1,721,199 research outputs found
A novel process using human sera for the production of secretome and potentially clinical grade mesenchymal stem cell for regenerative therapy / Nazmul Haque
Get PDFIntroduction: Cell-based regenerative therapies offer tremendous hope to many individuals suffering from degenerative diseases. Mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. Like MSCs, cell culture supernatants and secretomes from peripheral blood mononuclear cells (PBMCs) have also shown regenerative potential. In vitro expansion of MSCs and culture of PBMCs are critical to obtain cells and secretomes with more regenerative potential. Usually, xenogeneic sera and purified recombinant proteins supplemented media are used for in vitro culture that may cause xeno-contamination and priming of cells eventually affect the regenerative outcomes. Objectives: Firstly, to compare the ability of pooled human serum (pHS) and foetal bovine serum (FBS), as supplement for the production of MSCs with more regenerative potential. Secondly, to assess the effect of autologous human serum (AuHS) and FBS in producing secretome from PBMCs. Methods: Stem cells from human extracted deciduous teeth (SHED) was used as a source of MSCs. SHED (n=3) was cultured with either pHS or FBS supplement to compare their suitability in maintaining the regenerative potential and immunomodulatory properties during in vitro expansion. The PBMCs (n=7) were cultured with either AuHS, FBS or without any serum supplement to measure viability and differentiation. Cytokines present in the secretome (n=6) were analysed. Ingenuity Pathway Analysis (IPA) were performed to predict the up/down-regulation of biological functions related to regeneration process. Results: SHED showed the characteristics of MSCs such as plastic adherence, expression of specific cell surface markers, and trilineage differentiation. Expanded SHED (n=3) showed significantly (p<0.05) higher proliferation in pHS medium compared to FBS medium. Significantly lower proportion of flattened cells was observed in pHS medium compared to FBS medium (FBS: 7%, pHS: 3%). Furthermore, migration of SHED in pHS medium was found more directional. Presence of selected 10 paracrine factors known for their proliferation and migration potential was detected in the human sera (n=6) that were used to produce pHS, none of which were detected in FBS. SHED expanded in pHS or FBS media were able to survive in the presence of complement and immune cells. IPA predicted results
showed the suitability of pHS over FBS for the expansion of SHED to maintain their regenerative potential and immunomodulatory properties. Culture of PBMCs showed that AuHS supported viability of PBMCs until 96 hours of incubation. While with the FBS supplement, the viability of PBMCs was significantly reduced at 96 hours compared to those at 0 and 24 hours of incubation (p<0.05). A significantly higher content of EGF was detected in FBS secretome collected after 24 hours (p<0.05)
compared to AuHS or basal medium secretome. While, AuHS secretome contained significantly higher amount of HGF after 24 (p<0.05) and 96 hours (p<0.01), and VEGF-A at 24 hours (p<0.05) compared to those in FBS secretome. SDF-1A was not detected in the FBS secretomes collected after either 24 or 96 hours. Conclusions: pHS has been shown to be better at supporting SHED to maintain its self-renewal capability, homogeneity and immunomodulatory properties. Besides, AuHS seems to favour cytokine composition of the secretomes with better regenerative potential
Mechanical and microseismic data presented in: Inferring microseismic source mechanisms and in situ stresses during triaxial deformation of a North-Sea-analogue sandstone
No full textData relating to publication: Inferring microseismic source mechanisms and in situ stresses during triaxial deformation of a North-Sea-analogue sandstone Published in Advances in Geosciences by authors: Luke Griffiths 1, Jérémie Dautriat 3, Ismael Vera Rodriguez 4, Kamran Iranpour 4, Guillaume Sauvin 1, Joonsang Park 1, Joel Sarout 3, Magnus Soldal 1, Lars Grande 1, Volker Oye 4, David N. Dewhurst 3, Nazmul Haque Mondol 1+2, Jung Chan Choi 1 1 NGI - Norwegian Geotechnical Institute, Oslo, 0806, Norway2 Department of Geosciences, University of Oslo, Oslo, 0371, Norway3 CSIRO Energy, Kensington WA, 6152, Australia4 NORSAR, Kjeller, 2007, NorwayCorrespondence to: Luke Griffiths ([email protected])See README.txt for details.<br
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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