819 research outputs found
白血病原因キナーゼであるFIP1L1-PDGFRAとSUMO化E3リガーゼであるPIAS1は、その酵素活性により正の相互作用を形成する
No full textCitation: Ibata Makoto, Iwasaki Junko, Fujioka Yoichiro, Nakagawa Koji, Darmanin Stephanie, Onozawa Masahiro, Hashimoto Daigo, Ohba Yusuke, Hatakeyama Shigetsugu, Teshima Takanori, Kondo Takeshi. (2016) Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Science 108 (2017) : 200-207. doi:10.1111/cas.13129配架番号：227
Budding of Ebola Virus Particles Requires the Rab11-Dependent Endocytic Recycling Pathway
No full textThe Ebola virus-encoded major matrix protein VP40 traffics to the plasma membrane, which leads to the formation of filamentous viral particles and subsequent viral egress. However, the cellular machineries underlying this process are not fully understood. In the present study, we have assessed the role of host endocytic recycling in Ebola virus particle formation. We found that a small GTPase Rab11, which regulates recycling of molecules among the trans-Golgi network, recycling endosomes, and the plasma membrane, was incorporated in Ebola virus-like particles. Although Rab11 predominantly localized in the perinuclear region, it distributed diffusely in the cytoplasm and partly localized in the periphery of the cells transiently expressing VP40. In contrast, Rab11 exhibited a perinuclear distribution when 2 VP40 derivatives that lack ability to traffic to the plasma membrane were expressed. Finally, expression of a dominant-negative form of Rab11 or knockdown of Rab11 inhibited both VP40-induced clusters at the plasma membrane and release of viral-like particles. Taken together, our findings demonstrate that Ebola virus exploits host endocytic recycling machinery to facilitate the trafficking of VP40 to the cell surface and the subsequent release of viral-like particles for its establishment of efficient viral egress
Women\u27s Cultural Education and Occupation in Serial Novels in the Early Showa Period : Focusing on Mother by Turumi Yusuke(1929)
No full textThis paper examines how the norm of good wife and wise mother was discussed through the usage of the word “educated” in Yusuke Tsurumi’s Mother, serialized in Fujin Club from May 1927 to June 1929. The Fujin Club, started in 1920 by Dai Nippon Yubenkai Kodansha, was a magazine read by many working women and girl students in their early teens to their early twenties. Through collaboration between Seiji Noma, president of Kodansha, who tried to present the mother as an ideal female figure, and author and politician Yusuke Tsurumi, the series Mother was started. It can be considered that Asako, the leading character of Mother, who overcomes various crises in life through ideal education obtained through reading and school, could garner the empathy of the women of those times who dreamt of being independent. However, since success as a working woman was for the progressive future of her son, Asako’s success ultimately gets attributed to the norm of good wife and wise mother. However, rather than seeing the series Mother of the Fujin Club as propaganda, the author would like to consider the significance of the series to lie in its potential as a text through which the Kodansha culture, considered a symbol of anti-cultural education strategically utilized the masses’desire for cultural education.査読研究論文Refereed Paper
Correction: Efficient oxygen evolution on mesoporous IrOx nanosheets (Catalysis Science and Technology (2019) DOI: 10.1039/c9cy00302a)
No full textThe spelling of the name of author Yusuke Yamauchi was incorrect in the original manuscript. The correct spelling is listed as shown above. The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers
Invasions of Small Numbers: How Many Virus Particles Does It Take to Infect Someone with the Flu?
No full textAbstract LB-323: Comparative miRNA microarray profiling indicates miR-363 promotes chemoresistance in ovarian cancer cells by targeting the Hippo member, LATS2
No full textAbstract
Ovarian cancer is the most aggressive female reproductive tract tumours. Taxane (TX) is widely used for ovarian cancer treatment. However, ovarian cancer often acquire chemoresistance. MicroRNAs (miR) have been reported to regulate many tumours’ chemoresistance. Here, we investigated the comparative microRNA expression profile between the ovarian cancer cells and their taxane-resistant counterpart, KF-TX to get novel markers for taxane resistance in ovarian cancer. The array data revealed that miR-486, miR143,miR145 and miR363 are the most upregulated miRNAs in association with TX resistance. However, miR-155, miR-100, miR-31 and miR-629 are the most significantly downregulated miRNAs after resistance development. We then focused on the role of miR-363 in chemoresistance of the ovarian cancer. qRT-PCR indicated that miR-363 was upregulated in KF-TX cells, confirming the array data, and introduction of miR-363 into sensitive ovarian cancer cells confers TX-resistance and significantly inhibited the expression of the Hippo member, LATS2, as indicated by viability, clonogenic assays and expression analysis. Furthermore, we validated the role of LATS2 in the TX-response by sh-based silencing, which also confers TX-resistance in the responsive ovarian cancer cells. On the other hand, specific inhibitor against miR-363 restored the response to TX. In addition, miR-363 was found to bind to the 3′-UTR of LATS2 mRNA, confirming that miR-363 directly targets LATS2 as indicated by dual luciferase assay. RT-PCR-based evaluation of miR-363 in a panel of human ovarian tumours revealed its upregulation in most of the tumour tissues identified as resistant while downregulation of the same gene in most of the tissues identified as sensitive ones. Moreover, higher levels of miR-363 in human ovarian cancer specimens were significantly correlated with TX chemoresistance and poor prognosis. Taken together, our study reveals the involvement of miR-363 in chemoresistance by targeting LATS2 in ovarian cancers, raising the possibility that combination therapy with a miR-363 inhibitor and TX may increase TX efficacy and reduce the chance of TX-resistance.
Note: This abstract was not presented at the meeting.
Citation Format: Noriaki Sakuragi, Mohamed Kamel Farah, Zeinab Mohamed, Safwat Okasha, Hidemichi Watari, Takashi Mitamura, sherif El Khamisy, Yusuke Ohba. Comparative miRNA microarray profiling indicates miR-363 promotes chemoresistance in ovarian cancer cells by targeting the Hippo member, LATS2 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr LB-323. doi:10.1158/1538-7445.AM2017-LB-323</jats:p
Deriving safety constraints for integration of unmanned aircraft systems into the national airspace by application of STECA
No full textThesis: S.M. in Technology and Policy, Massachusetts Institute of Technology, School of Engineering, Institute for Data, Systems, and Society, Technology and Policy Program, 2016.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Cataloged from student-submitted PDF version of thesis.Includes bibliographical references (pages 129-132).Unmanned aircraft systems (UAS) have been used for years especially in the military. However, the operation of UAS in civil aviation has been limited since there are a lot of uncertainties: a regulatory scheme needs to be established and associated technologies need to be developed. This thesis contributes to both technology development and establishing a regulatory scheme for UAS by generating safety constraints using the new methodology developed by Professor Leveson and Dr. Fleming. This methodology is called "'Systems-Theoretic Early Concept Analysis" (STECA) and is based on Systems-Theoretic Accident Model and Processes (STAMP) analysis, which is also developed by the professor. STECA has potential to generate more safety constraints that have not been considered otherwise in the early stage of development and this allows the producer to redesign the entire system with potentially less cost. This thesis illustrates why and how STECA can be powerful to support integration of UAS into NAS. In addition, this thesis actually demonstrates how STECA derives safety constraints as a case study and shows how the safety constraints should be integrated in the system development.by Yusuke Urano.S.M. in Technology and Polic
Visualization of Ras-PI3K interaction in the endosome using BiFC
No full textRecent studies indicate the importance of spatiotemporal regulation in the diversity and specificity of intracellular signaling. Here, we show that Ras-PI3K signaling plays an important role in the local regulation of phosphatidylinositol metabolism in the endosome through live-cell imaging by using a bimolecular fluorescence complementation technique, in which molecular interaction is indicated by fluorescence emission. Using several possible combinations of Ras and the Ras-binding domain, we identified an optimal set of probe molecules that yielded the most significant increase in fluorescence intensity between the active and inactive forms of Ras. This combination revealed that, among the Ras effectors tested, phosphatidylinositol 3-kinase (PI3K) was specifically implicated in signaling in the endosome. We also found that full length PI3K was recruited to the endosome in EGF- and Ras-dependent manners, which appears to be essential for the activation of PI3K in this compartment. Taken together, these findings demonstrate that the spatiotemporal regulation of Ras-PI3K signaling may dictate the activation of PI3K and subsequent downstream signaling in the endosome
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