8,734 research outputs found
Differentiation of mouse embryonic stem cells along a hepatocyte lineage
No full textThe development of implantable engineered liver tissue constructs, ex vivo hepatocyte based therapeutic devices and drug discovery studies are limited by an inadequate hepatocyte cell source. Embryonic stem (ES) cells, characterized by their self-renewing and multi-lineage differentiating capabilities, represent a promising mature cell source required for these applications. Previous research has utilized embryoid body (EB) formation in both guided, through extracellular matrix and growth factor supplementation, and unguided, or spontaneous, differentiation to generate hepatocyte like cells. However, these characterizations have been limited to only one or several lineage specific protein or gene expression patterns. In addition, there have been few reports of long term propagation or characterization of long term function for ES cell derived hepatocyte precursors. In this thesis, we have implemented a platform for the long term propagation and augmentation of functional hepatocytes generated from murine ES cell sources. We first utilize a controlled, reproducible, EB mediated differentiation system to characterize efficiency of hepatocyte lineage commitment in four parallel culture configurations. These studies have shown that, EB mediated stem cell differentiation spontaneously yield populations of hepatocyte lineage cells expressing mature hepatocyte markers such as albumin (ALB) and Cytokeratin 18 (CK-18). We then used secondary culture configurations to study the effects of collagen sandwich culture and Oncostatin-M (OSM) or S-nitroso-N-acetylpenicillamine (SNAP) supplementation of EB derived hepatocyte-lineage cell function. The results of these studies suggest that SNAP, independent of the collagen supplementation, maintains the highest levels of ALB expression, however mature liver specific CK-18 is only expressed in the presence of both gel sandwich culture supplemented with SNAP. In addition, albumin secretion and Cytochrome P450 detoxification studies indicated that this condition was the best for the augmentation of hepatocyte-like function. Maintenance and augmentation of hepatocyte-like cells isolated from heterogeneous EB cell populations will be a critical step in generating large numbers of functional differentiated cells for therapeutic use.Ph.D.Includes bibliographical references (p. 45-48)
ROSENTHAL, Eric Inventory of documents
No full textCOVERAGE 1904; 1 File; 011 metre.Private papers of Eric Rosenthal, author, journalist and broadcaster
tritrophic-dispersal-model: Code used for creating figures for "Non-hierarchical dispersal promotes stability and resilience in a tri-trophic metacommunity"
No full text<p>This is the commented code used for creating figures for the paper. Any questions regarding the code should be directed to the corresponding author and repository owner (Eric Pedersen). </p>
Eric Velazquez Spanish Language Picture Book Award 2022 Acceptance Speech
No full textAuthor Eric Velazquez gives his Silver Medal acceptance speech for Pulpo Guisado (Holiday House)https://educate.bankstreet.edu/spanishlanguageaward/1001/thumbnail.jp
Eric C. Lincoln, Professor of Sociology and Religion, 1971
No full textThis is an interview with Eric C. Lincoln. Eric was a Professor of Sociology and religion, Union Theological Seminary and author of many books and articles on Negro history. In this recording the contributors discuss local memphis politics, sociology, and race relations compared to that of other cities in the South and the rest of the country
Interview with Eric Bentley, author, drama critic, and playwright
No full textDistinguished drama critic and Bertolt Brecht scholar, Eric Bentley is interviewed by WTMJ-TV host Jim Peck and John B. Fuegi, associate professor of Comparative Literature. Bentley recalls his association with Brecht, the critical and creative aspects of literature, and his interest in writing plays for the theater.GrayscaleSoun
Dr. Eric Yellin – Faculty Author Interview
No full textDr. Eric Yellin, Associate Professor of History and American Studies discusses his new book, Racism in the Nation’s Service: Government Workers and the Color Line in Woodrow Wilson’s America, published recently by the University of North Carolina Press. In this book, Dr. Yellin argues that President Wilson’s administration successfully segregated the federal government in the age of progressive politics. He investigates how the enactment of the segregation policy imposed a color line on American opportunity and implicated Washington in the economic limitation of African Americans for decades to com
Essentials of Dental Radiography and Radiology / Eric Whaites and Nicholas Drage.
No full textPrevious edition: Essentials of dental radiography and radiology / Eric Whaites. Fourth edition. Edinburgh ; New York : Churchill Livingstone, 2007.Includes bibliographical references (pages 461-464) and index.x, 478, [2] pages
10th C. Eric Lincoln Lecture Series, 1992
No full textPart of the 10th anniversary of the C. Eric Lincoln lecture series Dr. Love Henry Whelchel moderates a panel of scholars. The panel includes Dr. C. Eric Lincoln, Dr. Jacquelyn Grant, and Dr. John Hope Franklin. The panel discusses the life of George Washington Williams (author of History of the Negro Race in America). The discussion includes issues related to Womanist theology, Islamic religion, sociology, religion and history.The Atlanta University Center Robert W. Woodruff Library acknowledges the generous support of the National Endowment for Humanities - Humanities Collections and Reference Resources Implementation Project Grant in supporting the processing and digitization of a number of its major archival collections as part of the project: Spreading the Word: Expanding Access to African American Religious Archival Collections at the Atlanta University Center Robert W. Woodruff Library
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