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Ordini molteplici della costruzione nell'architettura di Peter Märkli
No full textL’indagine che qui si presenta ha la finalità di esplicitare le domande essenziali su cui l’opera dell’architetto svizzero Peter Märkli riflette nell’ambito del rapporto tra Architettura e Costru- zione.
I caratteri costitutivi del modus operandi di questo autore così singolare nel panorama architettonico contemporaneo sono forma ed espressione.
È sostanzialmente dal reciproco contrasto tra questi due termini durante il processo progettuale che Märkli inventa le proprie opere, distanziandosi da ogni modalità preconcetta o ideologica di concepirle. Una postura sempre aperta al costante dialogo con le pratiche dell’arte e con la storia delle forme dell’architettura, dalla quale risulta complessivamente un eclettismo creativo di difficile inquadramento ed interpretazione che si manifesta nell’adozione di linguaggi che spaziano dall’evocazione del linguaggio classico alla pura astrazione.
L’opera nel suo complesso è un contributo critico fondamentale ed una posizione estremamente radicale nel dibattito architettonico contemporaneo, avvicinando questo autore ai suoi maestri d’elezione: Le Corbusier, Sigurd Lewerentz, ma soprattutto Mies van der Rohe.
Analogamente alla riflessione maturata dal maestro tedesco, Märkli afferma che l’architettura è un antico linguaggio che ha una sua grammatica, rivendicando il suo essere primariamente Baukunst, e cioè, arte del costruire.The investigation presented here aims to clarify the essential questions on which the work of the Swiss architect Peter Märkli reflects in the context of the relationship between Architecture and Construction.
The constitutive characters of the modus operandi of this author so singular in the contemporary architectural panorama are form and expression.
It is substantially from the mutual contrast between these two terms during the design process that Märkli invents his own works, distancing himself from any preconceived or ideological way of conceiving them. A posture always open to constant dialogue with the practices of art and with the history of architectural forms, from which an overall creative eclecticism emerges that is difficult to classify and interpret, which manifests itself in the adoption of languages that range from the evocation of classical language to pure abstraction.
The work as a whole is a fundamental critical contribution and an extremely radical position in the contemporary architectural debate, bringing this author closer to his chosen masters: Le Corbusier, Sigurd Lewerentz, but above all Mies van der Rohe. Similarly to the reflection matured by the German master, Märkli states that architecture is an ancient language that has its own grammar, claiming its being primarily Baukunst, that is, the art of building
Systematic encoders for generalized Gabidulin codes and the q-analogue of Cauchy matrices
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Clonaggio, espressione e caratterizzazione della porzione solubile di Anaerobic induced protein A (sAniA) di Neisseria meningitidis
Full text linkNeisseria meninigitidis è facilmente esposto allo stress nitrosoattivo durante la colonizzazione e/o durante la malattia, ma può continuare a sopravvivere in ambienti poveri di ossigeno attraverso la via di denitrificazione. E' , inoltre, in grado di contrastare l'effetto tossico dell' ossido nitrico per la presenza della nitrito reduttasi (codificata dal gene aniA) primo anello del processo di denitrificazione.
Questo studio ha, come scopo, quello di valutare la conservazione della sequenza nucleotidica e aminoacidica del gene aniA; l'espressione della proteina AniA di un gruppo di ceppi di N. meninigitidis isolati da casi di infezione meningococcica (invasivi) e da portatori (non invasivi). L' analisi della sequenza di 19 ceppi invasivi e in 4 ceppi non invasivi, ha mostrato un alto grado di somiglianza nonostante siano state trovate un certo numero di cambiamenti nucleotidici alcuni dei quali definiti codoni di stop ed altri responsabili di una perdita di attività dell' enzima. In particolare, in un ceppo invasivo (1332) è stata trovata una inserzione di 9 aminoacidi vicino al sito legante il rame di tipo I ed un cambiamento dell'istidina a leucina nel sito catalitico, in posizione 280. In due ceppi non invasivi è stata identificata una parte del gene codificante una trasposasi.
Tutti i ceppi di meningococco rappresentanti le diverse alterazioni riconosciute nel gene aniA, esprimono, tuttavia, la proteina, eccetto in quei ceppi con una proteina incompleta.
La crescita in vitro di ciascun ceppo di N. meningitidis è risultata essere paragonabile sia in presenza che in assenza di nitrito, ed il mancato consumo di nitrito, suggerisce che, nonostante la proteina venga espressa in tutti i ceppi, esiste probabilmente un difetto nel consumo di questo substrato da parte dellâ enzima AniA.
Sono state studiate le proprietà biochimiche del dominio solubile dell' enzima AniA (sAniA) del ceppo di riferimento MC58, ottenuto clonando la sequenza del gene corrispondente nel vettore di espressione batterica pET28. In particolare, l' analisi biochimica di base di sAniA ha dimostrato una chiara dipendenza dell' attività catalitica in un ambiente acido.
I risultati ci suggeriscono che la presenza del gene aniA conservato e funzionale non è indispensabile per la sopravvivenza del meningococco, nonostante questo enzima sia incluso in una via metabolica importante nel ciclo di vita di questo patogeno.Neisseria meningitidis is likely to be exposed to nitrosative stress during natural colonization in the human host and disease in general. Furthermore, N. meningitidis can live in the absence of oxygen replacing the aerobic pathway with nitrite (NO2-) based metabolism involving as a first step the activity of nitrite reductase (encoded by aniA gene). During the life cycles inside the host, for instance during mucosal invasion or during sepsis, N. meningitidis may continue to survive by the denitrificant pathway. Expression of the these genes by N. meningitidis during colonization and different phases of disease plays a crucial role in bacterial survival and thus in infection.
On the basis of this knowledge, the present study evaluates sequence conservation in the gene coding for nitrite reductase (aniA) and AniA expression from a panel of Neisseria meningitidis isolates. Sequence analysis of the coding region in 19 disease-associated and 4 carrier strains notwithstanding a high degree of sequence similarity showed a number of nucleotide changes, some of which possibly resulted in premature translation termination or function loss. In particular, in one disease-associated strain a 9-residues insertion was found to be located close to the type I Cu-site and a catalytic histidine at position 280 was mutated into a leucine. In two strains from carriers, a sequence corresponding to a portion of a transposase gene within the aniA was also found. The AniA protein was always expressed, except for these two carriers strains and for other two strains in which the presence of the premature stop codons was recognized.
The biochemical properties of the cloned soluble domain of the enzyme (sAniA) from N. meningitidis reference MC58 strain and from a clinical invasive isolate, were studied. In particular, biochemical analysis of sAniA from MC58 demonstrated a clear dependence of its catalytic activity upon acidification.
The results suggest that the presence of a conserved and functional aniA gene is not essential for meningococcal survival
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
A proof of the Etzion-Silberstein conjecture for monotone and MDS-constructible Ferrers diagrams
No full textFerrers diagram rank-metric codes were introduced by Etzion and Silberstein in 2009. In their work, they proposed a conjecture on the largest dimension of a space of matrices over a finite field whose nonzero elements are supported on a given Ferrers diagram and all have rank lower bounded by a fixed positive integer d. Since stated, the Etzion-Silberstein conjecture has been verified in a number of cases, often requiring additional constraints on the field size or on the minimum rank d in dependence of the corresponding Ferrers diagram. As of today, this conjecture still remains widely open. Using modular methods, we give a constructive proof of the Etzion-Silberstein conjecture for the class of strictly monotone Ferrers diagrams, which does not depend on the minimum rank d and holds over every finite field. In addition, we leverage on the last result to also prove the conjecture for the class of MDS-constructible Ferrers diagrams, without requiring any restriction on the field size
Microcatheter–Assisted Circumferential Trabeculotomy in Primary Congenital Glaucoma: Long-Term Clinical Outcomes
Full text linkPurpose: The purpose of this study was to report the long-term efficacy and clinical outcomes of microcatheter-assisted circumferential trabeculotomy (MCT) in children with
primary congenital glaucoma (PCG). Methods: This is a single-center retrospective study including consecutive children with PCG who underwent MCT with > two years follow up. The primary
outcome was surgical success, defined as intraocular pressure (IOP) ≤ 21 mmHg with (qualified) or without (complete) medications, measured at six months, one year, and then annually. Secondary
outcomes were visual acuity (VA), refraction, axial length (AXL), complications, reinterventions, and number of medications. Results: Twelve eyes of ten patients were included. In eight children only
one eye was affected. The mean ± standard deviation (SD) age at surgery was 6.3 ± 4.1 months. The mean postoperative follow-up was 66 ± 35 months. The mean IOP was 34.3 ± 9.6 mmHg preoperatively and 14.6 ± 2.3 mmHg postoperatively at the last visit (p < 0.001). Complete success was achieved at all time points in 10 out of 12 eyes, while 2 eyes had a qualified success. At three years of age, the mean VA of the operated eyes was 0.25 ± 0.12 logMAR, the mean spherical equivalent was −0.78 ± 1.43 diopters, and the mean AXL was 23.78 mm. Transient hyphema was the only complication observed. None of the children required additional glaucoma surgery. Conclusions:
Circumferential trabeculotomy for PCG effectively lowers the IOP at more than two years after surgery. Following this procedure, the prognosis for the visual function is good, and the refractive error is low. Postoperative complications were not significant
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