1,720,974 research outputs found
I geni alla base della colorazione del mantello nel cane e prospettive di studio per la razza Bracco Italiano
No full textRassegna sullo studio dei geni di colorazione del mantello del cane e i relativi risultati ottenuti mediante la genetica molecolare
Mc1r, Agouti signaling protein (ASIP) and CBD103 are involved in brindle coat colour of Boxer and Great Dane dogs
No full textIn most mammals, variations in skin and hair colour (brown/black eumelanin and the red/yellow phaeomelanin) are mainly achieved by the interaction between two genes: Agouti signaling protein (ASIP) and melanocortin 1 receptor (Mc1r) encoding a ligand-receptor system that controls the eumelanin/pheomelanin switching. In domestic dogs, pigment type-switching is also controlled by an unexpected allele (CBD103) of K locus encoding a secreted protein (β-defensin) previously studied for its role in immunity. Interaction studies reveal that Mc1r is epistatic to variation at
Agouti and K loci. In this work, we examined Mc1r, ASIP and CBD103 as candidate genes for brindle or fawn Boxer and Great Dane animals. The brindle phenotype in domestic dogs consists of an irregular pattern of red-yellow stripes alternating with black-brown. We found that all brindle dogs are heterozygous for ΔG23 mutation
previously reported by Barsh’s team. In addition, we report for the fi rst time two interesting insertions in Agouti promoter region. The innovation of this work is the discovery of new alternative transcripts of Agouti that could be involved in brindle coat colour pattern in dogs. The interaction between CBD103 mutation and Agouti insertions promoting the different transcripts is discussed. This can be a starting point in better understanding their involvement in pigment switching leading to the brindle pattern formation
Characterization of different 5’-untraslated exons of ASIP gene in black-and tan Doberman Pinsher and brindle Boxer dogs
No full textDifferential expression of the ASIP gene and its interaction with MC1R have provided basic insight into pigment-type switching in mammals. Here, we report the characterization of a specific red-haired skin transcript and a specific black-haired skin transcript in the ASIP gene in the black-and-tan Doberman Pinscher. It is also shown that the brindle-haired skin of the Boxer exhibits a deregulated expression resulting in various 5′-untranslated exons. Comparative sequence analysis revealed a short interspersed element and a poly(A) stretch inserted within the promoter region of the ASIP in the Boxer. Genotyping studies have shown that both insertions are also present in brindle and fawn animals of the Boxer and Great Dane breeds. Furthermore, we genotyped MC1R and K loci for their known variants that affect coat color in dogs. As expected, all animals were homozygotes (EM/EM) for the mask mutation, and fawn animals were ky/ky. Unexpectedly, we found that all brindle animals were heterozygotes kB/ky. Our results suggest that differential expression of ASIP determine pigment-type switching in a MC1R and K allele-dependent manner in dogs
Caracterization of different 5’ ultranslated exons of the ASIP gene in blak-and-tan Doberman Pinscher and brindle Boxer dogs
No full textDifferential expression of the ASIP gene and its interaction with MC1R have provided basic insight into pigment-type switching in mammals. Here, we report the characterization of a specific red-haired skin transcript and a specific black-haired skin transcript in the ASIP gene in the black-and-tan Doberman Pinscher. It is also shown that the brindle-haired skin of the Boxer exhibits a deregulated expression resulting in various 5'-untranslated exons. Comparative sequence analysis revealed a short interspersed element and a poly(A) stretch inserted within the promoter region of the ASIP in the Boxer. Genotyping studies have shown that both insertions are also present in brindle and fawn animals of the Boxer and Great Dane breeds. Furthermore, we genotyped MC1R and K loci for their known variants that affect coat color in dogs. As expected, all animals were homozygotes (E(M) /E(M) ) for the mask mutation, and fawn animals were k(y) /k(y) . Unexpectedly, we found that all brindle animals were heterozygotes k(B) /k(y) . Our results suggest that differential expression of ASIP determine pigment-type switching in a MC1R and K allele-dependent manner in dogs
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Investigation of the Agouti gene for the identification of useful markers for coat colour association studies in domestic rabbits
No full textNew insights on coat colour genetics in rabbit coming from candidate gene analysis - Nuove acquisizioni della genetica molecolare sul determinismo del colore del mantello nel coniglio
No full textWe recently showed that mutations in the rabbit MC1R gene are associated with coat colours in different breeds. Here, we completed the characterization of the MC1R gene and further supported the association of the 30 bp deletion in this gene with the production of pheomelanic coat colours. Moreover, we identified 4 single nucleotide polymorphisms in the agouti (agouti signaling protein, ASIP) gene. These markers will be useful in association studies with coat colour in different rabbit breed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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