51 research outputs found
Writers Talk Featuring Carla Buckley, Sarah Gridley, Paula McLain
No full textFeaturing Paula McLain, author of the memoir Like Family: Growing Up in Other People's Houses; poet Sarah Gridley; and Carla Buckley, author of the novel The Things that Keep us Here.The media can be accessed here: http://streaming.osu.edu/knowledgebank/cstw11/New_Voices-Carla_Buckley_Sarah_Gridley_Paula_McLain.mp3Ohio State University. Center for the Study and Teaching of Writin
The septin cytoskeleton is associated with distinct myelin structures of the central and peripheral nervous system
No full textRapid conduction of nerve impulses in the nervous system of higher vertebrates is made possible by ensheathment of nerve fibers by the specialized plasma membrane structure myelin. Consequently, failure of myelination or damage to the myelin sheath leads to severe pathology as seen in multiple sclerosis. During myelination oligodendrocytes and Schwann cells are challenged to build up and maintain a highly complex multilaminar plasma membrane structure. It is well-known that myelin membranes are divided into subdomains with distinct protein and lipid composition. Nevertheless, it is unclear how these domains are generated and then maintained throughout the adult. Especially the mechanisms of interaction between cytoskeleton elements and membrane structures in the developing and adult myelin sheath are still unknown. In this work, the interaction of the cytoskeleton protein septin(Sept6) and the myelin and lymphocyte protein MAL is demonstrated. Septins are enriched in myelin membranes which is unique for cytoskeleton elements. Most important, the loss of Sept6 in myelin is compensated by its closest homolog Sept11 which clearly points to a functional role of septins in the myelin compartment. A detailed analysis of the septin protein family in myelinating cells was performed here. It was shown that septins are coordinately regulated during differentiation and myelination in Schwann cells and oligodendrocytes. On the protein level particular septins were identified to be differentially enriched in myelin membranes. They form distinct stoechiometric complexes interacting also with actin. We propose that septin/myelin membrane complexes play an important role in myelination. Septins might be crucial in the formation and maintenance of myelin subdomains as well as in the transport of myelin components. In line with this, a possible site of interaction between Sept6 and MAL was identified in the Schwann cell cytoplasm. There, Sept6 and MAL might be important for sorting and trafficking processes crucial for the targeting of myelin components into the emerging and adult myelin sheath. Sept6-deficient mice, however, did not disclose alterations in myelin ultrastructure and protein composition besides the upregulation of Sept11. But, it is well-known that the septin cortex is very robust and that homologous isoforms might compensate for the loss of single septins. In line with this, it is shown here that myelinating Schwann cells tolerate the loss of Sept2, which might be due to such compensation mechanisms. The function of Sept7 in myelination could not be elucidated, since downregulation in in vitro myelinating cultures led to unspecific effects probably on cytokinesis and cell survival. Nevertheless, this first comprehensive study on septins in oligodendrocytes and Schwann cells generated valuable data critical for further analysis of septin function in myelin. This study provides insight into the composition of the septin cytoskeleton, its regulation during myelination and its interaction with myelin membranes. Understanding the role of the septin cytoskeleton in myelin formation and maintenance may reveal new insights in the mechanisms of myelination and remyelination in health and disease
Parental perceptions of an indoor bouldering programme for toddlers and pre-schoolers in England: an initial exploratory study
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Writing the Greek Revolution: Harry Mark Petrakis’s The Hour of the Bell
No full textPetrakis’s novels about the Greek Revolution are the most significant efforts by an American author, in this case a Greek American author, to place the narrative of the struggle in front of an American public. This paper uses the journal that Petrakis kept while writing The Hour of the Bell to examine his thoughts and motives in the composition of the book, with close attention to Petrakis’s reading of the American philhellene Samuel Gridley Howe’s contemporary history of the war. The ways Petrakis uses Howe, and the ways he does not, offer insight into Petrakis’s own goals and conflicts in writing his version of the Greek Revolution for an American public
Expansion Column Virtual Lab: Laboratory manual for liquid-solid fluidisation experiments
No full textA virtual lab (MOOC) for students chemical, civil or mechanical engineering. Students are introduced with a fluidised bed reactor (multiphase flow). This expansion column represents a liquid-solid fluidisation process applied in drinking water treatment processes. The knowledge you will gain will help you develop and improve your competence profile of a highly qualified chemical engineer.Students are informed with short lectures (films): https://doi.org/10.4121/12881009 and a manual (document). Several assignments must be completed based on recorded laboratory experiments.Complex Fluid Processin
Gridley Ethanol Demonstration Project Utilizing Gasification Technology: Feedstock Supply Plan; March 15, 2004
No full textThe ‘About Me’ Questionnaire: Factorial Structure and Measurement Invariance
No full textThe "About Me" Questionnaire (AMQ) has been used to measure components of social identity, academic self-concept and self-worth in children and adolescents in the United Kingdom and abroad. Studies have reported simple reliability statistics but a comprehensive assessment of the scale's psychometric properties has not been conducted. Confirmatory factor analysis, using a sample of 5,082 children aged 6 to 18 years from combined datasets of five cross-sectional research studies, was used to establish the psychometric soundness of the 29-item AMQ. Analysis revealed generally adequate reliability with the seven-factor structure confirmed in a replication sample. Results provide evidence of adequate psychometric properties, optimized with the omission of reverse-coded item and selected items, suggesting it is suitable for assessing social identity and academic self-concept of children and adolescents in applied settings. Tests for measurement invariance showed that the assessment of parallel constructs was strongly supported across males and females and partially supported across primary and secondary school–age groups
Measuring Parental Language to Target Families for Early-Intervention Services
No full textLack of expressive and receptive language skills can have a negative effect on a developing child if not identified and remedied early in the child’s life. Current community and individual strategies to identify families with children who may need additional support are limited, and may not be sufficient to detect child language problems before they become entrenched. The present study explores the feasibility of using observed indices of parental language as a means of identifying families whose children are at risk of poor outcomes. Fifteen-minute speech samples taken from videotaped observations of 68 English speaking Welsh parent-toddler dyads interacting in the home during free-play were coded for 11 categories of parent language. Three complex measures were developed through factor analysis; parent prompts, encouraging and critical language. Two simple language indices (parent total words and total different words) were calculated for comparison. Two complex measures evidenced acceptable levels of inter-rater reliability, reasonable stability over time (p < 0.05) and some construct validity in terms of their association with socioeconomic disadvantage. ‘Parent prompts’ predicted toddler receptive and expressive language six months later (p < 0.05). In comparison the two simple measures were more reliable and stable over time and were just as strongly predictive of toddler language. The findings suggest that observed indices of parental language could prove useful in identifying high-risk families in need of specific support, such as parent training or other speech and language support, and the use of simple measures could be integrated into the assessment frameworks used by existing Early Years services. Further research is required to establish the feasibility of integrating such methods into current service delivery and to establish the overall cost for Early Years services of incorporating this measure
Comparing Live and VideoObservation to Assess Early Parent-child Interactions in the Home
No full textObservation is the ‘gold standard’ for assessing parent-child behavior, however few studies have compared coding live, in real time, versus coding from videotapes in terms of their achievable levels of coder reliability within the field of parent programme research. This is important for practitioners and researchers for whom decisions might be influenced by time and financial constraints, but where outcomes may have real practical and clinical implications. Trained coders in the Dyadic Parent-Child Interaction Coding System Revised, coded 40 half-hour videotapes of 33 parent-toddler dyads interacting in the home on 29 items of dyadic behaviour. Four theorised composite variables were constructed. Videotaped data were compared to data drawn from the same interactions previously coded ‘live’ in the home. Correlations indicated significant agreement between the two modes at the item by item level (p < .001). Wilcoxon Rank tests revealed significant differences (p < .001) between the two modes. Eight items exceeded a ±30% change in median score suggesting clinically relevant differences. Although both methods achieved acceptable levels of inter-rater reliability, video coding achieved higher levels of agreement. Subtle differences exist between the two modes. Whilst neither mode proved superior it is suggested that they should not be used interchangeably
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