1,720,971 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Mechanism for Vipp1 spiral formation, ring biogenesis and membrane repair
No full textHere, we collect an image dataset of Vipp1 polymerization dynamics on supported lipid bilayers using atomic force microscopy (AFM). A JPK NanoWizard Ultraspeed AFM (Bruker and JPK BioAFM) equipped with USC-F0.3-k0.3-10 cantilevers with spring constant of 0.3N nm−1, resonance frequency of about 300 kHz (Nanoworld), was employed for image acquisition. The AFM was operated in tapping mode with a cantilever oscillation frequency near to 150kHz. Both topographic and phase images were analysed using JPKSPM Data Processing, ImageJ, and WSxM software.Peer reviewe
Heterologous expression and in-vitro analysis of Streptococcus pneumoniae FtsEX divisome complex with peptidoglycan (PG) hydrolase PcsB and actin homologue FtsA, required for PG remodelling and cell separation
Get PDFBacterial cell division is orchestrated by the divisome complex of proteins necessary for new peptidoglycan (PG) synthesis and PG remodeling during septum formation and cell separation. These proteins have homologues in both Grampositive and Gram-negative species highlighting their fundamental biological role. The complex between FtsE and FtsX is recruited to the divisome at an early stage in mid-cell division and is required in assembling further downstream divisome proteins as well as in regulating divisome activity. Specifically, it provides a membrane anchor for an extracellular hydrolase that is required for hydrolysis of PG of old cell wall material and to enable separation of daughter cells during division.
In our heterologous expression study, we observed aberrant cell division defects in Escherichia coli (Ec) cell when subject to expression of the Streptococcus pneumoniae (Sp) FtsEX mimicking the phenotype of existing antibiotics. This phenotype can be rescued co-overexpressing SpFtsEX with its cognate peptidoglycan hydrolase; PcsB that hydrolyses Escherichia coli PG required for PG remodeling during cell separation. In this study, we have demonstrated Streptococcus pneumoniae FtsEX-FtsA and FtsEX-PcsB complexes can be isolated in-vitro using nanodiscs styrene-maleic-acid-lipid-particles (SMALP), preserving their membrane lipid environment. The protein-protein interaction studies indicate SpFtsX but not SpFtsE, interacts with the essential divisome protein SpFtsA, and PcsB successfully docks with FtsEX in the SMALP disk. Negative stain electron microscopy images and initial high resolution cryo-EM trials with these complexes indicates these tools could be prerequisite for investigating mechanistic insight about their structural-functional relationship and for further inhibitor screens for these complexes
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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