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Leituras críticas da obra de João Simões Lopes Neto: Província de São Pedro e Caderno de Sábado
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-graduação em LiteraturaEste trabalho tem como objetivo reunir e recuperar leituras críticas esparsas da obra de João Simões Lopes Neto publicadas na revista Província de São Pedro e no suplemento literário Caderno de Sábado, facilitando a pesquisa de outros estudiosos interessados em sua obra. O trabalho de transcrição foi realizado seguindo as normas padronizadas pela Filologia contemporânea, a fim de determinar os critérios adotados para a transcrição dos textos. Num primeiro momento, apresenta-se a apreciação da obra simoniana por parte dos críticos e, num segundo momento, são apresentados os vinte e oito textos selecionados para a transcrição
Cearagrylloides microcephalus Martins-Neto, n. comb.
No full textCearagrylloides microcephalus Martins-Neto, n. comb. (Figs. 2 B, E, G) 1. Cearagryllus microcephalus Martins-Neto, 1991. Specimens included. GP/ 1 T- 1680 (holotype), RGMN- 203, CV- 1699, and RGMN- 40 (510) New supplementary material. RGMN- 508. Emended diagnosis. Females with robust body, varying from 24 to 26 mm length; fore wing length varying from 24 to 28 mm; ovipositor length around 38 mm. Ovipositor 1.6 times longer than the body and/or the fore wing length. Head notably small, laterally elongated. ScP straight, not thickened; R parallel to ScP. MP unbranched; around six MP 2 secondary branches. r-m indistinct. Discussion. The specimen CV- 1699, with incompletely preserved ovipositor, was initially interpreted as belonging to the species Cearagryllus perforatorius (Martins-Neto, 1991), based just on the body and wing length. However, after re-examination of the venation of this specimen, it is now possible to verify that it belongs to Cearagrylloides microcephalus. In addition to the original characters assigned to the species (smaller body and head, longer ovipositor), the fore wing provided other important characters. In C. perforatorius ScP is sigmoid, distally divergent and basally thickened, in contrast to C. microcephalus, which exhibits a straight ScP, not thickened. Additionally, MA has typically three anterior secondary branches, not present in C. microcephalus. Another minor difference is a smaller number of MP secondary branches.Published as part of Martins-Neto, Rafael Gioia & Tassi, Lara Vaz, 2009, The Orthoptera (Ensifera) from the Santana formation (Early Cretaceous, Northeast Brazil): A statistical and paleoecological approach, with description of new taxa, pp. 21-37 in Zootaxa 2080 on page 28, DOI: 10.5281/zenodo.18734
The prodomain restricts Neto activities.
No full text<p>(A) Diagram and Western blot analysis of constitutively active (CA) and processing mutant (PM) Neto-GFP variants. (B) Fluorescence images of salivary glands from third instar larvae expressing Neto-GFP variants as indicated (<i>G14>neto-GFP</i>). Prodomain processing did not affect the apical localization of Neto. (C) Co-immunoprecipitation (IP: α-GFP, WB: α-GluRIIC) from muscle extracts from control (<i>y<sup>1</sup>w<sup>1118</sup></i>) and <i>neto</i> rescued larvae (<i>neto<sup>36</sup>;G14>CA-neto-GFP</i> and <i>neto<sup>36</sup>;G14>PM-neto-GFP</i>) showed that both CA- and PM-Neto bind iGluRs. (D-E) PM-Neto is less efficient in rescuing the embryonic lethality and adult viability of <i>neto</i> null mutants compared with control or CA-Neto. Genotypes: control (<i>neto<sup>36</sup>;G14>neto-GFP</i> at 25°C); CA-Neto normal (<i>neto<sup>36</sup>;G14>CA-neto-GFP-N4</i> at 18°C); CA-Neto high (<i>neto<sup>36</sup>;G14>CA-neto-GFP-N4</i> at 25°C); PM-Neto normal (<i>neto<sup>36</sup>;G14>PM-neto-GFP-D2</i> at 25°C); PM-Neto high (<i>neto<sup>36</sup>;G14>PM-neto-GFP-D1</i> at 25°C). The numbers of animals analyzed are indicated in each bar. Bars: 10 μm.</p
Allocearagryllus leipnitzi Martins-Neto, n. comb.
No full textAllocearagryllus leipnitzi Martins-Neto, n. comb. (Fig. 1 C) 1. Notocearagryllus leipnitzi Martins-Neto, 2002 b Etymology. In honor of the foraminiferologist Prof. Dr. Itamar Ivo Leipnitz (UNISINOS-RS). Holotype. RGMN-T029, Martins-Neto Collection, housed at the Sociedade Brasileira de Paleoartropodologia – SBPr. Type locality, type stratum and age. As for Notocearagryllus dutrae Martins-Neto. Diagnosis. As for the genus. Description (Holotype, Fig. 1 C). Male tegmen 25 mm long as preserved (around 2 / 3 of the probable total length). RP, CuA and pre-lanceolate cell partially preserved. Speculum fairly square, with the anterior margin slightly curved than the posterior one. Anterolateral margin as long as the posterolateral one. sp 1 reaches the distal part of the posterior margin and the apical part of the anterolateral margin, parallel to the anal margin, converging on sp 2; sp 2 reaches the distal part of the posterolateral margin and the apical part of the anterolateral margin, close to the sp 1 extremity. CuA secondary branches beginning above the speculum, and zigzag-shaped at their base, perpendicularly deflecting toward the apical margin, then running, slightly convergent, alongside the anal margin. Intraspecular cells numerous immediately under the speculum and also after it. Chords notably curved. The d vein partially fused to CuP, converging towards CuA. The d-am crossvein situated at the boundary of both anterior and anterolateral margins of the speculum. Apical part of d connected to the apical part of the posterolateral margin of the speculum by a long cross-vein. The area below the posterior margin of the speculum of similar width to the adjacent areas (area below the anterolateral margin of the speculum and area between the posterolateral margin of the speculum and the most proximal CuA secondary branch) and filled by parallel cross-veins. Remarks. This specimen was originally described by Martins-Neto (2002 b) under the collection number RGMN- 3000, now removed to a definitive collection number RGMN-T029 at the same Institution (SBPr).Published as part of Martins-Neto, Rafael Gioia & Tassi, Lara Vaz, 2009, The Orthoptera (Ensifera) from the Santana formation (Early Cretaceous, Northeast Brazil): A statistical and paleoecological approach, with description of new taxa, pp. 21-37 in Zootaxa 2080 on page 25, DOI: 10.5281/zenodo.18734
Neto-β is a novel Neto isoform at the <i>Drosophila</i> NMJ.
No full text<p>(A) Diagram of the <i>Drosophila neto</i> locus. Two isoforms are generated by alternative splicing, Neto-α and Neto-β. They have different cytoplasmic domains, but share highly conserved domains, CUB (complement <u>C</u>1r/C1s, <u>U</u>egf, <u>B</u>MP1), LDLa (LDL receptor class a) and transmembrane (TM), with Neto proteins from vertebrates and <i>C</i>. <i>elegans</i>. (B) Confocal images of third instar larvae NMJ4 from <i>neto</i><sup><i>null</i></sup> animals rescued with <i>neto</i>-<i>α</i> (<i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i><sup><i>α</i></sup>), <i>neto</i>-<i>β</i> (<i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i><sup>β</sup>), and a <i>neto</i> transgene lacking any intracellular part, <i>neto</i>Δ<sup>intra</sup><i>(neto</i><sup><i>36</i></sup>;<i>G14>neto</i>Δ<sup>intra</sup>). These Neto variants can rescue the viability and NMJ development in <i>neto</i><sup><i>null</i></sup> animals. The Neto-ex signals mark all NMJs, but the Neto-β antibodies specifically label control and <i>neto-β</i> rescued NMJs. (C-D) Confocal images of late embryos ventral muscle fields (indicated in white) labeled for (C) Neto-ex (red), Neto-β (green), HRP (blue) and (D) GluRIIA (red), Neto-β (green), HRP (blue) indicating the presence of Neto-β in the early stages of the larvae development. Genotypes: control (<i>w</i><sup><i>1118</i></sup>); <i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i><sup><i>α</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y; G14-Gal4/UAS-neto-A9</i>); <i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i><sup>β</sup> (<i>neto</i><sup><i>36</i></sup>, <i>UAS-neto-B6/Y; G14-Gal4/+</i>); <i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i>Δ<sup>intra</sup>(<i>neto</i><sup><i>36</i></sup><i>/Y; G14-Gal4/ UAS-neto</i>Δ<sup>intra</sup><i>-H4</i>). Scale bars: (B) 20 μm (C-D) 10 μm; 5 μm in details.</p
Neto-β, but not Neto-α, restores PAK recruitment and mEJP amplitude at <i>neto</i><sup><i>null</i></sup> NMJs.
No full text<p>(A) Confocal images of larval NMJ4 labeled for Neto-ex (red), PAK (green) and HRP (blue). Neto-β, but not Neto-α, restores PAK synaptic accumulation over a large range of concentrations tested. (B) Western blot comparison of Neto levels in muscle extracts from control (first lane), and <i>neto</i><sup><i>null</i></sup> larvae rescued with <i>neto-α</i> transgenes (low, medium, and high expression) (magenta gradient), or <i>neto-β</i> transgenes (low, medium, high, and very high expression)(blue gradient). Arrows indicate unprocessed and processed Neto-α (magenta) and Neto-β (blue). (B’)—low exposure. Tubulin was used as a loading control. (C-H) Electrophysiological recordings of <i>neto</i><sup><i>null</i></sup> NMJs rescued by various levels of Neto-α or Neto-β. Data are reported relative to controls matched by rearing at 18°C (empty bars) or 25°C (hatched bars). Representative traces of mEJPs and EJPs are shown in (C) and (F), respectively. The mEJPs amplitude is reduced at NMJs rescued by low and medium levels of Neto-α (D). The mEJPs frequency appears less dependent on Neto levels/isoforms, but is significantly increased in larvae reared at 18°C (E). The EJPs amplitudes are largely normal (G), likely due to subtle, but significant increases in quantal content at Neto-α-rescued NMJs (H). (I) Confocal images of NMJ4 boutons (segment A3) in control (<i>w</i><sup><i>1118</i></sup>) and <i>GluRIIA</i><sup><i>SP16/Df</i></sup> (<i>GluRIIA</i><sup><i>SP16</i></sup>/ <i>Df(2L)cl</i><sup><i>h4</i></sup>) third instar larvae labeled for Neto-ex (red), PAK (green), and GluRIIA (blue). PAK is normally present at <i>GluRIIA</i> mutant synapses, indicating that the synaptic recruitment PAK does not depend on GluRIIA. Genotypes: control (<i>G14-Gal4/+</i>); <i>neto</i><sup><i>α low</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-A9</i>), reared at 25°C); <i>neto</i><sup><i>α med</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-A3</i>, 18°C); <i>neto</i><sup><i>α high</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-A3</i>, 25°C); <i>neto</i><sup><i>β low</i></sup> (<i>neto</i><sup><i>36</i></sup>, <i>UAS-neto-B6/Y</i>; <i>G14-Gal4/+</i>, 18°C); <i>neto</i><sup><i>β med</i></sup> (<i>neto</i><sup><i>36</i></sup>, <i>UAS-neto-B6</i>/Y; <i>G14-Gal4/+</i>, 25°C); <i>neto</i><sup><i>β high</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-B3</i>, 18°C); <i>neto</i><sup><i>β very high</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-B3</i>, 25°C). Error bars indicate SEM. ***; p<0.001, **; p<0.005, *; p<0.05, ns; p>0.05. Scale bars: 20 μm, 2 μm in details and (I).</p
Prodomain processing affects Neto-mediated iGluR clustering.
No full text<p>(A-B) Confocal images of NMJ4 (A) and bouton details (B) in larvae of indicated genotypes labeled for Brp (green), GluRIIC (red), and Neto (blue). Similar to <i>neto</i> transgenes, <i>CA-neto</i> induced dose-dependent gain-of-function NMJ phenotypes. In contrast, <i>PM-neto</i> transgenes severely disrupted the NMJ morphology and the synaptic contacts. (C) Confocal images of NMJ4 labeled for GluRIIA (green), GluRIIB (red), and HRP (blue) uncovered a drastic reduction of GluRIIA synaptic signals at PM-Neto rescued NMJs. (D) Western blot comparison of Neto expression levels in muscle extracts from third instar larvae rescued with: (i) untagged Neto (lane 2, <i>neto<sup>36</sup>;G14>neto-A3</i>); (ii) CA-Neto-GFP, normal (lane 3) and high (lane 4); and (iii) PM-Neto-GFP, normal (lane 5) and high (lane 6). (*) uncleaved proteins. (E-F) Quantification of various synaptic signals at <i>neto</i> null NMJs rescued with normal levels of CA- and PM-Neto. (G) Bouton numbers were severely reduced at PM-Neto rescued NMJs. The numbers of NMJs examined are indicated in each bar. Genotypes: CA-Neto normal (<i>neto<sup>36</sup>;G14>CA-neto-GFP-N4</i> at 18°C); CA-Neto high (<i>neto<sup>36</sup>;G14>CA-neto-GFP-N4</i> at 25°C); PM-Neto normal (<i>neto<sup>36</sup>;G14>PM-neto-GFP-D2</i> at 25°C); PM-Neto high (<i>neto<sup>36</sup>;G14>PM-neto-GFP-D1</i> at 25°C). Error bars indicate SEM. *; <i>p</i><0.001, **; <i>p</i><0.01. Bars: 10 μm, 1 μm in details.</p
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