1,721,325 research outputs found
Complete sequencing of an IncHI1 plasmid encoding the carbapenemase NDM-1, the ArmA 16s RNA methylase and a resistance-nodulation-cell division/multidrug efflux pump
No full textObjectives: To characterize the pNDM-CIT plasmid identified in Citrobacter freundii carrying genes encoding the metallo-β-lactamase NDM-1 and the 16S RNA methylase ArmA. Methods: The complete DNA sequence of pNDM-CIT was obtained by using the 454-Genome Sequencer FLX procedure on a library obtained using plasmid DNA purified from the pNDM-CIT Escherichia coli J53 transconjugant. Contig assembly and predicted gaps were confirmed and filled by PCR-based gap closure. Comparative analysis with IncHI1 incompatibility group plasmids was performed using BLASTN and BLASTP algorithms. Results: Plasmid pNDM-CIT was 288 920 bp and revealed an IncHI1 plasmid scaffold, showing novel resistance and potential virulence determinants. The blaNDM-1 gene was identified within a novel genetic context, flanked by a duplication of the class 1 integron on both sides. The replicase gene repAciN, originating from Acinetobacter spp. plasmids, was identified in a close association with the Tn1548::armA transposon and the macrolide resistance mel-mph2 cluster. The same structure was identified in silico from a series of enterobacterial plasmids carrying the armA gene. The repAciN gene probably represents a remnant sign of the original occurrence of the armA gene in Acinetobacter plasmids. A CP4-like prophage sequence was identified in pNDM-CIT, containing a resistance-nodulation-cell division/multidrug resistance (RND/MDR) efflux pump cluster surrounded by two IS1-like elements. This resistance determinant, associated with such a prophage sequence, has never been reported on plasmids. Conclusions: Plasmid pNDM-CIT differed significantly from all known blaNDM-1-carrying plasmids identified in Enterobacteriaceae, since it combines the metallo-β-lactamase NDM-1, the 16S RNA methylase ArmA and a cryptic prophage carrying the RND/MDR efflux pump. © The Author 2012. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved
Complete sequencing of an IncH plasmid carrying the bla ndm-1, bla ctx-m-15 and qnrB1 genes
No full textObjectives: To characterize plasmid pNDM-MAR recovered from a Klebsiella pneumoniae isolate of sequence type (ST) 15 from Morocco, carrying the genes encoding the metallo-β-lactamase NDM-1, the extended-spectrum β-lactamase (ESBL) CTX-M-15 and the qnrB1 quinolone resistance determinant. Methods: The plasmid DNA sequence was obtained by using the 454-Genome Sequencer FLX procedure on a library constructed from total plasmid DNA obtained from an Escherichia coli J53 transconjugant. Contig assembly and predicted gaps were confirmed and filled by PCR-based gap closure. Results: Plasmid pNDM-MAR was 267 242 bp long and encoded 177 predicted proteins. It harboured novel replicons and transfer loci, defining a novel plasmid type within the IncH plasmid family. The bla NDM-1 gene was flanked by genetic elements that are distinct from those observed in other bla NDM-1-positive plasmids, including the groES and groEL chaperonin genes. This plasmid harboured the ESBL gene bla CTX-M-15 together with the quinolone resistance gene qnrB1. In addition, it harboured genes encoding resistance to tellurite, mercury, chloramphenicol and aminoglycosides. Interestingly, pNDM-MAR did not carry any 16S rRNA methylase gene, in contrast to other bla NDM-1-positive plasmids. Conclusions: This study underlines the diversity of genetic vehicles involved in the spread of the bla NDM-1 gene. Plasmid pNDM-MAR differed significantly from all known bla NDM-1-bearing plasmids. Comparative analysis of the pNDM-MAR sequence identified a novel type of IncH plasmid. © The Author 2012. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved
Complete sequence of the IncT-type plasmid pT-OXA-181 carrying the bla OXA-181 carbapenemase gene from Citrobacter freundii
No full textThe gene encoding the carbapenemase OXA-181 (an OXA-48 variant) was identified from a Citrobacter freundii isolate coproducing NDM-1. The whole sequence of plasmid pT-OXA-181 bearing the blaOXA-181 gene was determined and revealed a 84-kb mobilizable but non-self-conjugative IncT-type plasmid. It totally differs from the 7.6-kb ColE-type and bla OXA-181-bearing plasmid recently identified in a Klebsiella pneumoniae isolate. However, in both plasmids, insertion sequence ISEcp1 might have played a role in acquisition of the blaOXA-181 gene. Copyright © 2013, American Society for Microbiology. All Rights Reserved
Comparative genomics of IncL/M-type plasmids: Evolution by acquisition of resistance genes and insertion sequences
No full textIn vivo development of cefiderocol resistance in carbapenem-resistant Acinetobacter baumannii associated with the downregulation of a TonB-dependent siderophore receptor, PiuA
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Spread of OXA-48-encoding plasmid in Turkey and beyond
No full textEighteen carbapenem-resistant, OXA-48-positive enterobacterial isolates recovered from Turkey, Lebanon, Egypt, France, and Belgium were analyzed. In most isolates, similar 70-kb plasmids carrying the carbapenemase gene bla OXA-48 were identified. That gene was located within either transposon Tn1999 or transposon Tn1999.2, which was always inserted within the same gene. This work highlights the current plasmid-mediated dissemination of the OXA-48 carbapenemase worldwide. Copyright © 2010, American Society for Microbiology. All Rights Reserved.Aktas Z, 2008, CHEMOTHERAPY, V54, P101, DOI 10.1159-000118661; AMUTHAN G, 1994, PLASMID, V32, P328, DOI 10.1006-plas.1994.1072; Aubert D, 2006, J BACTERIOL, V188, P6506, DOI 10.1128-JB.00375-06; Bell JM, 2009, 49 INT C ANT AG CHEM; Bonnet R, 2002, ANTIMICROB AGENTS CH, V46, P2004, DOI 10.1128-AAC.46.6.2004-2006.2002; Carattoli A, 2005, J MICROBIOL METH, V63, P219, DOI 10.1016-j.mimet.2005.03.018; Carrer A, 2008, ANTIMICROB AGENTS CH, V52, P2950, DOI 10.1128-AAC.01672-07; CASTANHEIRA M, 2009, 49 INT C ANT AG CHEM; Clinical and Laboratory Standards Institute, 2008, M100S18 CLIN LAB STA; CUZON G, 2009, 49 INT C ANT AG CHEM; Cuzon G, 2008, ANTIMICROB AGENTS CH, V52, P3463, DOI 10.1128-AAC.00543-08; Elumalai R. P., 1997, Indian Journal of Experimental Biology, V35, P408; Girlich D, 2007, APPL ENVIRON MICROB, V73, P4681, DOI 10.1128-AEM.02491-06; Gulmez D, 2008, INT J ANTIMICROB AG, V31, P523, DOI 10.1016-j.ijantimicag.2008.01.017; JARLIER V, 1988, REV INFECT DIS, V10, P867; KIESER T, 1984, PLASMID, V12, P19, DOI 10.1016-0147-619X(84)90063-5; Livermore DM, 2009, J ANTIMICROB CHEMOTH, V64, P29, DOI 10.1093-jac-dkp255; Matar GM, 2008, CLIN MICROBIOL INFEC, V14, P887, DOI 10.1111-j.1469-0691.2008.02059.x; Nazic H, 2005, ANTIMICROB AGENTS CH, V49, P2146, DOI 10.1128-AAC.49.5.2146-2147.2005; Nordmann P, 2009, LANCET INFECT DIS, V9, P228, DOI 10.1016-S1473-3099(09)70054-4; Nordmann P, 2002, CLIN MICROBIOL INFEC, V8, P321, DOI 10.1046-j.1469-0691.2002.00401.x; Poirel L, 2004, ANTIMICROB AGENTS CH, V48, P15, DOI 10.1128-AAC.48.1.15-22.2004; Poirel L, 2007, FUTURE MICROBIOL, V2, P501, DOI 10.2217-17460913.2.5.501; Poirel L, 2004, ANTIMICROB AGENTS CH, V48, P348, DOI 10.1128-AAC.48.1.348-351.2004; Poirel L, 2010, ANTIMICROB AGENTS CH, V54, P24, DOI 10.1128-AAC.01512-08; TANIMOTO K, 1985, MOL GEN GENET, V198, P356, DOI 10.1007-BF00383019; Thomas CP, 2009, 49 INT C ANT AG CHEM; TOP E, 1994, APPL ENVIRON MICROB, V60, P83111211010
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
- …
