12 research outputs found
Observasi ketahanan varietas padi lokal terhadap penyakit blas (Pyricularia grisea) di rumah kaca
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KERAGAMAN DAN SUMBER GEN KETAHANAN VARIETAS PADI LOKAL TERHADAP PATOGEN Pyricularia grisea PENYEBAB PENYAKIT BLAS
Get PDF[DIVERSITY AND THE SOURCE OF RESISTANCE GENE OF LOCAL RICE VARIETIES ON THE PATHOGENIC OF Pyricularia grisea CAUSE OF BLAST DISEASE]. Local rice varieties are known to have resistance or source of genes to pests even though their productivity yield is low. The pathogen of Pyricularia grisea is a cause of blast disease, which is one of the obstacles in rice production. The research aims to characterize the resistance of local rice varieties to the pathogen of P. grisea and to evaluate the virulence level of P. grisea pathogens against local rice varieties. A total of 100 local rice varieties and check varieties are susceptible and resistant namely Kencana Bali and Situ Patenggang tested their resistance to 4 dominant pathogenic of P. grisea i.e. races 033, 073, 133 and 173. Inoculation was carried out on stages 4-5 leaves or 18-21 days after seedling in a green house. The results showed a high genetic diversity of local rice varieties against pathogenic races 033, 073, 133 and 173. Based on the response of local rice varieties resistance i.e. moderately resistant (MR), resistant (R) and susceptible (S) to pathogenic races 033, 073 , 133 and 173 obtained 45 resistance response patterns. Cere Bereum varieties which are local rice varieties from West Java and Situ Patenggang resistant check varieties have a resistant response to 4 P. grisea pathogenic races used. A number of local rice varieties also show a resistant and moderately resistant response to the four pathogenic races used include Siam 11, Pare Siriendah, Menyan, Cere Manggu and Enud-Rawa Bogo. Local rice varieties Djedah and Padi Hitam (2) are local rice varieties that have a specific response of resistant or moderately resistant to race 173. Race 133 and 173 have higher virulence rates than those of races 033 and 073 on local rice varieties. The results of this study indicate that there is a great potential for the utilization of local rice varieties, as a source of resistance genes for blast disease for the assembly of rice varieties that are resistant to blast disease.
Pathogenicity of Isolates of the Rice Blast Pathogen (Pyricularia oryzae)From Indonesia
Get PDFA total of 201 isolates ofPyricularia oryzae(the causal agent of rice blast)were collected from three rice ecosystems (upland, lowland, and swampy)in five regions of Indonesia (West Java, Lampung, South Sumatra, Kali-mantan, and Bali). Their pathogenicities were characterized based on thepatterns of reaction of 25 differential varieties (DVs) and the susceptiblecontrol Lijiangxintuanheigu (LTH), which was susceptible to all blast iso-lates. A high proportion of isolates (>80.0%) were virulent to DVs for re-sistance genesPib,Pit,Pia,Pik-s,andPi12(t), and a low proportion ofisolates (<12.9%) were virulent to DVs forPik-m,Pi1,Pik-h,Pik,Pik-p,andPi7(t). Virulence to the other DVs forPish,Pii,Pi3,Pi5(t),Pi9(t),Piz,Piz-5,Piz-t,Pita-2(two lines),Pita(two lines),Pi19(t), andPi20(t)showed intermediate frequencies from 20.0 to 80.0%. These isolates wereclassified into three cluster groups, Ia, Ib, and II, and the frequencies ofcluster groups varied between the three ecosystems and the five regions.The frequencies of cluster groups varied between ecosystems and regions,and races varied according to the ecosystems. A total of 27 standard differ-ential blast isolates (SDBIs) were selected from the 201 isolates collected.The set of 25 DVs and these 27 SDBIs will be used as a new differentialsystem for analysis of the pathogenicity of blast isolates and analysis of re-sistance genes in rice cultivars, which will contribute to building a durableprotection system against blast disease in Indonesia
The Use of Nutritional Rice Specific Resistance for Control of Pathogen Pyricularia oryzae
No full textApart from being a source of energy, rice is also a source of protein and microelements that are useful for human health, such as B vitamins, iron (Fe), zinc (Zn), anthocyanins, and folic acid. Blast disease caused by the fungus Pyricularia oryzae is an important disease in rice crops worldwide. The aim of the study was to evaluate the resistance of nutritionally specific rice lines to blast disease. The nutritionally specific rice tested were preliminary yield lines and multilocation trials. The pathogens of P. oryzae used were 4 races, namely races 173, 133, 073 and 033. Observations and analysis of resistance to blast disease were carried out based on the IRRI SES method. The results for the preliminary yield trial line showed that the B15068C-MR-1-2-7-KN-2 line showed a resistant response to 4 races of blast pathogen with a disease scale of 1 and as many as 11 special rice lines showed a resistant response or moderately resistant to 4 races, while the multilocation trial lines obtained as many as 3 special rice lines and the variety of Inpari 19 which had a resistant or moderately resistant response to 4 races of P. oryzae. These nutritionally specific rice lines can be used as gene donors for resistance or proposed as varieties that have blast disease resistance. while the multilocation trial lines obtained as many as 3 special rice lines and the variety of Inpari 19 which had a resistant or moderately resistant response to 4 races of P. oryzae. These nutritionally specific rice lines can be used as gene donors for resistance or proposed as varieties that have blast disease resistance. while the multilocation trial lines obtained as many as 3 special rice lines and the variety of Inpari 19 which had a resistant or moderately resistant response to 4 races of P. oryzae. These nutritionally specific rice lines can be used as gene donors for resistance or proposed as varieties that have blast disease resistance
Pathogenicity of isolates of the rice blast pathogen (Pyricularia oryzae) from Indonesia
No full textA total of 201 isolates of Pyricularia oryzae (the causal agent of rice blast) were collected from three rice ecosystems (upland, lowland, and swampy) in five regions of Indonesia (West Java, Lampung, South Sumatra, Kalimantan, and Bali). Their pathogenicities were characterized based on the patterns of reaction of 25 differential varieties (DVs) and the susceptible control Lijiangxintuanheigu (LTH), which was susceptible to all blast isolates. A high proportion of isolates (>80.0%) were virulent to DVs for resistance genes Pib, Pit, Pia, Pik-s, and Pi12(t), and a low proportion of isolates (<12.9%) were virulent to DVs for Pik-m, Pi1, Pik-h, Pik, Pik-p, and Pi7(t). Virulence to the other DVs for Pish, Pii, Pi3, Pi5(t), Pi9(t), Piz, Piz-5, Piz-t, Pita-2 (two lines), Pita (two lines), Pi19(t), and Pi20(t) showed intermediate frequencies from 20.0 to 80.0%. These isolates were classified into three cluster groups, Ia, Ib, and II, and the frequencies of cluster groups varied between the three ecosystems and the five regions. The frequencies of cluster groups varied between ecosystems and regions, and races varied according to the ecosystems. A total of 27 standard differential blast isolates (SDBIs) were selected from the 201 isolates collected. The set of 25 DVs and these 27 SDBIs will be used as a new differential system for analysis of the pathogenicity of blast isolates and analysis of resistance genes in rice cultivars, which will contribute to building a durable protection system against blast disease in Indonesia
Observasi Observing The Performance of Gogo Rice Breeding Lines
No full textObservation is one of the stages in the selection to obtain the expected rice lines to be tested at the yield and multi-location testing stage as candidates for breeding rice varieties. The research was carried out at the Tamanbogo Lampung Experimental Garden in the 2021 planting season (MT) using an augmented design with five replications of comparison varieties: Inpago 8. Inpago 12, Inpago 13. Situbagendit, and Situpatenggang. As many as 247 promising breeding rice lines were used as materials in this experiment with their respective advantages. Each line was planted with a 0.9 m x 5 m plot size with a spacing of 30 cm x 15 cm. Fertilizer used is 300 kg/ha NPK + 100 kg/ha Urea. Weeding is done twice, precisely at the time before the next fertilization I and II. The observations include 50% flowering age, harvest age, plant height, tiller quantity, dry-milled grain yield, and resistance to biotic and abiotic stresses. The analysis of variance showed that the genotype significantly affected plant height and productive tiller quantities. High heritability and genetic advances in plant height characters indicate that this character is a selection criterion so that selection can be made to increase these characters. The comparison varieties significantly affected the character of plant height and productive tiller quantities. From this activity, 37 breeding lines were obtained with higher yields than the comparison varieties
