1,720,965 research outputs found
Monoclonal antibodies to antigens of anthers from a dioeciuos plant: Asparagus officinalis L.
No full textMonoclonal antibodies were raised by immunizing mice with a crude extract from anthers of mature male flowers of the dioecious plant Asparagus officinalis L. To isolate anther specific antibodies, hybridoma culture supernatants were screened by enzyme-linked immunosorbent assay (ELISA) against extracts from anthers, leaves, roots of male Asparagus plants. We were able to isolate a set of anther specific monoclonal antibodies recognizing four different epitopes, as shown by ELISA experiments, Western blotting and immunolocalization. Only one of the antibodies is periodate-sensitive, implying that the epitope it recognizes is carbohydrate in nature. Indirect immunofluorescence observations and timing experiments seem to suggest that at least three of the antigens are localized in pollen cells and are present in large amounts only at relatively late times during anther development
Chirurgia disostruttiva carotidea in anestesia loco-regionale: vantaggi, svantaggi e limiti (analisi di una esperienza personale)
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells
No full textHuman chromosomal DNA coding for single chain urokinase-type plasminogen activator (scu-PA) has been inserted into a vector and expressed in LB6 cells. The recombinant clone termed 1C9 is stable and produces scu-PA at about 0.8 mg/I/day in a bioreactor. The scu-PA produced is human by amino- and carboxy-terminal analysis and immunoblotting. The recombinant scu-PA is glycosylated and fully activatable by plasmin. © 1989
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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