171,271 research outputs found
"Un personaggio di fiaba generoso e gentile". O'Tama Kiyohara e l'amicizia con Mario ed Elisa Oliveri
No full textIl testo ricostruisce - anche attraverso alcuni documenti inediti - il quadro dei rapporti fra il poeta ed editore Mario Oliveri (1900-1963), lo scultore Vincenzo Ragusa e sua moglie, la pittrice O'Tama Kiyohara, dal 1925 sino al ritorno di O'Tama in Giappone, nel settembre 1932
Cinnamomum Oliveri F. M. Bailey Leaf Solvent Extractions Inhibit the Growth of a Panel of Pathogenic Bacteria
No full textIntroduction: Cinnamomum oliveri F. M. Bailey is a rain forest tree native to Australia. Decoctions, infusions and essential oils produced from the leaves were used traditionally to treat a variety of bacterial diseases. Despite this, C. oliveri leaf extractions have not been rigorously examined for antibacterial properties against many pathogens. Methods: The antimicrobial activity of C. oliveri leaf extractions was investigated by disc diffusion and growth time course assays against a panel of pathogenic bacteria. The growth inhibitory activity was quantified by MIC determination. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: C. oliveri leaf solvent extractions inhibited the growth of a wide range of bacterial species. Growth of both gram positive and gram negative bacteria was inhibited by the C. oliveri leaf extracts to approximately the same extent. The methanolic extracts were generally most potent growth inhibitors. The methanolic, aqueous and ethyl acetate C. oliveri leaf extracts were particularly potent inhibitors of P. mirabilis growth, with MIC values as low as 127 μg/mL (methanolic extract). A. coli, K. pneumoniae and B. cereus were also particularly susceptible to the methanolic, aqueous and ethyl acetate extracts, with MIC values generally substantially <1000 μg/ mL. The antibacterial activity of the methanolic C. oliveri leaf extract was further investigated by growth time course assays which showed significant growth inhibition in cultures of E. coli, K. pneumoniae and P. mirabilis within 1 h of exposure. All extracts were determined to be nontoxic in the Artemia franciscana nauplii bioassay, indicating their safety for internal use as well as for topical uses. Conclusions: The lack of toxicity of the C. oliveri leaf extracts and their growth inhibitory bioactivity against a panel of pathogenic bacteria partially validate the traditional usage of these species to treat bacterial diseases and indicate their potential in the development of antiseptic agents.Full Tex
DIENCEFALO: TALAMO-IPOTALAMO. TERMOREGOLAZIONE; REGOLAZIONE DI EQUILIBRIO IDRICO, APPORTO ALIMENTARE E CICLO MESTRUALE
No full textMolecular characterization of skeletal regeneration in the brittle star amphiura filiformis
No full textEchinoderms are well known for their extensive regenerative abilities, but have been neglected in the field due to the lack of available molecular tools and resources [1]. Recently, developmental [2] and adult transcriptomes [3, 4] of the brittle star Amphiura filiformis have been sequenced, which opened up this species for molecular investigations of its rapid arm regeneration process. We use this brittle star as a model to understand the cellular and molecular aspects of skeletogenesis during adult arm regeneration and the potential role of the FGF signalling pathway in this process. Ultimately, we compare the molecular network driving regeneration of the skeleton to that underlying embryonic skeleton development [5].
Following a characterization of the anatomy and development of the skeleton during arm regeneration in A. filiformis [6], we established methods for spatio-temporal expression analysis [7] and pharmacological treatments to characterise genes involved in adult arm regeneration. We found that 18 embryonic skeletogenic mesoderm genes (transcription factors, signaling receptors and downstream differentiation genes) are also expressed in the dermal layer of the adult regenerating arm, where skeletal spicules form. FGF signalling perturbation using the SU5402 inhibitor interferes with skeleton formation during both embryonic development and adult regeneration of this brittle star. A large-scale comparison of genes affected by SU5402 in adult arm regeneration and during embryonic development revealed a conservation of network components downstream of FGF signalling between those two developmental modes.
Acknowledgements: We thank the staff at the Sven Lovén Centre for Marine Sciences in Kristineberg, especially Olga Ortega-Martinez and Sam Dupont, for assistance during animal and sample collection.
References:
1. Dupont S, Thorndyke M (2007) Bridging the regeneration gap: insights from echinoderm models. Nat Rev Genet 8:8–10
2. Delroisse J, Ortega-Martinez O, Dupont S, Mallefet J, Flammang P (2015) De novo transcriptome of the European brittle star Amphiura filiformis pluteus larvae. Mar Genomics. doi: 10.1016/j.margen.2015.05.014
3. Purushothaman S, Saxena S, Meghah V, Swamy CVB, Ortega-Martinez O, Dupont S, Idris M (2014) Transcriptomic and proteomic analyses of Amphiura filiformis arm tissue-undergoing regeneration. J Proteomics 1–12
4. Delroisse J, Mallefet J, Flammang P (2016) De Novo Adult Transcriptomes of Two European Brittle Stars: Spotlight on Opsin-Based Photoreception. PLoS One 11:e0152988
5. Dylus DV, Czarkwiani A, Stångberg J, Ortega-Martinez O, Dupont S, Oliveri P (2016) Large-scale gene expression study in the ophiuroid Amphiura filiformis provides insights into evolution of gene regulatory networks. Evodevo 7:2
6. Czarkwiani A, Ferrario C, Dylus D V., Sugni M, Oliveri P (2016) Skeletal regeneration in the brittle star Amphiura filiformis. Front Zool 13:18
7. Czarkwiani A, Dylus D V., Oliveri P (2013) Expression of skeletogenic genes during arm regeneration in the brittle star Amphiura filiformis. Gene Expr Patterns 13:464–47
Taxonomic revision of the marbled skink (Cyclodina oliveri, Reptilia: skink (Cyclodina oliveri, Reptilia: description of a new species
No full textWe have completed a taxonomic revision of the New Zealand marbled skink (Cyclodina oliveri) species complex. Morphological analyses and mitochondrial sequence data (ND2, ND4, Cytochrome b; Total 1933 bp) are used to describe a new taxon (commonly known as the "Mokohinau" skink) and redefine C. oliveri. The morphological and molecular data indicate that C. oliveri is distributed on the Poor Knights Islands, Mercury Islands and Aldermen Islands. The new species is restricted to the Mokohinau Islands, Hen and Chickens group, Little Barrier Island and Great Barrier Island. Our data demonstrate that there is no support for the separation of the Poor Knights Islands population of C. oliveri from those on the Mercury Islands and Aldermen Islands. The genetic data indicate that C. whitakeri is part of the C. oliveri species group. Divergence time estimates indicate that the C. oliveri species complex diverged during the late-Miocene, with further divergences among island groups in C. oliveri including the origin of the new taxon during the late-Pliocene and mid-Pleistocene. We present a diagnostic key for Cyclodina
Informe Previo Grupo Minero de la Pampa de Olaen (Córdoba), Compañia Sominol
No full textFil: Oliveri, Jorge C. Ministerio de Industria. Dirección Nacional de Minería; Argentina.Carpeta 360, inéditos SEGEMA
Informe Previo del Grupo Minero San Virgilio (Wolfram), Departamento Calamuchita, Provincia de Córdoba
No full textCarpeta 444, inéditos SEGEMARFil: Oliveri, Jorge C. Ministerio de Industria. Dirección Nacional de Minería; Argentina
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