1,720,967 research outputs found
Evidence of new sequence types of methicillin-resistant Staphylococcus pseudintermedius in Italy
No full textStaphylococcus pseudintermedius (SP) and methicillin-resistant SP (MRSP) is one of the most important veterinary pathogens in the dog. Herein, from a total of 126 S. pseudintermediusstrains, 23 MRSP (18%) were identified. Multi-Locus Sequence Typing (MLST) revealed that most of MRSP strains belonged to ST71 (26%), which have been already reported in Italy and other countries. Interestingly, nine new sequence types (39%), from 1053 up to 1061, were described for the first time. Moreover, the isolated MRSP strains showed relevant antibiotic resistance profiles. This report highlights the circulation of new sequence types of MRSP in Italy and underlines the need of a global epidemiological surveillance to limit the increasing spread of multidrug-resistant MRSPstrains worldwide, since they may represent a considerable concern for dog's health
Antibacterial and antimycotic activity of hydroxypyridinone-based iron-chelating co-polymer (DIBI) against pathogens carried by companion animals. -
No full textHydroxypyridinone-based iron-chelating co-polymer (DIBI) has antibacterial and antimycotic activity of against pathogens associated with canine skin diseases.
No full textSynergistic Effect of Abietic Acid with Oxacillin against Methicillin-Resistant Staphylococcus pseudintermedius
Full text linkResin acids are valued in traditional medicine for their antiseptic properties. Among these, abietic acid has been reported to be active against methicillin-resistant Staphylococcus aureus (MRSA) strains. In veterinary healthcare, the methicillin-resistant Staphylococcus pseudintermedius (MRSP) strain is an important reservoir of antibiotic resistance genes including mecA. The incidence of MRSP has been increasing, and treatment options in veterinary medicine are partial. Here, we investigated the antimicrobial and antibiofilm properties of abietic acid against three MRSP and two methicillin-susceptible Staphylococcus pseudintermedius (MSSP) strains, isolated from diseased pet animals and human wound samples. Abietic acid showed a significant minimal inhibitory concentration (MIC) value ranging from 32 to 64 μg/mL (MRSPs) and 8 μg/mL (MSSP). By checkerboard method we demonstrated that abietic acid increased oxacillin susceptibility of MRSP strains, thus showing a synergistic interaction with oxacillin. Abietic acid was also able to contrast the vitality of treated MSSP and MRSP1 biofilms at 20 μg/mL and 40 μg/mL, respectively. Finally, the compound moderately reduced mecA, mecR1 and mec1 gene expression. In conclusion, the results here reported demonstrate the antimicrobial activity of abietic acid against MRSP and support the use of this compound as a potential therapeutic agent to be used in combinatorial antibiotic therapy
Structural characterization and antimicrobial activity of innovative compounds: a novel strategy for in silico peptides design
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MG-132 interferes with iron cellular homeostasis and alters virulence of bovine herpesvirus 1
No full textBovine herpesvirus 1 (BoHV-1) requires an iron-replete cell host to replicate efficiently. BoHV-1 infection pro
vokes an increase in ferritin levels and a decrease of transferrin receptor 1 (TfR-1) expression, ultimately
lowering iron pool extent. Thus, cells try to limit iron availability for virus spread. It has been demonstrated that
MG-132, a proteasome inhibitor, reduces BoHV-1 release. Since ferritin, the major iron storage protein in
mammalian cells, undergoes proteasome-mediated degradation, herein, the influence of MG-132 on iron
metabolism during BoHV-1 infection was examined.
Following infection in bovine cells (MDBK), MG-132 reduced cell death and viral yield. Western blot analysis
showed a significant ferritin accumulation, likely due to the inhibition of its proteasome-mediated degradation
pathway. In addition, the concomitant down-regulation of TfR-1 expression, observed during infection, was
counteracted by proteasome inhibitor. This trend may be explained by enhanced acidic vesicular organelles,
detected by acridine orange staining, determining a reduction of intracellular pH, that promotes new synthesis of
TfR-1 degraded in a recycling pathway. In addition, MG-132 influences cellular iron distribution during BoHV-1
infection, as revealed by Perls’ Prussian blue staining. However, cellular iron content, evaluated by Atomic
Absorption Spectrophotometry, resulted essentially unaltered.
These findings reveal that MG-132 may contribute to limit cellular iron availability for virus replication
thereby enhancing cell survival
Antimicrobial activity of some essential oils against methicillin-susceptible and methicillin-resistant staphylococcus pseudintermedius-associated pyoderma in dogs
No full textGoing Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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