks. A monoclonal antibody (MoAb QU13). which recognises protective antigens solubilized from GM (Lee & Opdebeeck 1991), did not react with GM treated with penodate. Cattle vaccinated with GM extracts were significantly protected against infestation with cattle licks (P < 005), whereas cattle vaccinated with either GM extracts treated with periodate or with antigens precipitated from GM extracts with MoAb QU13 and also treated with periodate, were not protected against infestation. These studies provide preliminary evidence that protective antigens in the lick midgut membrane either are carbohydrate or arc dependent on carbohydrate for their specificity
Voice Compression and Communications: Principles and Applications for Fixes and Wireless Channels
No full textUp-to-date, expert coverage of topics in wireless voice communications Voice communication is the most important facet of mobile radio service. Even when the predicted surge of wireless data and Internet services becomes a reality, voice will remain the most natural means of human communication. Voice Compression and Communications details issues in wireless voice communications and treats compression, channel coding, and wireless transmission as a joint subject. Part I covers background material, whereas Part II provides detailed information on both proprietary and standardized analysis-by-synthesis codecs, including the speech codecs of virtually all existing wireline-based and wireless systems. Parts III and IV discuss mainly research-based wideband, audio, as well as very low-rate schemes likely to find their way into future standards. Voice Compression and Communications describes fundamental concepts in a non-mathematical way early in the book for those with only a background knowledge of signal processing and communications. More advanced readers will find detailed discussions of theoretical principles, future concepts, and solutions to various specific wireless voice communications problems
1973-10-25 Morehead State Concert and Lecture Series J.P. Donleavy
No full textRenowned author J.P. Donleavy speaks on the plight of an author and the methods to write, recorded on October 25, 1973
Entrainment and detrainment rates from the piv measurements at the top of laboratory analogs of stratocumulus and cumulus clouds
No full textWe analyze mixing at the top of laboratory analogs of convective clouds: stratocumulus and cumulus to investigate entrainment of environmental air into the cloud. We retrieve two components of air velocity using Particle Image Velocimetry technique. Suitable image processing allows to determine cloud–clear air interface. Using velocity differences between cloudy and clear sides of the interface we calculate entrainment / detrainment rates
Vaccines against blood-sucking arthropods
No full textThis paper provides selected personal insights on the development of vaccines against blood-sucking arthropods, with particular emphasis on vaccines against ticks. The emergence of novel or concealed antigens of haematophagous ectoparasites as candidate vaccine antigens is reviewed and the effect of feeding by the parasite on the expression of protective antigens is considered. The distribution of protective antigens through life cycle stages, the stage of the life cycle targeted by protective responses, and the nature of these responses, are commented on briefly. Concealed antigens of the gut, including the peritrophic membrane, and other internal organs, are evaluated for the role they play in induction of immunity artificially. Some of the work carried out to purify and characterise protective antigens of tick guts is described. A commentary is developed on vaccines that combine both "concealed" and "exposed" antigens. Some of the problems associated with the infestation and challenge of vaccinated hosts in the field are identified and the delivery of parasite antigens as vaccines that are both protective and "user-friendly" is emphasised as a major problem to be solved
Vortex Dynamics in The Transitional and Turbulent Wake of 6:1 Prolate Spheroid at 45-deg incidence angle
No full textThe incompressible flow past a 6:1 prolate spheroid with an inclination angle of 45o at Re = 3,000 has been studied by means of direct numerical simulations (DNS). The Reynolds number is based on the inflow velocity and minor-axis length. The preliminary results presented here are focused mainly on vortex dynamics and vortical structures in the wake. The wake behind this configuration starts almost symmetric but is soon strongly deflected and bent as it evolves to the intermediate wake. A pair of unequal-strength vortices dominates the intermediate wake, of which one exhibits the shape of a long vortex tube while the other rapidly breaks down into turbulent-like vortical structures
Experimental characterisation of large scale structures in a high Reynolds number turbulent boundary layer
No full textA very large field of view (4δ x 1δ) with a good spatial resolution owing to the use of four 2k x 2k pixel cameras was conducted in a flat plate boundary layer at two Reynolds numbers (Reθ ≈7,500 and 20,000). Comparing the flow statistics with previously obtained hot-wire data under similar flow conditions show good agreement. The goal of this experiment is to detect and characterise the large scale motions which develop in the log region of a high Reynolds number turbulent boundary layer
Letter from J.P. Bradley to Mr. [William] S. Martin The Dominguez Estate Company, June 28, 1940
No full textRegarding attached payment by Mr. K.L. Schaap settling his account
The effects of continuous and intermittent delivery of antigens of Boophilus microplus on the development of murine antibodies
No full textProtective antigens solubilised from the membranes of the midgut (LI-GM) of the adult cattle tick Boophilus microplus were delivered to mice either continuously for up to 4 weeks from osmotic pumps implanted subcutaneously or intermittently in a pulsatile fashion by injections or by a combination of both modes of delivery. The effects of delivery profile on antigen specific antibody levels and avidity were compared. LI-GM delivered either by three injections (weeks 0, 2 and 4), or continuously from osmotic pumps (over 4 weeks) induced similar levels of antibodies in mice. The mode of delivery of LI-GM when in the presence of the adjuvant Quil A did not generally affect either the level or the avidity of the antibody response; indeed a single injection of LI-GM in the presence of Quil A stimulated an immune response similar to that induced by several combinations of pulsatile and of continuous delivery where mice were exposed to antigen and adjuvant for up to 4 weeks. LI-GM incubated at 37°C in vitro and in vivo for periods from 4 h to 14 days was partly degraded into low molecular weight (less than 29 kDa) components. The immunogenicity of LI-GM incubated in vitro for 4 h was significantly decreased, although its antigenicity was not affected after incubation for up to 14 days. In conclusion, delivery of LI-GM continuously from osmotic pumps demonstrated that single-step immunisation of mice with tick antigens was feasible. However, it was also demonstrated that the continuous delivery of antigen was only advantageous (i.e potential for a decrease in the number of times an animal must be handled) compared with delivery by injections when no adjuvant was used. Further work is necessary to establish the effect antigen degradation has in limiting the immune response resulting from continuous delivery of antigen from osmotic pumps
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