18,095 research outputs found
A Genomic Point Mutation in the Extracellular Domain of the Thyrotropin Receptor in Patients with Graves’ Ophthalmopathy
No full textOrbital and pretibial fibroblasts are targets of autoimmune attack in Graves' ophthalmopathy (GO) and pretibial dermopathy (PTD). The fibroblast autoantigen involved in these peripheral manifestations of Graves' disease and the reason for the association of GO and PTD with hyperthyroidism are unknown. RNA encoding the full-length extracellular domain of the TSH receptor has been demonstrated in orbital and dermal fibroblasts from patients with GO and normal subjects, suggesting a possible antigenic link between fibroblasts and thyrocytes. RNA was isolated from cultured orbital, pretibial, and abdominal fibroblasts obtained from patients with severe GO (n = 22) and normal subjects (n = 5). RNA was reverse transcribed, and the resulting cDNA was amplified by the polymerase chain reaction, using primers spanning overlapping regions of the entire extracellular domain of the TSH receptor. Nucleotide sequence analysis showed an A for C substitution in the first position of codon 52 in 2 of the patients, both of whom had GO, PTD, and acropachy. Genomic DNA isolated from the 2 affected patients, and not from an additional 12 normal subjects, revealed the codon 52 mutation by direct sequencing and AciI restriction enzyme digestions. In conclusion, we have demonstrated the presence of a genomic point mutation, leading to a threonine for proline amino acid shift in the predicted peptide, in the extracellular domain of the TSH receptor in two patients with severe GO, PTD, acropachy, and high thyroid-stimulating immunoglobulin levels. RNA encoding this mutant product was demonstrated in the fibroblasts of these patients. We suggest that the TSH receptor may be an important fibroblast autoantigen in GO and PTD, and that this mutant form of the receptor may have unique immunogenic properties
Graves, Robert N., 1840-1919 : Confederate Service Record, 1903.
No full textThis service record is an account of military actions during the American Civil War by veteran Robert N. Graves, 1840-1919, dated from 1903.All descriptive lists and service records in this United Confederate (Civil War) Veterans manuscript collection believed to be based out of Robert E. Lee Camp #158 of the United Confederate Veterans (Fort Worth, Tex.).The Southwest Collection Manuscript Record can be accessed at the following URL: http://www.lib.utexas.edu/taro/ttusw/00119/tsw-00119.html1 leaf, 2 pdf pages.Regiment & Battles mentioned: Confederate States of America. Army. Texas Cavalry Regiment, 6th ; Pea Ridge, Battle of, Ark., 1862
Antigen receptor variable region repertoires expressed by T cells infiltrating thyroid, retroorbital, and pretibial tissue in Graves' disease
No full textTo date, it has remained unclear whether T cells infiltrating thyroid, retroorbital, and pretibial tissue of patients with Graves' ophthalmopathy and pretibial dermopathy represent a primary immune response that is directed against certain antigenic determinants shared among these involved tissues. To characterize these T cells at the molecular level, we compared the T cell antigen receptor (TcR) variable (V) region gene usage in thyroid, retroorbital, pretibial tissue, and peripheral blood mononuclear cells of two patients with Graves' disease, ophthalmopathy, and pretibial dermopathy. Ribonucleic acid was extracted, reverse transcribed, and amplified using the PCR and 22 V alpha and 23 V beta gene-specific oligonucleotide primers. The resulting TcR V alpha and V beta transcripts were verified by Southern hybridization analysis using TcR C region-specific, digoxigenin-labeled oligonucleotide probes. In addition, complementarity determining regions 3 and junctional regions of TcR V beta genes were sequenced. Marked similarities of intrathyroidal, retroorbital, and pretibial TcR V alpha and V beta gene repertoires were noted with respect to the degree of TcR V gene restriction and the patterns of individual V genes expressed. Sequence analysis of junctional domains of V beta families revealed oligoclonality of intrahyroidal, retroorbital, and pretibial T cells. In addition, certain conserved junctional motifs were shared by T cells derived the thyroid gland and the extrathyroidal sites. Our results suggest that in the two patients with Graves' disease and extrathyroidal manifestations studied, similar antigenic determinants may have contributed to the recruitment and oligoclonal expansion of T cells both within the thyroid gland and at the involved extrathyroidal sites
Letter from Henry S. Graves, Forest Service to Carl Hayden
No full textLetter from Henry Graves to Carl T. Hayden regarding the land ownership and grazing rights of the Havasupai
Portrait of author Jackson A. Graves, [s.d.]
No full textPhotographic portrait of author Jackson A. Graves, [s.d.]. Graves is shown from his upper torso to his head and is looking to the right. He is wearing a light-colored suit, a light shirt, and a dark tie with a square pattern. He has light-colored hair that is neatly combed and parted at left, a thick light mustache, and light eyebrows. There are large bags beneath his eyes and his ears are very big
Dangerous Descent of the Aeronauts at Rainham in Essex
No full textFirst-hand accounts of a difficult balloon descent made in Rainham by Charles Green and Richard Graves MacDonnell on August 17, 1840. An accompanying illustration shows the balloon landing in tall grass during high winds.For more information about this item, visit https://archivesspace.mit.edu/repositories/2/digital_objects/73
To Live Amongst the Dead: an Ethnographic Exploration of Mass Graves in Cambodia
No full textThis thesis uses mass graves as a lens through which to examine how people in contemporary Cambodia use the Khmer Rouge period (1975 – 1979) to reconstitute and re-imagine the world they live in. Based on sixteen months of multisited ethnographic fieldwork, this thesis will argue that the Khmer Rouge regime was a critical event (Das 1997) in Cambodian life, and as such has triggered a re-shaping of relationships between local and the national, and the national and the global, leading to new forms of social and community life and action in post Khmer Rouge Cambodia. As physical markers of violence and political instability, mass graves are inherently political and articulate these re-imaginations on the state, community, and individual level. The Cambodian state exercises and legitimates its authority by constructing modern history in reference to a narrative of liberation from the Khmer Rouge, and the ‘innocent suffering’ of Cambodia and its people, while local communities use Buddhism and animism to narrate and conceptualise the period, bringing it into expected and understandable events within Khmer Buddhist cosmology. These approaches are not necessarily in opposition to one another, but rather represent the overlapping plurality of connections with mass graves.
This thesis provides a unique exploration of social relationships to mass graves in Cambodia contributing to debates within the anthropology of politics, violence and collective memory by examining how moments of national mass violence re-shape the state and relationships within it, and how destructive periods of violence nonetheless create new fields for the imagination of the political, the religious, and the social. It also contributes to the emerging field of Cambodian ethnography that combines local considerations with wider national and geo-political discourses and how these are played out at the local level
Dangerous Descent of the Aeronauts at Rainham
No full textFirst-hand accounts of a difficult balloon descent made in Rainham by Charles Green and Richard Graves MacDonnell on August 17, 1840. An accompanying illustration shows the balloon landing in tall grass during high winds.For more information about this item, visit https://archivesspace.mit.edu/repositories/2/digital_objects/65
[New Officer "Onus Probandi"] with note from Fred J. Graves, Chief of Internal Security at Gila River
No full textTraining manual for new police officers written by Fred J. Graves, Chief of Internal Security at the Gila River incarceration camp. Includes handwritten note from Graves to Schmidt.The Willard Schmidt collection, documents some of the administrative duties of Willard Schmidt, the Chief of Internal Security for the War Relocation Authority and the Tule Lake incarceration/segregation camp. This collection contains administrative records and photos documenting the Tule Lake camp, the largest incarceration camp with a peak population of 18,789 and with the most turbulent history. In 1943, the camp was turned into a segregation center to house "disloyal" Japanese Americans relocated from other camps based on their answers to a confusing loyalty questionnaire. The camp endured martial law from November 1943- Jan 1944 after escalating protests and unrest. The hostile environment of the camp lead to many incarcerees renouncing their American citizenship upon the end of incarceration, a process which took 14 years to reverse if they did not wish to be deported to Japan
The rs1990760 polymorphism within the IFIH 1 locus is not associated with Graves' disease, Hashimoto's thyroiditis and Addison's disease
No full textBackground: Three genes have been confirmed as major joint susceptibility genes for endocrine autoimmune disease:human leukocyte antigen class II, cytotoxic T-lymphocyte antigen 4 and protein tyrosine phosphatase non-receptor type 22. Recent studies showed that a genetic variation within the interferon induced helicase domain 1 (IFIH1) locus (rs1990760 polymorphism) is an additional risk factor in type 1 diabetes and Graves' disease (GD). Methods: The aim of the present study was to investigate the role of the rs1990760 polymorphism within the IFIH1 gene in German patients with GD (n=258), Hashimoto's thyroiditis (HT,n=106), Addison's disease (AD,n=195) and healthy controls (HC,n=227) as well as in 55 GD families (165 individuals, German) and 100 HT families (300 individuals, Italian). Furthermore, the interaction between rs1990760 polymorphism with human leukocyte antigen (HLA) risk haplotype DQ2(DQA*0501-DQB*0201), the risk haplotypes DQ2/DQ8 (DQA*0301-DQB*0302) and the status of thyroglobulin antibody (TgAb), thyroid peroxidase antibody (TPOAb) and TSH receptor antibody (TRAb) in patients and families were analysed. Results:No significant differences were found between the allele and genotype frequencies for rs1990760 IFIH1 polymorphism in patients with GD, HT, AD and HC. Also no differences were observed when stratifying the IFIH1 rs1990760 polymorphism for gender, presence or absence of thyroid antibodies (GD:TRAb and HT:TPOAb/TgAb) and HLA risk haplotypes (DQ2:for GD and HT, DQ2/DQ8:for AD). Furthermore the transmission analysis in GD and HT families revealed no differences in alleles transmission for rs1990760 IFIH1 from parents with or without HLA risk haplotype DQ2 to the affected offspring. In contrast, by dividing the HT parents according to the presence or absence of thyroid Ab titers, mothers and fathers both positive for TPOAb/TgAb overtransmitted the allele A of IFIH1 rs1990760 to their HT affected offspring (61.8% vs 38.2%;p=0.05;corrected p [pc]=0.1). However, these associations did not remain statistically significant after correction of the p-values. Conclusion: In conclusion, our data suggest, no contribution from IFIH1 rs1990760 polymorphism to the pathogenesis of either Graves' disease, Hashimoto's thyroiditis or Addison's disease in our study populations. However, in order to exclude a possible influence of the studied polymorphism in specified subgroups within patients with autoimmune thyroid disease, further investigations in larger populations are needed
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