59 research outputs found
The impact of vasoactive and inflammatory reagents on arteriolar vasomotion in the gluteus maximus of mice.
No full textSkeletal muscle depends on its arteriole network to meet metabolic demands during physical activity. However, inflammatory mediators can disrupt intercellular communication along arterioles, impairing blood flow regulation and thus negatively impact muscle function. This project evaluated arteriole responsiveness in the gluteus maximus (a locomotory muscle) of C57/BL6 mice in vivo using intravital microscopic techniques. Arterioles were demonstrated as functional using specific endothelial and smooth muscle cell specific reagents. Inducing inflammation with platelet activating factor (PAF) gave rise to an increased degree of arteriolar vasoconstriction as its concentration increased. In contrast, vasodilation transitioned to vasoconstriction as histamine concentration increased. However, the arteriolar effects of histamine were attenuated during NO inhibition with N[superscript omega]-nitro-L-arginine methyl ester (L-NAME), illustrating a NO-related mechanism. Therefore, our study established the gluteus maximus as a viable candidate for inflammatory studies related to locomotion, so that the links between physical activity, inflammation, and microvascular health may be investigated. --P. ii.The original print copy of this thesis may be available here: http://wizard.unbc.ca/record=b162505
Quaestiones in Vetus et Novum Testamentum. Haymo Halberstatensis. Hrabanus Maurus. Fulgentius Ruspensis. Maximus Taurinensis. Augustinus Hipponensis. Halitgarius Cameracensis. Isidorus Hispalensis
No full textNumérisation effectuée à partir d'un document originalAppartient à l'ensemble documentaire : PlnskyFAAppartient à l'ensemble documentaire : PlnskyFA03Mécénat : Ce manuscrit a été numérisé dans le cadre de « France-Angleterre, 700-1200 : manuscrits médiévaux de la Bibliothèque nationale de France et de la British Library, un programme de la Fondation Polonsky ».F. 1-40v, 69-75v et 166-187v Quaestiones in Vetus et Novum Testamentum : « Ab exordio mundi... Dic michi nox... — ... Rabbi ubi habitus. Finit. » ; « Interrogandum est qui sunt qui evangelium... — ... virginitate corporum » ; « Quid est Deus ? Deus igitur... — ... animas diligamus. » (tiré d'ISIDORE DE SEVILLE, P. L. , LXXXIII, 155 et suiv.) F. 42v HAYMO AUTISSIODORENSIS Homiliae ( P. L. , CXVIII, n os CI, CII, CIV). F. 61v RABANUS MAURUS, Homilia ( P. L. , CX, n° CVIII). F. 65-66 S. BENEDICTUS, Regula, c. IV ( P. L. , CIII, 797-805, et édit. Linderbauer, 23-25). F. 66v Notes sur la prédication : « Primo omnium querendum... — ... his qui diligunt eum. » F. 67v « De significatione septem gentium quarum Israhel accepit terras... Hae sunt septem gentes... — ... concessa substancia sit. » F. 75 Explanatio de Oratione dominica : « Pater noster... Nemo fuit ante ausus... — ... copiosa est in celis. » F. 78v S. FULGENTIUS, Sermo ( P. L. , LXV, 719-726). F. 83v S. MAXIMUS TAURINENSIS, Sermo I, cum addit., Homilia III, Sermo II de tempore ( P. L. , LVII, 759, 227-230, 533-536). F. 87 S. AUGUSTINUS (?), Sermo de s. Stephano ( P. L. , XXXIX, 1684-1686). F. 89 HALITGARIUS CAMERACENSIS, De vitiis et virtutibus, lib. I, c. 1 ( P. L. , CV, 657-659). F. 90v AMBROSIUS AUTPERTUS, De conflictu vitiorum et virtutum, incompl. : « — ... collisa desertent... » ( P. L. , LXXXIII, 1131-1133). F. 92 « Expositio sancti Evangelii aedita a GREGORIO pape (sic) ... Mattheus sicut in ordine... — ... accipiunt gratiam Spiritui Sancto. » attribuée au pseudo saint Jérôme (P.L., CXIV, 861-916). F. 141 ISIDORUS HISPALENSIS, Allegoriae quaedam sacrae Scripturae ( P. L. , LXXXIII, 97-130). F. 156v « De gladio secundum Lucam. Est enim locus Evangelii... — ... formis Spiritus Sancti. » F. 161v Sermon : « Egredietur virga... Flos de virga ascendens... — ... adsistit qui vivit et regnat... » F. 187v Canon CXXV du concile d'Aix-la-Chapelle de 816 ( M. G., Concilia , II, 405)
Predicting habitat suitability for basking sharks (Cetorhinus maximus) in UK waters using ensemble ecological niche modelling
No full textThis is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.The basking shark (Cetorhinus maximus) is an endangered species in the north-east Atlantic, having been historically over exploited. Whilst near-shore aggregation hotspots in the UK have been identified, robust knowledge on species distribution and abundance outside these areas remains limited. Research techniques, such as habitat modelling, could however be used to gain a greater knowledge of the species distribution to inform management plans to aid population recovery. For large mobile species gathering wide-scale distribution data can be financially and logistically challenging. In lieu of conducting a UK-wide expensive strategic survey for basking sharks, we use data from two regional-scale surveys, which were conducted in southwest England and western Scotland, and use an Ensemble Ecological Niche Model (EENM) to produce a spatially explicit map of habitat suitability. When compared against a ~20-year database of public sightings of basking sharks across UK coastal seas (to 6 nautical miles offshore), patterns of habitat suitability yielded a statistically significant agreement with areas known to support basking shark sightings. EENMs could be used to advise Marine Protected Area (MPA) selection, as well as to inform environmental impact assessments for offshore developments. The application of EENM outputs could be wide-reaching and benefit not only basking sharks but other large mobile marine species in the north-east Atlantic
Theological controversy in the seventh century concerning activities and wills in Christ
No full textThe primary purpose of the thesis is to fill the existing gaps in our understanding of various theological and political aspects of the controversy that took place in both Eastern and Western parts of the Roman Empire in the seventh century, the main theological point of which was wether Christ had one or two energeiai and wills. Before coming to any conclusions on this subject, I shall investigate the preliminary forms of Monenergism and Monothelitism i.e., belief in a single energeia and will of Christ, which were incorporated in the major Christological systems developed by Apollinarius of Laodicea, Theodore of Mopsuestia, and Severus of Antioch (chapters 1-3).Against this background, it becomes obvious that the Chalcedonian Monenergism and later Monothelitism emerged from the movement of neo- Chalcedonianism. It was an attempt by the political and ecclesiastical authorities to achieve a theological compromise with various non-Chalcedonian groups, mainly Severian, but also 'Nestorian'. Their ultimate goal was to reconcile these groups with the Catholic Church of the Empire (chapter 4). However, this project of reconciliation on the basis of the single-energeia formula was contested by the representatives of the same neo-Chalcedonian tradition and consequently condemned at the Councils of Lateran (649) and Constantinople (680/681). Thus, the same neo-Chalcedonian tradition produced two self-sufficient and antagonistic doctrines. A major concern of the thesis is to expose and compare systematically their doctrinal content per se and in the wider context of the principles of neo-Chalcedonianism (chapter 5)
Origen de la muerte embrionaria espontánea en un modelo natural: <i>Lagostomus maximus</i> (vizcacha de llanura)
No full textEn la década del 70, Barbara Weir describió las características del sistema reproductor de la hembra de Lagostomus maximus, vizcacha de llanura, como así también ciertos eventos reproductivos y gestacionales como la poliovulación de 200-800 ovocitos/estro y la muerte embrionaria de múltiples implantaciones (las ubicadas en los segmentos craneales y medias de los cuernos uterinos) a partir de los 26 dpc. Esta autora concluyó que la muerte embrionaria no se debía a factores endógenos (diferencias morfológicas, vasculares, y superfetación) o exógenos (agentes etiológicos). A partir del año 2000 se realizaron estudios morfológicos y endocrinológicos con la finalidad de comprender con mayor profundidad dicho evento. A partir de los resultados preexistentes, se desarrolló esta tesis doctoral con el fin de investigar el origen de la muerte embrionaria espontánea en L. maximus. Para ello, se diseñó un estudio cuya hipótesis fue “existen diferencias morfológicas y moleculares en las distintas porciones de los cuernos uterinos de la vizcacha que determinan la supervivencia de aquellas implantaciones más próximas al cuello uterino”. Los resultados obtenidos a partir de la metodología planteada corroboran la hipótesis. Las similitudes en la morfología microscópica de los segmentos uterinos sin preñez en aspectos como la expresión de los glicoconjugados, los residuos glicosídicos, los marcadores de renovación celular y los receptores hormonales demuestran la capacidad del órgano en permitir la implantación en toda su extensión. Sin embargo, se observan diferencias significativas en cuanto al espesor de la pared uterina y sus túnicas, y en las áreas glandular y vascular a lo largo del eje craneocaudal que podrían relacionarse con las diferencias regionales uterinas en la posibilidad de permitir la continuidad de la preñez. Estas similitudes y diferencias se mantienen en las hembras con implantaciones tempranas, en donde además se encuentra que el sitio de implantación caudal de cada cuerno tiene un aspecto viable, lo que no ocurre en el craneal y el medio que se caracterizan por la presencia de infiltración leucocitaria, hemorragia y depósito de fibrina. Por su parte, en las hembras con gestación intermedia se observaron mayores diferencias en el estado de las implantaciones. Algunas de estas características, como la desorganización y la desintegración tisular, actuarían como DAMPs, promoviendo una respuesta inflamatoria esteril en la que hay infiltración neutrofílica, mastocitos, macrófagos, células gigantes de cuerpo extraño y fibrosis. Por otro lado, los estudios amplian las características del desarrollo prenatal de la vizcacha, siendo algunas de ellas exclusivas para los histricomorfos. Por otro lado, se describen nuevas estructuras como la mesoplacenta.
De esta manera, se concluye que los cuernos uterinos de L. maximus presentan aspectos heterogéneos en su sentido craneocaudal a diferentes niveles de organización, y que estas diferencias son, al menos parcialmente, las causantes del origen de la muerte embrionaria espontánea y sectorizada de la especie. Si bien la muerte embrionaria espontánea en un evento reproductivo conservado entre los diferentes taxones de mamíferos, en ninguno de ellos se observa la masividad y la sectorización presente en L. maximus. En consecuencia, esta especie representa un modelo no convencional de muerte embrionaria espontánea, cuyas características permiten el diseño de múltiples estudios para comprender la biología reproductiva de otras especies con este tipo de muerte, como así también enfermedades relacionadas con la preñez, principalmente, durante periodos tempranos.In the 1970s, Barbara Weir described the characteristics of the female reproductive system of Lagostomus maximus, plains viscacha, as well as certain reproductive and gestational events such as poliovulation of 200-800 oocytes / estrus and embryonic death from multiple implantations (those located in the cranial and middle segments of the uterine horns) from 26 dpc. At that time and with fewer techniques than the current ones, this author concluded that this embryonic death was not due to endogenous factors (morphological and vascular differences, and superfetation) or exogenous (etiological agents). Since 2000, morphological and endocrinological studies have been carried out in order to understand this event in greater depth. Consequently, and starting from the pre-existing results, this doctoral thesis was developed in order to investigate the origin of spontaneous embryonic death in L. maximus. So, a study was designed whose hypothesis was “there are morphological and molecular differences in the different portions of the uterine horns of the viscacha that determine the survival of those implantations closest to the cervix”. The results obtained from the proposed methodology corroborate the hypothesis. The similarities in the microscopic morphology of the uterine segments without pregnancy in aspects such as the expression of glycoconjugates, glycosidic residues, cell renewal markers, and hormone receptors demonstrate the organ's ability to allow implantation to its full extent. However, significant differences are observed in terms of the thickness of the uterine wall and its tunics, and in the glandular and vascular areas along the craniocaudal axis that could be related to regional uterine differences in the possibility of allowing pregnancy to continue. These similarities and differences are maintained in females with early implantations, where it is also found that the caudal implantation site of each horn has a viable appearance, which does not occur in the cranial and the middle, which are characterized by the presence of infiltration leukocyte, hemorrhage, and fibrin deposition. On the other hand, in females with intermediate gestation, greater differences were observed in implantation status. Some of these characteristics, such as tissue disorganization and disintegration, would act as DAMPs, promoting a sterile inflammatory response in which there is neutrophilic infiltration, mast cells, macrophages, foreign body giant cells, and fibrosis. On the other hand, the studies broaden the characteristics of the prenatal development of the viscacha, some of them being exclusive for histricomorphs. On the other hand, new structures such as the mesoplacenta are described.
In this way, it is concluded that the uterine horns of L. maximus present heterogeneous aspects in their craniocaudal axis at different levels of organization, and that these differences are, at least partially, the cause of the origin of the spontaneous and sectorized embryonic death of the species. Although spontaneous embryonic death is a conserved reproductive event among the different mammalian taxa, the massiveness and sectorization present in L. maximus is not observed in any of them. Consequently, this species represents an unconventional model of spontaneous embryonic death, whose characteristics allow the design of multiple studies to understand the reproductive biology of other species with this type of death, as well as diseases related to pregnancy, mainly during periods early.Doctor en Ciencias VeterinariasUniversidad Nacional de La PlataFacultad de Ciencias Veterinaria
Infectivity of gastropod-shed third-stage larvae of Angiostrongylus vasorum and Crenosoma vulpis to dogs
No full textBackground
Metastrongyloid parasites Angiostrongylus vasorum and Crenosoma vulpis infect wild and domestic canids and are important pathogens in dogs. Recent studies indicate that gastropod intermediate hosts infected with various metastrongyloids spontaneously shed infective third-stage larvae (L3) into the environment via feces and mucus under laboratory conditions. Shed L3 retain motility up to 120 days, but whether they retain infectivity was unknown.
Methods
To assess the infectivity of shed L3, the heart/lungs of six red foxes (Vulpes vulpes) were obtained from trappers in Newfoundland, Canada. Lungs were examined for first-stage larvae (L1) by the Baermann technique. A high number of viable A. vasorum L1 and a low number of C. vulpis L1 were recovered from one fox; these were used to infect naïve laboratory-raised Limax maximus. L3 recovered from slugs by artificial digestion were fed to two naïve purpose-bred research beagles (100 L3/dog). L1 shed by these two dogs was used to infect 546 L. maximus (2000–10,000 L1/slug). L3 shedding was induced by anesthetizing slugs in soda water and transferring them into warm (45 °C) tap water for at least 8 h. Shed L3 recovered from slugs were aliquoted on romaine lettuce in six-well tissue culture plates (80–500 L3/well) and stored at 16 °C/75% relative humidity. Four naïve research beagles were then exposed to 100 L3/dog from larvae stored for 0, 2, 4, or 8 weeks, respectively, after shedding.
Results
All four dogs began shedding C. vulpis L1 by 26–36 days post-infection (PI). All four dogs began shedding A. vasorum L1 by 50 days PI.
Conclusions
L3 infectivity for the definitive host was retained in both metastrongyloids, indicating the potential for natural infection in dogs through exposure from environmental contamination. As an additional exposure route, eating or licking plant or other material(s) contaminated with metastrongyloid L3 could dramatically increase the number of dogs at risk of infection from these parasites.University of Prince Edward IslandElanco (United States
Vibriosis in bivalves: Review of recent molecular, biochemical, and physiological studies
No full textThe emergence of new vibriosis in the European Atlantic coasts has been associated with mass mortalities of molluscs causing important economic losses. For example, Vibrio carchariae, a bacteria already isolated from shark, has been associated with a severe Haliotis epizooty in hatchery. In oysters, Crassostrea gigas, a strain closely related to Vibrio splendidus is suspected to be associated with summer mortalities. Some other pathogenic vibrios have been also isolated a few years ago: Vibrio tapetis which provokes the Brown Ring Disease in the Manila clams Ruditapes philippinarum; Vibrio splendidus which induces conchyoline deposit in Pecten maximus; and also Vibrio pectenicida which causes larval scallop mortalities in hatchery. The list is not exhaustive. Several tools have been developed to detect these pathogenic vibrios. They include serological procedures using polyclonal and monoclonal antibodies and ELISA tests, biochemical criteria and molecular methods based on the 16S or 23S rRNA nucleic acid probes hybridization. Molecular identification of V. tapetis and V. pectenicida has been recently done by dot blot hybridation using specific 16S rDNA probes and a SSP-PCR protocol method has allowed V. tapetis detection in individual diseased and asymptomatic clams. This last method suggests a potential utilization in commercial hatcheries to confirm V. tapetis free water and clams. If the virulence factors of fish pathogenic vibrios have been yet identified, little knowledge exist in mollusc pathogenic vibrios. Bivalve pathogenic vibrios are host specific, excepted V. splendidus which presents various variants depending on the host species. In this last vibrio, V. splendidus, some common mechanism mediated by molecular factors could be suspected. The interactions of the vibrios with the clam's tissus or hemocytes have been used to developed in vitro biotests to evaluate their pathogenicity. Accordind to these tests, in V. tapetis, adherence and hemocyte lysis factors have been yet identified. With this test, a toxin from Vibrio pectenicida, responsible of hemocyte lysis, was partially purified. It was a small molecule (< 3Kdal), no-proteinic different to cilio-static toxin described by Nottage and Birkbeck. This molecule is probably common at different pathogenic vibrios. In bacteria, virulence factors are generally carried by plasmids. Two or three plasmids have been yet detected in V. tapetis, but the role of these plamids in pathogenicity is still not demonstrated. In conclusion, studies of these interaction models, vibrio-bivalve, allow to develop original comparative researchs, in particular characterisation of adherence factors and toxins in vibrios but also characterisation of the immune defence mechanisms against vibrios, in particular identification of anti-bacterial substances.TR: CS0117498 'Disney's Coronado Springs Resort, January 21-25, 2001, Lake Buena Vista, Florida.'/ 'The international trienniel conference & exposition of National Shellfisheries Association, American Fisheries Society Fish Culture Section, World Aquaculture Society'--Cover./ Includes author and subject indexes
An Analysis of the Development of EFL Students’ Communicative Competence Based on Technological Knowledge in Higher Education
No full textPedagogical competency is essential in influencing the growth of EFL students within the educational context. This research aims to analyze the development of EFL students' communicative competence based on technological knowledge in higher education. This research used a descriptive qualitative research method. The numbers of respondents of this study were twenty EFL students by using stratified sample technique from ninety EFL students. The results of the study show what contexts students use to voice their opinions, ranging from talks about general topics (education policy, movies, everyday events) to talks about organizations, lecturing, in-class presentations, public presentations, and the final category, using media technologies (chats, recordings, and video recordings). The students reported feeling comfortable and motivated to engage with media technology. Pedagogical technologies might support EFL students in enhancing their communicative competence. Pedagogical technologies have the potential to motivate students to adopt a self-directed learning style and collaborate to enhance their communicative competence by providing them with access to a learning environment outside of the traditional classroom
Seawater carbonate chemistry and the mechanisms behind swimming performance of Atlantic king scallop
No full textIn the present study we therefore used a semitargeted, multi tissue NMR based metabolomic approach to analyze metabolite patterns in the Atlantic king scallop, Pecten maximus, that were long-term acclimated to different end of century conditions of ocean warming (OW), ocean acidification (OA) and their combination (OWA). We investigated tissue specific metabolic profiles and metabolite concentrations in frozen tissues from gills, mantle and phasic and tonic adductor muscle of P. maximus under present conditions using 1H-HR-MAS NMR spectroscopy.
This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were provided by the author of the related paper (see Related to) to the OA-ICC data curator. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI)
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Yvette and the Wild Shame
No full textYvette and the Wild Shame was first produced by Columbia University at The Pershing Square Signature Center in New York City on April 12, 2014. It was directed by Tasha Gordon-Solmon. The cast was as follows: Matthew McDonald - Footman, Sean McIntyre - Maximus, Becca L. McLarty - Julie, Michael Micalizzi - Wilderness Boy, Adam Thomas Smith - Footman, Jen Taher - Cheryl, Lindsay Torrey - Yvette. The lighting design was by Carolyn Wong; the costume design was by Summer Lee Jack; the sound design was by Ien DeNio; the set and props design was by Aaron Ethan Green; the music was composed by the author; the production stage manager was Michael Tosto; the stage manager was Alexandra Hall, the director of production was Joe Novak
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