1,720,955 research outputs found

    EVOLUTION OF THE MITOCHONDRIAL RIBOSOMAL-RNA IN THE ORIENTAL SPECIES SUBGROUPS OF DROSOPHILA

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    The nucleotide sequence of two segments of the mtDNA molecule of different species of Drosophila containing a portion of the large rRNA (16S) and a portion of the small rRNA (23S) has been determined. The polymerase chain reaction (PCR) technique with ''universal'' primers has been used to amplify the segments for direct sequencing. The species analysed belong to four different subgroups of the melanogaster species group. The phylogenetic relationships of the species have been reconstructed by using the neighbour-joining algorithm and the significance of the inferred phylogeny tested by bootstrap method

    Evolution of reproductive strategies and male sexual ornaments in poeciliid fishes as inferred by mitochondrial 16S rRNA gene phylogeny.

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    Poeciliid fishes have been widely used as a model system in the study of sexual selection mechanisms. In several species belonging to the genera Xiphophorus and Poecilia males show conspicuous ornaments and use courtship display to obtain female cooperation during mating. Conversely most of the remaining poeciliids are drab and achieve copulation mainly through gonopodial thrusting, a fertilization strategy that bypasses the female's consent. We sequenced a portion of the large (16S) mitochondrial ribosomal RNA gene to estimate the phylogenetic relationships among the Eve tribes of the subfamily Poeciliinae (ROSEN & BAYLEY 1963) and we used the molecular phylogeny to reconstruct the origin of male ornaments and the evolutionary history of the reproductive strategies in this family. Gambusiini were found to be the basal tribe in the subfamily Poeciliinae. In our phylogeny, Xiphophorus and Poecilia were not closely related thus suggesting that the tribe Poeciliini is not a monophyletic group. Gonopodial thrusting was probably the primitive mating strategy of the poeciliids. Sexual ornaments and elaborate courtship behaviour apparently evolved more recently in the genus Xiphophorus and independently in the genus Poecilia

    Large differences in substitutional pattern and evolutionary rate of 12S ribosomal RNA genes

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    We demonstrate using Drosophila, periodical cicadas, and hominid primates, that the molecular clock based on animal mitochondrial small-subunit (12S) rRNA genes ticks at significantly different relative rates depending on which taxa and which region of the gene are examined. Drosophila, which are commonly used as model taxa, are evolving in a highly peculiar manner with the majority of sites in the 3' half of the 12S gene apparently invariant. The analogous 3' half of the mitochondrial large-subunit rRNA gene (16S) appears to be similarly constrained. It is surprising that these regions that are already highly constrained in all animals should be even more constrained in Drosophila, especially when the Drosophila mitochondrial genome as a whole does not display a similar rate slowdown. This extreme 12S rate slowdown is not apparent in periodical cicadas or hominid primates and appears to be related to strong structural and functional constraints rather than a depressed mutation rate. Finally, the slow average rate of evolution in the third domain of Drosophila does not imply that the few variable sites lack multiple hits

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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