1,721,140 research outputs found
Proteomic analysis of protein expression patterns between control and PCI-24781-treated SK-N-DZ cells.
No full text<p>(A) Representative 2-DE images of the control and cells treated for 36 h with 0.5 µM PCI-24781. Total proteins (500 µg) extracted from non-treated and treated cells were subjected to pH 3–10 nonlinear IPG strips and then separated by 12% SDS-PAGE. Total protein spots were visualized by CBB staining. The approximate isoelectric points (PI) and molecular mass (kDa) is shown at bottom and left of images, respectively. (B) Confirmation of expression of prohibitin, hHR23a, RuvBL2, TRAP1 and PDCD6IP in SK-N-DZ cells. Cells were treated with or without 0.5 µM PCI-24781 and then collected for Western blotting analysis as the indicated time points. (C) The effect of PCI-24781 on expression of the five selected proteins was valuated in HS-68 cells. The same treatment was performed as in SK-N-DZ cells. GAPDH was used as loading control. PCI*: PCI-24781. (D) Densitometry from Western blotting for SK-N-DZ cells and HS-68 cells following 12, 24 and 36 h treatment. Results reflect the mean ± SD for three independent experiments. (E) Total protein (40 µg) from SK-N-DZ cells untreated or treated with PCI-24781 was detected with specific antibodies for Rad51 and Ku70. GAPDH was used as loading control. PCI*: PCI-24781.</p
RuvBL2 is involved in histone deacetylase inhibitor PCI-24781-induced cell death in SK-N-DZ neuroblastoma cells.
No full textNeuroblastoma is the second most common solid tumor diagnosed during infancy. The survival rate among children with high-risk neuroblastoma is less than 40%, highlighting the urgent needs for new treatment strategies. PCI-24781 is a novel hydroxamic acid-based histone deacetylase (HDAC) inhibitor that has high efficacy and safety for cancer treatment. However, the underlying mechanisms of PCI-24781 are not clearly elucidated in neuroblastoma cells. In the present study, we demonstrated that PCI-24781 treatment significantly inhibited tumor growth at very low doses in neuroblastoma cells SK-N-DZ, not in normal cell line HS-68. However, PCI-24781 caused the accumulation of acetylated histone H3 both in SK-N-DZ and HS-68 cell line. Treatment of SK-N-DZ with PCI-24781 also induced cell cycle arrest in G2/M phase and activated apoptosis signaling pathways via the up-regulation of DR4, p21, p53 and caspase 3. Further proteomic analysis revealed differential protein expression profiles between non-treated and PCI-24781 treated SK-N-DZ cells. Totally 42 differentially expressed proteins were identified by MALDI-TOF MS system. Western blotting confirmed the expression level of five candidate proteins including prohibitin, hHR23a, RuvBL2, TRAP1 and PDCD6IP. Selective knockdown of RuvBL2 rescued cells from PCI-24781-induced cell death, implying that RuvBL2 might play an important role in anti-tumor activity of PCI-24781 in SK-N-DZ cells. The present results provide a new insight into the potential mechanism of PCI-24781 in SK-N-DZ cell line
The differentially expressed proteins regulated by PCI-24781 in SK-N-DZ cells (Average ratio ≥1.5 and ≤0.5).
No full text<p>The differentially expressed proteins regulated by PCI-24781 in SK-N-DZ cells (Average ratio ≥1.5 and ≤0.5).</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
PCI-24781 induced G2/M cell cycle arrest and apoptosis in SK-N-DZ not HS-68 cells.
No full text<p>SK-N-DZ and HS-68 cells were treated with the increasing doses of PCI-24781 for 24 h (A and B), and different time points of 0.5 µM PCI-24781 as indicated (C and D), respectively. Cell cycle analysis was performed by propidium iodide staining method. Values are the mean of three independently experiments. Control: no treatment; PCI*: PCI-24781.</p
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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