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Silver-based antibacterial surfaces for bone implants
No full textTotal hip arthroplasty (THA) is the most effective and safest method for treating severe degenerative, post-traumatic and other diseases of the joints. With an aging population that is increasingly active, the use of biomedical implants will continue to rise. It is estimated that more than 1,000,000 THAs are performed each year globally. Consequently, the threat posed by implant associated infections (IAI) will affect a large percentage of this populace. IAI are serious complications, caused by infectious bacteria that colonize the implant surface, rapidly proliferate and secrete a matrix of polysaccharides known as a biofilm. Once formed, the biofilm is extremely resistant to host-defense mechanisms or antibiotics, and ultimately will lead to implant loosening and revision surgery with devastating effects on the patient. Currently, no solutions are clinically available to prevent IAI on cementless implants. Therefore, intense research efforts are focused on development of antibacterial surfaces that will kill any adherent bacteria and thus, prevent the bacteria from generating a biofilm. The aim of this PhD thesis was to develop a novel antibacterial surface for cementless implants. The research was focused on the synthesis, physicochemical characterization and biological evaluation of an Ag-based antibacterial surface on the Ti6Al7Nb biomedical alloy. Using the plasma electrolytic oxidation (PEO) process, various concentrations of Ag nanoparticles were incorporated into a microporous TiO2 layer grown on the Ti6Al7Nb alloy to render its surface antibacterial. Following synthesis, thorough characterization of the novel oxide layers with regard to Ag nanoparticles distribution, chemical and phase composition, surface roughness, coating porosity, pore density, pore size distribution, and surface free energy was performed. Furthermore, their antibacterial effectiveness and toxicity to human bone cells were assessed in vitro. Finally, a feasibility study assessing whether Ag-based antibacterial surfaces can be applied on commercially available bone implants such as plasma sprayed titanium hip components was also performed. The thesis comprises six chapters starting with an introduction in Chapter 1 that provides the background, as well as the state of the art information on implant associated infections and current biomaterial-based strategies to prevent this problem. Furthermore, a description of the PEO process, as a possible process for generating antibacterial surfaces for bone implants, and the motivation of using Ag nanoparticles as antibacterial agent are also presented. Finally, the aim of the research and the thesis outline are defined. The experimental conditions and the required equipment for the synthesis and characterization of novel Ag-based antibacterial layers on the Ti6Al7Nb alloy are presented in Chapter 2. In addition, the main results on surface characterization are reported in this chapter. During the PEO process, the Ti6Al7Nb disk was immersed in an aqueous calcium acetate/calcium glycerophosphate-based electrolyte bearing dispersed Ag nanoparticles and connected to a high-voltage power supply. A stainless steel plate served as the counter-electrode. When the applied voltage exceeded a certain critical value, dielectric breakdown of the anodic barrier TiO2 layer occurred on the surface of the Ti6Al7Nb alloy thereby resulting in a modified surface. The morphological investigations by scanning electron microscopy (SEM) revealed the presence of a porous TiO2 layer with well-separated round/elongated pores, ranging from a few nanometers up to 10 ?m in size. The presence of Ag nanoparticles in the porous TiO2 layers was confirmed by high-resolution SEM coupled with the back-scattering and energy dispersive X-ray spectroscopy (EDX) detectors. Except the presence of different concentrations of Ag nanoparticles in the TiO2 layers, the other surface characteristics such as chemical and phase composition, surface roughness, porosity, pore density, pore size distribution, mean pore size and surface free energy remained unchanged as compared with the Ag-free TiO2 layers. Therefore, the effect of Ag nanoparticles embedded in the TiO2 coatings in various concentrations, on the bacteria killing ability and viability of the bone cells could be evaluated without the interference of the other factors. The challenge addressed in Chapter 3 was the investigation of the mechanism of Ag nanoparticles incorporation in the TiO2 layers during the PEO process. How and where are the Ag nanoparticles incorporated in the porous TiO2 layer were the main research questions addressed. Thus, the Ag-bearing TiO2 layers were grown at different oxidation times i.e., 10, 30, 60, 90, 120, 180, 240 and 300 seconds and thoroughly studied in plan view and cross-section using state of the art imaging techniques such as high-resolution transmission electron microscopy and SEM coupled with EDX analyses for chemical composition. It was observed that Ag nanoparticles could be incorporated in the growing TiO2 layers starting with very initial stages of oxidation (i.e., 10 seconds) with further incorporation as the PEO process was continued for longer durations. Thorough investigation of the coatings for evidence of particles revealed three different locations of Ag within the oxide: (i) fused on the surface of the oxide (with some particles protruding from the layer), (ii) fused into the pore walls (both open and closed pores), and (iii) embedded across the thickness of the dense oxide layer (starting just above the barrier-like layer). The morphology of the Ag nanoparticles found in the layers seemed to match that of the nanoparticles used in the electrolyte. As far as the mechanism of Ag nanoparticles incorporation is concern, four main steps were proposed: (i) transport of Ag nanoparticles at the TiO2/electrolyte and Ti6Al7Nb/TiO2 interfaces by mechanical agitation and electrophoretic mobility through short-circuit channels, open pores and cracks; (ii) attachment/adsorption of Ag nanoparticles to the sites of coating growth, where under the local heating generated by the sparking events the layer is relatively soft; (iii) fusion of Ag nanoparticles into the soft oxide once the sparks extinguish and the site is cooled by the electrolyte; (iv) encroachment of the already embedded Ag nanoparticles during the coating growth as the new coating formed close to the Ti6Al7Nb/TiO2 interface might be extruded through the breakdown channels causing the molten material to fill the previously created pores and cracks moving along the Ag nanoparticles that were fused in a previous discharge event. The particular focus of Chapter 4 was to perform in vitro tests to evaluate the antibacterial activity of the TiO2 layers bearing different concentrations of Ag nanoparticles. The main challenge was to find and use the proper antibacterial assays to investigate the antibacterial activity of solid surfaces. Considering that the current available assays do not reflect the precise conditions under which the bacteria will come in close contact with a potentially bactericidal surface, new assays for surface antibacterial activity as well as leachable antibacterial activity were proposed and tested. The pathogen used for the tests was methicillin-resistant Staphylococcus aureus (MRSA), one of the most prevalent and virulent microorganism responsible for implant associated infections. Surface antibacterial activity, tested by a direct contact assay, revealed 98% and 99.75% reduction in bacteria colony forming units (CFU) for 0.3Ag and 3.0Ag surfaces, respectively. In contrast, the untreated Ti6Al7Nb and Ag-free (0Ag) surfaces showed a 1000-fold increase in bacterial CFU. Testing of leachable antibacterial activity revealed a well-defined inhibition zone around the Ag-bearing samples due to the release of Ag ions after 24 hours of incubation with MRSA. Quantification of Ag ions release and determination of total Ag content in the layers, together with the antibacterial tests, led to the conclusion that both Ag nanoparticles per se as well as Ag ions release contributed to the bactericidal activity of the TiO2 surfaces. The next step in the development of antibacterial surfaces for bone implants was to check their toxicity to human cells. Whether or not the Ag-bearing TiO2 layers were toxic to osteoblast cells was described in Chapter 5. For this, a Simian Virus 40 (SV40)-immortalized human fetal osteoblast (SV-HFO) cell line was used. Cell viability, cell morphology and spreading, and the actin cytoskeletal organization and nucleus were studied in vitro using the Alamar Blue assay, SEM and fluorescence microscopy, respectively on TiO2 surfaces bearing different concentrations of Ag nanoparticles (i.e., 0.3Ag, 0.8Ag, 1.6Ag and 3.0Ag). The results showed that the viability of osteoblast cells was strongly dependent on the concentration of Ag nanoparticles in the layers. The 3.0Ag surfaces, because of the higher concentration of Ag nanoparticles in the TiO2, were found to be toxic to osteoblast cells. In contrast, the osteoblasts viability on the 0.3Ag surfaces, assessed by Alamar Blue, SEM and fluorescence microscopy, was not inhibited after 2, 5 and 7 days of culture. The testing of the TiO2 surfaces bearing intermediate concentrations of Ag nanoparticles appeared to follow the same trend. A significantly lower number of bone cells survived on the 0.8Ag and 1.6Ag samples after 2 days of culture. Chapter 6 covers a feasibility study to see whether the PEO process, used to render the surface of a Ti6Al7Nb alloy antibacterial, can be applied on commercially available cementless bone implants such as plasma-sprayed titanium hip components. Such implants have very complex geometries and the main challenge was to create an Ag-bearing antibacterial TiO2 layer that covers accurately the topography of such implants. A titanium plasma-sprayed acetabular cup was chosen as a substrate and PEO was performed using the Ag-bearing calcium acetate/calcium glycerophosphate electrolyte. The superimposed layers were then characterized with respect to surface morphology and chemistry using SEM and EDX. The surface morphology results showed the successful creation of the TiO2 layer, with its fine network of interconnected, round or elongated pores, superimposed on the macro-porous structure of the plasma-sprayed coating. The PEO antibacterial layer followed accurately the surface topography of the plasma-sprayed sample. Furthermore, the Ag nanoparticles were found to be present on top as well as in the porous structure of TiO2 layer. The work performed in this thesis leads to the final conclusions that (i) TiO2 layers bearing Ag nanoparticles can be produced on titanium biomedical alloys and on titanium-based bone implants using the plasma electrolytic oxidation process and (ii) the resultant novel layers can be a suitable engineering solution for the clinical problem of implant associated infections of cementless bone implants.BioMechanical EngineeringMechanical, Maritime and Materials Engineerin
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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