33 research outputs found
Detailed Key Methods
No full textDetailed key methods relating to manuscript: Olcina Monica, M., Kim Ryan, K., Balanis Nikolas, G., Li Caiyun, G., von Eyben, R., Graeber Thomas, G., Ricklin, D., Stucki, M., Giaccia Amato, J., Intracellular C4BPA levels regulate NF-κB dependent apoptosis, ISCIENCE (2020), doi: https://doi.org/10.1016/j.isci.2020.101594
Detailed Key Methods
No full textDetailed key methods relating to manuscript: Olcina Monica, M., Kim Ryan, K., Balanis Nikolas, G., Li Caiyun, G., von Eyben, R., Graeber Thomas, G., Ricklin, D., Stucki, M., Giaccia Amato, J., Intracellular C4BPA levels regulate NF-κB dependent apoptosis, ISCIENCE (2020), doi: https://doi.org/10.1016/j.isci.2020.101594
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A linear mixed model approach to gene expression-tumor aneuploidy association studies
No full textAneuploidy, defined as abnormal chromosome number or somatic DNA copy number, is a characteristic of many aggressive tumors and is thought to drive tumorigenesis. Gene expression-aneuploidy association studies have previously been conducted to explore cellular mechanisms associated with aneuploidy. However, in an observational setting, gene expression is influenced by many factors that can act as confounders between gene expression and aneuploidy, leading to spurious correlations between the two variables. These factors include known confounders such as sample purity or batch effect, as well as gene co-regulation which induces correlations between the expression of causal genes and non-causal genes. We use a linear mixed-effects model (LMM) to account for confounding effects of tumor purity and gene co-regulation on gene expression-aneuploidy associations. When applied to patient tumor data across diverse tumor types, we observe that the LMM both accounts for the impact of purity on aneuploidy measurements and identifies a new association between histone gene expression and aneuploidy
Intracellular C4BPA Levels Regulate NF-κB-Dependent Apoptosis
No full textThe importance of innate immunity in cancer is increasingly being recognized with recent reports suggesting tumor cell-intrinsic intracellular functions for innate immunity proteins. However, such functions are often poorly understood, and it is unclear whether these are affected by patient-specific mutations. Here, we show that C4b-binding protein alpha chain (C4BPA), typically thought to reside in the extracellular space, is expressed intracellularly in cancer cells, where it interacts with the NF-κB family member RelA and regulates apoptosis. Interestingly, intracellular C4BPA expression is regulated in a stress- and mutation-dependent manner and C4BPA mutations are associated with improved cancer survival outcome. Using cell lines harboring patient-specific C4BPA mutations, we show that increasing intracellular C4BPA levels correlate with sensitivity to oxaliplatin-induced apoptosis in vitro and in vivo. Mechanistically, sensitive C4BPA mutants display increased IκBα expression and increased inhibitory IκBα-RelA complex stability. These data suggest a non-canonical intracellular role for C4BPA in regulating NF-κB-dependent apoptosis
A reconfigurable PCB antenna for WiMAX, C-band and X-band operation
No full textIn this paper, a novel printed-circuit-board (PCB) antenna that employs electronic tunability is presented. The antenna is based on a 10mm x 25mm x 1.6mm FR4-epoxy substrate, is microstrip-line fed and has a partial ground plane. The proposed antenna is designed and simulated using the Finite-Element Method (FEM) and the Method of Moments (MoM) for two different switching conditions. The impedance bandwidth, gain, efficiency and radiation patterns of the antenna are demonstrated. It is shown that the antenna is suitable for WiMAX, C-band and X-band operation. © 2009 IEEE.Agilent Technologies, 2006, ADS MOM; Balanis C. A., 2005, ANTENNA THEORY ANAL; Christodoulou CG, 2007, 2007 International Workshop on Anti-counterfeiting, Security, and Identification, P9, DOI 10.1109-IWASID.2007.373684; Kingsley N, 2007, J MICROELECTROMECH S, V16, P1185, DOI 10.1109-JMEMS.2007.902462; Kumar G., 2003, BROADBAND MICROSTRIP; OGOCU G, 2005, 2005 IEEE ANT PROP B, V2, P404; Peroulis D, 2005, IEEE T ANTENN PROPAG, V53, P645, DOI 10.1109-TAP.2004.8413390
Fundamental issues in antenna design for microwave medical imaging applications
No full textThis paper surveys the development of microwave medical imaging and the fundamental challenges associated with microwave antennas design for medical imaging applications. Different microwave antennas used in medical imaging applications such as monopoles, bow-tie, vivaldi and pyramidal horn antennas are discussed. The challenges faced when the latter used in medical imaging environment are detailed. The paper provides the possible solutions for the challenges at hand and also provides insight into the modelling work which will help the microwave engineering community to understand the behaviour of the microwave antennas in coupling media
