199,943 research outputs found

    African Olive (Olea europaea subsp. cuspidata) as an environmental weed in eastern Australia: a review

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    African Olive, Olea europaea subsp. cuspidata (Wall. ex G.Don) Cif. (family Oleaceae) is a dense-crowned tree introduced into Australia for horticulture in the mid 19th century. In recent decades, African Olive has become an aggressive woody weed, capable of forming a dense and permanent canopy in a wide range of vegetation types in south-west Sydney and beyond. Characteristics of African Olive invasion in south-west Sydney, and its seed dispersal by frugivorous birds are consistent with experience from Norfolk Island and Hawaii. We use records and aerial photographs from Mount Annan Botanic Garden and other bushland areas in south-west Sydney to describe the invasion stages and impacts of African Olive. The capacity for African Olive to establish in both temperate and subtropical zones, underlie the potential for spread well beyond current distribution in New South Wales. Research is now required to further develop control techniques and ecological restoration strategies for areas of heavy African Olive infestation. Mapping of current locations and a coordinated control strategy for African Olive is required to prevent future permanent loss of native plant diversity

    M. A. Olea, Introducciôn al derecho del trabajo

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    M. A. Olea, Introducciôn al derecho del trabajo. In: Revue internationale de droit comparé. Vol. 16 N°2, Avril-juin 1964. pp. 451-452

    M. A. Olea, Introducciôn al derecho del trabajo

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    M. A. Olea, Introducciôn al derecho del trabajo. In: Revue internationale de droit comparé. Vol. 16 N°2, Avril-juin 1964. pp. 451-452

    M. Alonso Olea De laservidumbreal contrato de trabajo

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    M. Alonso Olea De laservidumbreal contrato de trabajo. In: Revue internationale de droit comparé. Vol. 31 N°4, Octobre-décembre 1979. p. 889

    The Singular Evolution of Olea Genome Structure

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    The current view of plant genome evolution proposes that genome size has mainly been determined by polyploidisation and amplification/loss of transposons, with a minor role played by other repeated sequences, such as tandem repeats. In cultivated olive (Olea europaea subsp. europaea var. europaea), available data suggest a singular model of genome evolution, in which a massive expansion of tandem-repeated sequences accompanied changes in nuclear architecture. This peculiar scenario highlights the importance of focusing on Olea genus evolution, to shed light on mechanisms that led to its present genomic structure. Next-generation sequencing technologies, bioinformatics and in situ hybridisation were applied to study the genomic structure of five related Olea taxa, which originated at different times from their last common ancestor. On average, repetitive DNA in the Olea taxa ranged from ~59% to ~73% of the total genome, showing remarkable differences in terms of composition. Among repeats, we identified 11 major families of tandem repeats, with different abundances in the analysed taxa, five of which were novel discoveries. Interestingly, overall tandem repeat abundance was inversely correlated to that of retrotransposons. This trend might imply a competition in the proliferation of these repeat classes. Indeed, O. paniculata, the species closest to the Olea common ancestor, showed very few tandem-repeated sequences, while it was rich in long terminal repeat retrotransposons, suggesting that the amplification of tandem repeats occurred after its divergence from the Olea ancestor. Furthermore, some tandem repeats were physically localised in closely related O. europaea subspecies (i.e., cultivated olive and O. europaea subsp. cuspidata), which showed a significant difference in tandem repeats abundance. For 4 tandem repeats families, a similar number of hybridisation signals were observed in both subspecies, apparently indicating that, after their dissemination throughout the olive genome, these tandem repeats families differentially amplified maintaining the same positions in each genome. Overall, our research identified the temporal dynamics shaping genome structure during Olea speciation, which represented a singular model of genome evolution in higher plants

    Two new species of Chiropterotriton (Caudata: Plethodontidae) from northern Mexico

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    Rovito, Sean M., Parra-Olea, Gabriela (2015): Two new species of Chiropterotriton (Caudata: Plethodontidae) from northern Mexico. Zootaxa 4048 (1): 57-74, DOI: 10.11646/zootaxa.4048.1.

    Multiplicação in vitro de variedades de oliveira "Arbequina" e "Maria da Fé" em diferentes meios de culturas e fitorreguladores

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    Projeto acadêmico (graduação) - Universidade Federal de Santa Catarina. Campus Curitibanos. Ciências Rurais.A oliveira (Olea europaea L.) pertence à família Oleaceae, sendo cultivada pela sua produção de frutos para a extração do azeite de oliva e azeitonas de mesa, além da sua grande importância econômica, social e cultural. Tem ocorrência principalmente na bacia do mediterrâneo, sendo estendida para vários países do mundo. O Brasil é o segundo maior importador de azeite e o quarto maior importador de azeitonas de mesa. Dessa maneira, o desenvolvimento de pesquisas que promovam melhorias na produção e qualidade dos produtos pertinente, porém ainda são consideradas insuficientes. Como alternativa a essas limitações, o presente trabalho tem como objetivo estabelecer a micropropagação de variedades de oliveira adaptadas no Estado de Santa Catarina e Sul do Brasil. Para tanto, serão utilizadas técnicas que promovam a proliferação de brotações por organogênese direta, para a produção de mudas em grande quantidade. Para iniciação das culturas serão testados diferentes métodos de desinfestação, formulações salinas, diferentes níveis e tipos de fitorreguladores para promover maiores taxas de multiplicação. Antes da aclimatização e adaptação no ambiente serão avaliados diferentes métodos de indução do enraizamento. Esse projeto possibilitará a identificação de técnicas de cultivo para otimizar e reduzir custos, obtendo altas taxas de multiplicação, disponibilidade de mudas certificadas para estimular o cultivo no Brasil e com variedades de alta produtividade para extração do óleo e dos frutos com qualidade

    M. Alonso Garcia, Curso de derecho del trabajo, p. 400. M. Alonso Olea, Derecho del trabajo, p. 400. M. Ancel, Le divorce à l'étrange

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    M. Alonso Garcia, Curso de derecho del trabajo, p. 400. M. Alonso Olea, Derecho del trabajo, p. 400. M. Ancel, Le divorce à l'étrange. In: Revue internationale de droit comparé. Vol. 28 N°2, Avril-juin 1976. pp. 400-402

    Colletotrichum acutatum sensu lato : from diversity study to genome analysis

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    Colletotrichum acutatum sensu lato includes a number of important pathogens that cause economically significant losses of various crops. The C. acutatum species complex has a wide host range in both domesticated and wild plant species, and its capability to infect different types of hosts such as insects has also been described. Members belonging to this complex are able to develop three different types of interaction with plant hosts including biotrophic, necrotrophic and hemibiotrophic infections and are also capable of surviving on weeds and non-hosts without causing visible symptoms. They are mainly asexual, but some have a teleomorphic state called Glomerella and can be either homothallic or heterothallic. The sexual behaviour in Glomerella is more complicated than in most ascomycetes, and strains within the same species do not show a typical MAT1-1/2 system. The overall aim of this study was to gain an improved understanding of the relationships between the genetic diversity of global populations, host association patterns, geographic distribution and biological and pathological attributes. A database (CaITSdb) containing more than 800 rRNA sequences deposited in GenBank was created along with key biogeographic information, and the data have been analysed in order to investigate genetic diversity and distribution of sub-populations and their evolutionary relationships. The combined evidence was used to assemble a core collection of 120 isolates that are representative of the diversity in host preference, geographic origin, mating behaviour and molecular genetic variation. A multi-locus sequencing approach (based on four neutral loci) has been used to evaluate phylogenetic relationships amongst the isolates in the core collection. A strong relationship was observed between various genetic groups distinguished and their mating behaviour, geographic distribution and host association patterns. Oceania has been identified as a likely geographic origin of this pathogen, as the highest level of variability and groups related to a hypothetical ancestral population are mainly distributed in these countries. All homothallic isolates capable of producing perithecia belonged to the same genetic group A7; whereas all self-sterile heterothallic isolates were classified as either A3 or A5. Isolates derived from the same host tend to cluster together into genetic groups or sub-populations. This evidence is generally in agreement with recent published work on taxonomic re-assessment of Colletotrichum acutatum sensu lato, which indicates at least fifteen new species. This study has provided the evidence for the occurrence of three distinct genetic groups on strawberry in the UK corresponding to three species reported in the literature namely, C. nymphaeae, C. fioriniae and C. godetiae. Isolates belonging to the genetic groups that correspond to C. nymphaeae and C. fioriniae appeared to be the most aggressive on strawberry, followed by C. godetiae, and C. simmondsii (not found in the UK). Representative isolates of other species were less aggressive. The first whole genome sequence an isolate (A9 = C. simmondsii) from the C. acutatum sensu species complex was assembled and analysed using a range of bioinformatics algorithms. An isolate of C. simmondsii was chosen based on its wide host range including strawberry and the phylogeographic position. Genome analyses enabled prediction and annotation of the whole gene set at 13549 including 6 % unique to this species. The data also suggested an interesting expansion of several gene families, such as those encoding carbohydrate-active enzymes, secondary metabolites pathways and effectors which could be associated with the wide host range. The new knowledge and resources developed with the genome analyses along with the results of the population level diversity studies provide a platform for future comparative and functional genomics investigations to advance this research

    Molecular markers for cultivar characterisation in olea Olea europaea L.

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    Accurate identification of olive cultivars is an essential requirement for the oliveculture. Distribution of incorrectly labelled Olea europaea and the global spread of vegetative propagated cuttings over hundreds of years sometimes changed the names of the same cultivar growing in different regions. Development of cultivar specific markers is necessary for cultivars identification and protection and cultivar purity determination. Standard DNA marker types and universal database integrated with exiting agro-morphological identification could have a significant impact on the exact cultivar identification of olive plants. The putative synonymous Olea europaea cultivars Arancino, Carboncella, Corniolo, Moraiolo and Tondello have been characterised by different molecular methodologies. Cucca is another cultivar used for comparison. Analyses for olive cultivar identification were carried out through PCR amplification, restriction and cloning of spacers sequences of the nuclear ribosomal gene. The internal transcribed spacers region (ITS1, 5.8S and ITS2), was amplified by the polymerase chain reaction (PCR) by using two primers complementary to the 3’ region of 18S and 5’ region of 25S rDNA. The PCR product amplified from Moraiolo cultivar appears as a single band of approximately 700 bp in size: it was cloned using the pCR 2.1 vector. The ITS1 (about 250 bp), ITS2 (about 200 bp) and 5.8S rRNA gene were completely sequenced. No amplified length polymorphism was found among the analysed cultivars. The nuclear ribosomal intergenic spacer (IGS) and the flanking 5’ external transcribed spacer were amplified by using primers matching 3’ region of 25S and 5’ region of 18S. A partial sequence from Arancino cultivar was obtained. Amplicon restriction analyses were used to evaluate the genetic diversity among cultivars: no differential polymorphism was detected among them
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