1,720,989 research outputs found
Chromosome rearrangements associated with CAD gene amplification. Experiments with cell hybrids.
No full textThe presence of high-risk chromosome aberrations in chronic lymphocytic leukaemia does not correlate with centrosome aberrations.
No full textChromosome aberrations are frequently found in B-cell chronic lymphocytic leukaemia (B-CLL), and specific chromosome aberrations identify poor prognostic subgroups. Almost all the aberrations identified in B-CLL involve loci where genes with a role in the regulation of centrosome duplication have been mapped. Centrosome aberrations have been described as a possible cause of numerical chromosome abnormalities in both solid and haematological tumours. However, little is known about the possible role of centrosome aberrations in B-CLL. To investigate whether centrosome aberrations do occur in B-CLL and correlate with cytogenetically defined prognostic subgroups, we examined a set of 64 B-CLL samples by immunofluorescent staining. B-CLL cases differed significantly from controls in the mean frequency of cells with centrosome aberrations, while no difference was found between subgroups with or without specific chromosome aberrations. Our results indicated that although centrosome aberrations were a common feature in B-CLL, they did not represent a reliable prognostic marker
PRIMA-1 induces autophagy in cancer cells carrying mutant or wild type p53.
Full text linkPRIMA-1 is a chemical compound identified as a growth suppressor of tumor cells expressing mutant p53. We previously found that in the MDA-MB-231 cell line expressing high level of the mutant p53-R280K protein, PRIMA-1 induced p53 ubiquitination and degradation associated to cell death. In this study, we investigated the ability of PRIMA-1 to induce autophagy in cancer cells. In MDA-MB-231 and HCT116 cells, expressing mutant or wild type p53, respectively, autophagy occurred following exposure to PRIMA-1, as shown by acridine orange staining, anti-LC3 immunofluorescence and immunoblots, as well as by electron microscopy. Autophagy was triggered also in the derivative cell lines knocked-down for p53, although to a different extent than in the parental cells expressing mutant or wild type p53. In particular, while wild type p53 limited PRIMA-1 induced autophagy, mutant p53 conversely promoted autophagy, thus sustaining cell viability following PRIMA-1 treatment. Therefore, the autophagic potential of PRIMA-1, besides being cell context dependent, could be modulated in a different way by the presence of wild type or mutant p53. Furthermore, since both cell lines lacking p53 were more sensitive to the cytotoxic effect of PRIMA-1 than the parental ones, our findings suggest that a deregulated autophagy may favor cell death induced by this drug
Chromosomal aberrations evaluated by CGH, FISH and GTG-banding in case of AIDS related Burkitt ‘s lymphoma.
No full textChromosome aberrations evaluated by comparative genomic hybridization in B cell chronic lymphocytic leukemia: correlation with CD38 expression
No full textChromosome aberrations evaluated by CGH in B-cell chronic lymphocytic leukemia: correlation with CD38 expression.
No full textTaxanes from shells and leaves of Corylus avellana
No full textPaclitaxel is an effective antineoplastic agent originally extracted in low yield from the bark of Taxus breVifolia. Although it was generally considered a particular metabolite of Taxus sp., paclitaxel was recently found in hazel cell cultures.
The aim of the present work was to verify whether hazel differentiated tissues could be used as a commercial source
of paclitaxel and other taxanes. Thus, shells and leaves of hazel plants were analyzed by ELISA and HPLC-MS. Both
shell and leaf extracts contained taxanes. Among these, paclitaxel, 10-deacetylbaccatin III, baccatin III, paclitaxel C,
and 7-epipaclitaxel were identified and quantified. Hazel extracts also showed biological activity, inhibiting metaphase
to anaphase transition in a human tumor cell line. The level of total taxanes in leaves was higher than in shells collected
in the same period from the same plants. However, the finding of these compounds in shells, which are considered
discarded material and are mass produced by many food industries, is of interest for the future availability of paclitaxel and other antineoplastic compounds
Uncommon cytogenetic findings in a case of splenic marginal zone lymphoma with aggressive clinical course
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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