1,721,087 research outputs found
Bacillus cereus nella filiera latte. Atti Corso Annuale di Perfezionamento “La nuova cultura delle produzioni animali nel contesto dell’unione europea”
No full textCaratteri di antibiotico-resistenza in ceppi di Salmonella enterica e Yersinia enterocolitica isolati da carne macinata destinata all'alimentazione del cane
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Attualità sulle infezioni da Aeromonas spp. Atti Corso Annuale di Perfezionamento “La nuova cultura delle produzioni animali nel contesto dell’unione europea”
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Flora batterica autoctona nei prodotti della pesca: un problema igienico sanitario emergente.
No full textMOLECULAR EVALUATION OF THE ENTEROTOXIGENICITY OF CLOSTRIDIUM DIFFICILE AND CLOSTRIDIUM PERFRINGENS SWINE ISOLATES BY PCR ASSAYS
No full textClostridium difficile and C. perfringens are enteric pathogens affecting a variety of mammals. This study evaluated the molecular enterotoxigenicity of Clostridium swine isolates by PCRs.
One hundred and ten swine faeces were analyzed by culture assay. The faecal samples were from sixty-seven healthy animals and 43 with gastrointestinal tract disease. C. difficile strains were PCR-screened for the presence of tcdA/tcdB and cdtA/cdtB genes. All C. perfringens isolates were tested for the characterization of the toxinotype.
Overall, sixty-five swine resulted positive: 38 for C. difficile and 17 for C. perfringens. One sample tested C. perfringens and C. difficile-positive, at the same time: on the whole, 39 C. difficile strains were isolated. Thirty-eight C. difficile isolates (all from healthy animals) resulted tcdA/tcdB and cdtA/cdtB-negative by PCRs and toxins A/B-negative by immunological tests. All C. perfringens strains were type A; eight were also cpb2-positive. In the sample (diarrhoeic), with double infection, C. difficile tested tcdA/tcdB and cdtA/cdtB-positive by PCRs and toxins A/B-positive by immunoassays; C. perfringens resulted cpb2-positive.
The molecular genotyping/toxinotyping should be applied to establish a final diagnosis and to assess properly the full implications and the epidemiological impact of these findings in particular in samples of healthy animals and aid in the development of effective intervention methods for controlling clostridial disease outbreaks
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