323,379 research outputs found
Alternative internet(s): Governance by internet infrastructure
No full textFrancesca Musiani is a researcher with the French National Centre for Scientific Research (CNRS), affiliated with the Institute for Communication Sciences (ISCC). In this latest post in our series on alternative internet(s), she looks at the implications of governance by control of internet infrastructure
Fuori dai salotti. L'impegno sociale delle donne bolognesi
No full textThe paper analyzes the development of the condition of women during the Risorgimento re-reading their role as central in the construction of the national discourse, with particular attention to the situation of Bologna. Not to mention the importance of the living room, as a place of sociability but also political education, played throughout the season of the struggle of the Risorgimento, the text focuses on other areas of political and social women’s expression especially those of education: kindergartens, schools and professional schools
Pietro Bubani: un percorso di vita tra tensioni rivoluzionarie e tradizioni culturali
No full textThe biography of Pietro Bubani, botanical, fits into the frame of the revolutionary years of the Italian Risorgiment
Le donne per l'Italia. Il laboratorio bolognese
No full textLa presenza femminile sulla scena pubblica dall’età dei lumi allo Stato unitario se è poco conosciuta, nondimeno è significativa per numero e spessore di personalità. Alle donne si è voluto dedicare, nell’anno in cui è ricorso il 150° dell’Unità italiana, un convegno di studio – i cui atti sono raccolti in questo volume – capace di cogliere le diverse sfaccettature del loro protagonismo. Le donne hanno discusso, parlato e scritto e si sono impegnate in prima persona nella stagione del Risorgimento italiano, in un clima europeo di primi pensieri “emancipazionisti” cresciuti sui modelli inglesi e francesi e rafforzati dalla pubblicazione nel 1848 del Manifesto di Seneca Falls. Le donne bolognesi sono state protagoniste di primo piano nei loro salotti, nei circoli, nei teatri, sul lavoro del dibattito politico e culturale che ha preparato e realizzato l’Unità italiana. Altrettanto forte è stato il loro impegno in campo socio-educativo concretizzato attraverso realizzazioni pratiche. Tutte operarono in una società in via di lento, ma costante mutamento nelle mode e nei costumi.Bolognese women were leading actors in their living rooms, clubs, theaters, on the work of political and cultural debate that has prepared and implemented the Italian unification. Equally strong was their commitment to the socio-educational concretized through practical achievements. All worked in the process of slow but constant change in society and customs
Dynamic characterization and substrate binding of cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase-an enzyme used in bioremediation.
No full textIn recent years, techniques involving the use oforganisms to remove or neutralize pollutants from contaminatedsites have attracted great attention. The aim of bioremediationis to use naturally occurring organisms to degradedangerous substances to less toxic or non toxic molecules.The gram-negative bacterium Pandoraea pnomenusa strainB-356 (Pp) has been found to be able to transform a persistentclass of organic pollutant compounds, namely the biphenyland polychlorinated biphenyls (PCBs). A key enzyme in thePCB catabolic pathway is NAD-dependent cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (BphB), for whichthe crystal structure from Pp has been crystallized in apo-,NAD-bound and biphenyldiol-/NAD-bound forms. The substratebinding loop structure has not been completely resolvedto date in the former two bound states. Here we report theresults of the first extensive molecular dynamics simulationson the three different states of PpBphB. This allowed an indepth characterization of the mechanism of ligand uptake andbinding, including unraveling of the gating mechanism. Oursimulations give a deep insight into several dynamic featuresof the enzyme that were not captured by crystal structure
Alternative internet(s) – what are they and do they have a future?
No full textThis is the first post in a series on alternative internet(s), following a workshop on the topic at the LSE in September, organised by Mélanie Dulong de Rosnay, Francesca Musiani, Alison Powell and Panayotis Antoniadis. The authors introduce the key topics that will be covered in the series here
Evolution of Macromolecular Docking Techniques: The Case Study of Nickel and Iron Metabolism in Pathogenic Bacteria
No full textThe interaction between macromolecules is a fundamental aspect of most biological processes. The computational techniques used to study protein-protein and protein-nucleic acid interactions have evolved in the last few years because of the development of new algorithms that allow the a priori incorporation, in the docking process, of experimentally derived information, together with the possibility of accounting for the flexibility of the interacting molecules. Here we review the results and the evolution of the techniques used to study the interaction between metallo-proteins and DNA operators, all involved in the nickel and iron metabolism of pathogenic bacteria, focusing in particular on Helicobacter pylori (Hp). In the first part of the article we discuss the methods used to calculate the structure of complexes of proteins involved in the activation of the nickel-dependent enzyme urease. In the second part of the article, we concentrate on two applications of protein-DNA docking conducted on the transcription factors HpFur (ferric uptake regulator) and HpNikR (nickel regulator). In both cases we discuss the technical expedients used to take into account the conformational variability of the multi-domain proteins involved in the calculations
Urease
No full textUrease is a nickel-dependent enzyme that catalyzes the reaction of water with urea. The reaction initially produces ammonia and carbamate, which then spontaneously decomposes to yield another molecule of ammonia and hydrogen carbonate. The structure and function of ureases are described
Introduzione
No full textPresentazione del volume incentrata sui temi dell'emancipazionismo femminile attraverso l'accesso allo studio
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