1,721,568 research outputs found
Combining chicken retina RNA-seq data across studies to strengthen biomarker detection
Get PDFVarious studies have identified the chicken embryo (Gallus gallus) as a useful model to study the retinogenesis process in humans. This project uses data from two specific RNA sequencing (RNA-seq) studies to investigate retina developmental biology. These studies are done in two different labs using different protocols, as such they cannot be compared directly. Study 1 contains chicken retina samples from embryonic day 3, 5 and 8; while study 2 has retina samples from embryonic day 8, 16, and 18 of developmental age. We apply a normalization method on both studies to account for differences in the two studies. In this work, we perform gene expression analysis on the transformed data to identify genes that could affect the retina development process
Effects of ferrous toxicity on seedling traits and ion distribution pattern in upland and low land rice under hydroponic conditions
No full textEnvironmentally, nutritional disorders are the major constraints due to the change in soil pH for
rice crop cultivated under different irrigation system. Iron toxicity is one of the famous nutritional
disorders caused by the excessive uptake of ferrous ion in low pH under aerobic conditions. This
research study was focused to measure the effect of different levels of ferrous stress on seedling
traits, distribution of ferrous, potassium ions in two upland rice and three lowland rice varieties.
The mean comparison of growth parameters were formulated using least significant differences,
as well as the experimental treatments were compared by analysis of variance. Varieties showed
significant differences for growth parametershowever, lowland rice varieties like Pokkali and
Firat exhibited the minimum deterioration in growth parameters. The root length was significantly
reduced under ferrous stress in all varieties. Ferrous accumulation was high in roots compared to
aerial plant parts and flag leaves exhibited the minimum accumulations. Reduction of potassium
reflected in all plant parts when an increased in stress was imposed. Upland rice varieties were
found sensitive compared to lowland rice varieties. It is thereforeconcluded that the growth
parameters would be a reflecting index for ferrous toxicity in rice. Furthermore, the availability
and uptake of potassium is improved, it may minimize the effects of ferrous toxicity
Abstract 3771: Concurrent GVAX and anti-OX40 therapy in murine intracranial glioma model
No full textAbstract
Introduction: Malignant glioma is the most aggressive brain tumor with a median survival time of less than 1.5 years post diagnosis despite standard treatments. However, recent advancements in immunotherapy hold significant promise. Irradiated whole tumor cell vaccination stimulates antitumor immunity and prolongs survival in mice with intracranial glioma tumors. We have shown that vaccination and immune checkpoint blockade is synergistic and these efforts are underway in the clinic. In contrast to immune checkpoint blockade, agonist stimulation of OX40, a member of the TNFR/TNF superfamily that is expressed on activated CD4 and CD8 T lymphocytes increases T cell proliferation, clonal memory differentiation, and cytokine release. We hypothesized that subcutaneous vaccination with irradiated GM-CSF expressing glioma cells (GVAX) would, therefore, synergize with systemic delivery of stimulating anti-OX40 antibody and provide effective immunity against intracranial gliomas.
Methods: Gl261 cells were implanted in the right frontal lobes of syngeneic c57/bl6 mice using a stereotactic implantation technique. Mice received concurrent subcutaneous injection of irradiated GM-CSF expressing glioma cells and intraperitoneal injection of OX40 antibody, either monotherapy, or control treatment on days 3, 6 and 9 following tumor implantation.
Results: Combination immunotherapy with irradiated GM-CSF expressing glioma cells and intraperitoneal delivery of agonist monoclonal antibody against OX40 was more effective than either therapy alone and prolonged survival in mice with established intracranial GL261 tumors. Elispot analyses of peripheral lymphocytes revealed that combination immunotherapy reverses the Th2 cytokine skew that is induced by the presence of intracranial glioma. OX40 signaling alone and in combination with vaccination drove high levels of interferon gamma expression by splenocytes harvested from glioma-bearing animals, while IL-10 expression was little changed. In the tumor microenvironment, vaccination improves the CD8+ /FoxP3+ T lymphocyte ratio, while OX40 ligation overcomes tumor-driven lymphocyte exhaustion, demonstrated by reduced expression of PD1 and LAG3, measured by flow cytometery 2 weeks after initiation of treatment.
Conclusions: Combination treatment with GVAX and OX-40 agonist antibody increased survival in mice bearing intracranial gliomas and are mechanistically complementary.
Citation Format: Nusrat Jahan, Hammad Talat, William T. Curry. Concurrent GVAX and anti-OX40 therapy in murine intracranial glioma model [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3771. doi:10.1158/1538-7445.AM2017-3771</jats:p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
i ECONOMIC IMPACT ANALYSIS OF HIGHWAY INVESTMENT PROJECTS By
No full textwritten under the direction o
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
글로벌 가치사슬이 산업 개발에 미치는 영향
No full text학위논문 (석사)-- 서울대학교 대학원 : 국제대학원 국제학과(국제통상전공), 2019. 2. Kim, Chong-Sup.생산과 최종 소비가 동시에 발생하지 않는 세계화 시대에는 발생하는 가치가 실제로 누구의 몫인지에 대한 우려가 커지고 있다. 글로벌 가치 사슬과 복식부기의 부상은 무역, 정책, 그리고 투자에 있어 중요한 주제로 떠오르고 있다. 정보를 확인하기 위한 방법은 한정적이며 개발 및 소득 수준을 고려하며 글로벌 가치사슬이 실제로 경제 개발에 미치는 영향을 파악하는 철저한 작업은 아직 이루어지지 않았습니다. OECD와 WTO의 도움으로 우리는 이제 복잡한 데이터 세트에 노출되었습니다. 이 연구는 대상 국가들의 글로벌 가치사슬의 참여도와 산업 발달의 인과 관계에 대하여 확인하였습니다. 기존의 저자들은 주로 GVC 지표를 개발하는 데에 중점을 두었습니다. 이 실증 연구는 2 차 자료의 데이터 세트를 사용하여 개발 및 소득 수준과 무관하게 세계 가치 사슬의 참여와 국가 차원의 산업 발전 사이에는 매우 강한 양의 상관관계가 있음을 발견하였습니다. 더 나아가 개별 산업들의 성과/영향들이 그 산업의 개발에 미치는 영항을 측정하여 대체적인 인과관계와 영향을 확인하였습니다.
실마리어: 글로벌 가치 사슬, 산업 부가가치, 경제 개발
학생증: 2016-26738In this era of the globalisation, where production and final consumption doesnt take place simultaneously increasing concerns are there regarding who actually captures value. The rise of Global Value Chain with double accounting has become one of the prominent issues in trade, policy and investment. The alley to know the facts are really narrow and also their actual impact on the economic development have not been ascertained rigorously yet, considering the level of development and income. Thereby with the help of OECD and WTO, we are now exposed to the complex data set. In my research I have studied the causal relationship of global value chain participation and its effect on the industrial development of concerned countries. Most of the authors till now have mainly focused on developing GVC indicators. Using data sets from secondary sources, in my empirical research I have found out that there is a very significant strong positive relationship between the participation of global value chain on country level industrial development irrespective to the level of income and development. In an extension in my research I have also measured the individual industries performances/impacts to that of their industrial development which also gives us the idea about the causal relationship and the impact of individual industrial sectors to their industrial development and growth at a large.
Keywords: Global Value Chain, Industry value Added, Economic Development
Student ID: 2016-26738CHAPTER 1: INTRODUCTION 1
CHAPTER 2: LITERATURE REVIEW. 6
CHAPTER 3: EMPIRICAL STUDY. 33
CHAPTER 4: LIMITATION 61
CHAPTER 5: CONCLUSION AND POLICY IMPLICATION 62
REFERENCES. 65
APPENDIX. 69
ABSTRACT (KOREAN) 71Maste
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