1,721,294 research outputs found

    Holm Oak (Quercus ilex L.) decline in Apulia, Southern Italy, a phytopathological overview.

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    Lucchese P.G., Chiaromonte E., Salamone D., Tarasco E., Pollastro S., Faretra F., Nigro F., 2025 – Holm Oak (Quercus ilex L.) decline in Apulia, Southern Italy, a phytopathological overview. 17th International Congress of Mediterranean Phytopathological Union (MPU). CIHEAM Bari, Italy, July 6-10 202

    Characterization of Methylobacterium spp. population, endophytes of olive sapwood

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    Endophytic bacteria are of biotechnological and agronomic interest as they promote plant healthiness. Investigations on the bacterial endophytic population occurring into the xylem of healthy and Xylella fastidiosa subsp. pauca (Xfp) infected olive trees showed that under field conditions, the population level of cultivable endophytic bacteria is highly variable, being mainly affected by the host genotype, host age, and wilting severity. Among the different groups, the cultivable microbial community of olive trees, either infected or not by Xfp, are Pseudomonas, Bacillus, Lysinibacillus, Pantoea, Microbacterium, Stenotrophomonas, and Methylobacterium spp. Bacteria of the genus Methylobacterium are facultative methylotrophs, and different species are endophytes in a variety of plants. As endophyte they promote plant growth and root formation by producing phytohormones and stimulating germination, enhance plant systemic resistance, supply or mobilize nutritional elements (siderophores production), and some species have also been reported as antagonist of bacterial and fungal phytopathogens. Several studies focused on Methylobacterium spp. community, which occupy the same ecological niche of Xfp in xylematic vessels of citrus plants. Buffered Charcoal Yeast Extract Agar (BCYEA) and Methanol Mineral Salts Agar (MMSA) behaved better than other microbiological media in estimating the population of spp. in the olive sapwood, and data indicated a higher population density in Xfp-infected olive sapwood than in the healthy ones, although without any statistical significance. Species of Methylobacterium have been reported as potential biocontrol agents, but it depends on which species. M. extorquens has a synergistic action, favoring the growth of Xfp and increasing the symptoms severity on citrus; conversely, the presence of M. mesophilicum reduce the growth of Xfp and consequently the symptoms severity. Colonies are pink to light red, circular and smooth with non-pigmented after growth on MMSA, for 6 days at 30°C. The analysis of 16S rRNA gene sequence and phylogenetic data by comparison with ten reference strains, showed that isolates GR18, GR19, GR22 and GR23, obtained from Xfp-infected olive tissues, cluster both with M. fujisawaense and with M. radiotolerans. Therefore, these isolates shows few differences to clearly discriminate the species. M. radiotolerans was also isolated from citrus plants infected by Xfp in Brazil, but no information about the effects on the pathogen are available. Further research are in progress to better characterize the different Methylobacterium strains, using molecular and biochemical approaches, and evaluating in planta their activity on olive quick decline syndrome

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Disciplinare di protezione integrata.

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    In: Difesa degli agrumi. Interreg II Italia-Albania, sottomisura “Introduzione di innovazioni tecnologiche nei processi produttivi
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