163,891 research outputs found
Rescue of myeloid lineage-committed preprogenitor cells from cytomegalovirus-infected bone marrow stroma
No full textThe effect of murine cytomegalovirus on myelopoiesis was studied in long-term bone marrow culture to find an in vitro correlate for the lethal virus interference with bone marrow reconstitution (W. Mutter, M. J. Reddehase, F. W. Busch, H.-J. Bühring, and U. H. Koszinowski, J. Exp. Med. 167:1645-1658, 1988). The in vitro generation of granulocyte-monocyte progenitors (CFU-GM) discontinued after infection of the stromal cell layer, whereas the proliferation and differentiation of CFU-GM to granulocyte-monocyte colonies remained unaffected. A protocol was established to probe the functional integrity of earlier hematopoietic cells. Pre-CFU-GM (the progenitors of the CFU-GM) could be recovered from an infected bone marrow donor culture by transfer onto an inductive recipient stromal cell layer. Thus, at least in vitro, infection of bone marrow stroma appears to be the only cause of the defect in myelopoiesis
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
Molecular genetic and physiological studies to unravel the mystery of Sphingomonas wittichii RW1 dibenzo-p-dioxin degradation
No full textDibenzofuran and dibenzo-p-dioxin are ubiquitous environmental pollutants in soil and sediment. Sphingomonas wittichii RW1 is one of a few strains known for the ability to grow on the related compounds dibenzofuran (DBF) and dibenzo-p-dioxin (DXN) as the sole source of carbon. The genes for the initial steps in the DBF catabolic pathway (ring hydroxylating dioxygenase, ring cleavage dioxygenase, and a hydrolase) which result in the formation of salicylate and a five-carbon fragment have been localized to a mega-plasmid designated pSWIT02 in RW1. Plasmids highly similar to pSWIT02 have been found in other DBF degrading Sphingomonas strains. However, despite having the pSWIT02-encoded DBF degradation pathway these other bacteria are not capable of growth on DXN. This thesis describes involvement of chromosomally encoded genes in dibenzo-p-dioxin degradation by RW1. RW1 lacking the pSWIT02 dbfB gene grows extremely slowly on DBF and accumulates the ring cleavage substrate 2,2',3-trihydroxybiphenyl. The mutant grows normally on DXN as the sole source of carbon indicating that dbfB is not necessary for the DXN catabolic pathway and suggesting involvement of other ring cleavage dioxygenases in DXN pathway. Knockout of gene SWIT3046 resulted in a strain that grows normally on DBF but that does not grow on DXN. The double knockout does not grow on either DBF or DXN. These results prove that separate ring cleavage enzymes are necessary for DBF and DXN degradation.We then examined the third enzymatic step in RW1, the hydrolase. RW1 lacking the pSWIT02 encoded gene dxnB1 or the chromosome encoded gene dxnB2 grow normally on both DBF and DXN. A double knockout of both genes does not grow on DBF but still grows on DXN. We then examined previously published transcriptomic data that showed that the SWIT0910 encoded hydrolase is up regulated during growth on DBF and DXN. A knockout of SWIT0910 grows normally on DBF but does not grow on DXN. Our results demonstrate that a chromosomally encoded hydrolase, SWIT0910, is absolutely required for growth on DXN and that two different hydrolases (chromosomally and plasmid encoded) contribute equally to growth on DBF.Genes for three biphenyl ring cleavage dioxygenases from Burkholderia xenovorans LB400, Sphingomonas yanoikuyae B1, and Pseudomonas putida F1 were moved into a mutant lacking the RW1 DBF and DXN ring cleavage genes. All three ring cleavage dioxygenases allowed the mutant RW1 to grow on DBF at different rates. Interestingly, only bphC from Burkholderia xenovorans LB400 allowed RW1 mutant to grow on DXN.Ph.D.Includes bibliographical reference
Translating Brecht : versions of "Mutter Courage und ihre Kinder" for the British stage
No full textThis study analyses five British translations of Bertolt Brecht's 'Mutter Courage und ihre Kinder'. Two of these translations were written by speakers of German, and three by well-known British playwrights with no knowledge of the source text language. Four have been produced in mainstream British theatres in the past twenty-five years. The study applies translation studies methodology to a textual analysis which focuses on the translation of techniques of linguistic "Verfremdung", as well as linguistic expression of the comedy and of the political dimension in the work. It thus closes the gap in current Brecht research in examining the importance of his idiosyncratic use of language to the translation and reception of his work in the UK. The study assesses the ways in which the translator and director are influenced by Brecht's legacy in the UK and in turn, what image of Brecht they mediate through the production on stage. To this end, the study throws light on the formation of Brecht's problematic reputation in the UK, and it also highlights the social and political circumstances in early twentieth century Germany which prompted Brecht to develop his theory of an epic theatre.
The focus on a linguistic examination allows the translator's contribution to the production process to be isolated. Together with an investigation of the reception of each performance text, this in turn facilitates a more accurate assessment of the translator and director's respective influence in the process of transforming a foreign-language text onto a local stage. The analysis also sheds light on the different approaches taken by speakers of German, and playwrights creating an English version from a literal translation. It pinpoints losses in translation and adaptation, and suggests how future versions may avoid these
Computational evidence for structural consequences of kiteplatin damage on DNA
No full textThe reaction of the potential anticancer drug
kiteplatin, cis-[PtCl2(cis-1,4-DACH)], with oligomers of
single- and double-stranded DNA ranging from 2 to 12
base pairs in length was performed as a model for DNA
interaction. The potential for conformational flexibility of
single-stranded adducts was examined with density functional
theory (DFT) and compared with data from 1H-NMR
1D and 2D spectroscopy. This indicates the presence of
multiple conformations of an adduct with d(GpG), but only
one form of the adduct with d(TGGT). The importance of
a suitable theoretical model, and in particular basis set, in
reproducing experimental data is demonstrated. The DFT
theoretical model was extended to platinated base pair step
(GG/CC), allowing a comparison to the related compounds
cisplatin and oxaliplatin. Adducts of kiteplatin with larger
fragments of double-stranded DNA, including tetramer,
octamer, and dodecamer, were studied theoretically using
hybrid quantum mechanics/molecular mechanics methods.
Structural parameters of all the base-paired models were
evaluated and binding energies calculated in gas phase and in solution; these are compared across the series and
also with the related complexes cisplatin and oxaliplatin,
thus revealing insights into how kiteplatin binds to DNA
and similarities and differences between this and related
compounds
Elasmobranchs from the Lower Triassic Sulphur Mountain Formation near Wapiti Lake (BC, Canada)
No full textMutter, Raoul J., Blanger, Keith De, Neuman, Andrew G. (2007): Elasmobranchs from the Lower Triassic Sulphur Mountain Formation near Wapiti Lake (BC, Canada). Zoological Journal of the Linnean Society 149 (3): 309-337, DOI: 10.1111/j.1096-3642.2007.00244.x, URL: https://academic.oup.com/zoolinnean/article-lookup/doi/10.1111/j.1096-3642.2007.00244.
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
Mr. Melvin J. Collier, RWWL AUC, June 2011
No full textThis video is a conversation with Mr. Melvin J. Collier. Mr. Collier talks about his book, "From Mississippi to Africa: A Journey of Discovery". Daniel Le, AUC Woodruff Library, is the interviewer
Multimedia Article. The Fear of Transgastric Cholecystectomy: Misinterpretation of the Biliary Anatomy
No full textIntroduction: Prevention of injury during cholecystectomy relies on accurate dissection of the cystic duct and artery and avoidance of major biliary and vascular structures. The advent of natural orifice translumenal surgery (NOTES) has led to a new look into the biliary anatomy, especially Calot's triangle. Here we show the clinical case of a NOTES transgastric cholecystectomy for uncomplicated cholelithiasis, in which misinterpretation of the biliary anatomy occurred.
Methods and procedure: A 5-mm port was introduced at the umbilicus to ascertain the feasibility of transgastric cholecystectomy and to ensure safe gastrotomy creation and closure. Transgastric access was obtained using a percutaneous endoscopic gastrostomy (PEG)-like technique on the anterior mid body of the stomach to pass a 12-mm gastroscope (Karl Storz, Tuttlingen, Germany). The laparoscope was switched to a grasper for gallbladder retraction. Dissection was started close to the gallbladder using the endoscope at the junction between the infundibulum and what was thought to be the cystic duct. During dissection, the size and the orientation of the cystic duct appeared to be unclear. The decision was made to switch to a laparoscopic view to reorient the dissection plane and clarify the anatomy. At laparoscopy, dissection of the triangle of Calot, although started close to the gallbladder, appeared far too low. The common bile duct had been mistaken for the cystic duct. Once the biliary anatomy was clarified, the vision was switched back to the endoscope, but an additional 2-mm grasper was introduced to improve exposure while cholecystectomy was performed in a standard fashion.
Conclusions: Specific anatomic distortions due to NOTES technique together with the lack of exposure provided by current methods of retraction tend to distort Calot's triangle by flattening it rather than opening it out. At this stage, whenever the anatomy of the biliary tract is unclear, a temporary "conversion" to a laparoscopic view, more familiar to the surgeon's eye, is recommended
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