296 research outputs found
Dendritic cell progenitors phagocytose particulates, including Bacillus Calmette-Guerin organisms, and sensitize mice to mycobacterial antigens in vivo
No full textInaba, K., Inaba, M., Naito, M., and Steinman, R.M. Dendritic cell progenitors phagocytose particulates, including Bacillus Calmette-Guerin organisms, and sensitize mice to mycobacterial antigens in vivo. J. Exp. Med. 178: 479-488, 1993https://digitalcommons.rockefeller.edu/historical-scientific-reports/1036/thumbnail.jp
SPECTROSCOPIC REMOTE-SENSING OF MOLECULAR CONSTITUENTS IN THE ATMOSPHERE BY LASER-RADAR TECHNIQUES BASED ON OPTICAL SCATTERING PHENOMENA
No full textH. Inaba and T. Kobayasi, Nature, 224, 170, (1969). T. Kobayasi and H. Inaba, Appl. Phys. Letters, 17, 139, (1970). H. Inaba and T. Kobayasi. Invited paper to the Sixth International Quantum Electronics Conference, Kyoto, Japan, September 1970. T. Kobayasi and H. Inaba. Proceedings of IEEE., 58, 1568, (1970).""Author Institution: Research Institute of Electrical Communication, Tohoku UniversityThe usefulness of Raman spectroscopy, which is so far recognized as a complementary technique to infrared spectroscopy, can be extended to include various new fields which are unique to itself. The laser-Raman radar scheme detecting the Raman backscattered echoes has been and confirmed experimentally by the present , which is capable of measuring remotely the number density of molecular constituents as well as their species existing not only in the ordinary but also in the polluted atmosphere. This paper wishes to report the operational performance and the experimental results, so far obtained, of our laser-Raman radar as a completely single-ended system for the chemical analysis of the real atmosphere. An alternative method, which utilizes a frequency-tunable dye laser with high repetition rate of pulsed operation is also developed to investigate the resonance spectroscopic effects of a variety of molecules and atoms contained in the lower and upper atmosphere. These effects include the fluorescence and the resonance scatterings along with the resonance Raman scattering, and are expected to improve significantly the sensitivity and the range capability for the constituent analysis of our environmental air
MEASUREMENTS OF RAMAN CROSS SECTIONS FOR VARIOUS GASES IN THE POLLUTED ATMOSPHERE BY MEANS OF PULSED LASER-RAMAN AND PULSE-GATED PHOTON COUNTING METHODS
No full text H. Inaba and T. Kobayasi, Nature 224, 170 (Oct. 1969) T. Kobayasi and H. Inaba, Appl. Phys. Letters 17, 139 (Aug. 1970); Proc. of the IEEE. 58, 1568 (1970). H. Inaba and T. Kobayasi, Paper presented to 26th Symposium on Molecular Structure and Spectroscopy N4, Columbus, Ohio, June 14-18, 1971. W. F. Murphy, W. Holzer and H. J. Bernstein, Appl. Spectroscopy 23, 211 (1969). D.G. Fouche and R.K. Chang, Appl. Phys. Letters 18, 579 (June 1971).""Author Institution: Research Institute of Electrical Communication, Tohoku UniversityFeasibility and potentiality of the laser radar system which detects the Raman-shifted backscattering, either in non-resonant or resonant nature, from chemical contents of atmospheric pollutants to identify and to monitor remotely their concentrations have been However, for the precise measurement of species concentration, the knowledge on the Raman scattering cross sections is always required. This paper presents the measured result and their comparison of the Raman cross sections of various molecules present in the atmosphere such as relative to that of as a reference gas. We employed primarily a molecular nitrogen laser with 10mW average power at 3371 {\AA}, 20 kW peak power of 10 nsec duration and a repetition rate of 50 Hz. Recently, we have succeeded in the operation of transverse excitation atmospheric (TEA) nitrogen laser at 3371 {\AA} which is applicable to the field-use and also to the laboratory experiments. The Raman scattered radiation was observed in a direction perpendicular to the linearly polarized plane of the incident laser beam. The signal was processed by the pulse-gated photon counting method synchronized with the repetitive laser pulse, which is valuable for detecting extremely weak light signals. The accuracy of our measured cross sections is within 10\% and their values were found to agree well with the pre-laser result by Murphy et and the laser result by Fouche et for several gases. It is noteworthy that band has 15 times and band has 7.2 times larger cross section than that of , due possibly to resonance Raman effect at 3371 {\AA}
Macrophages phagocytose thymic lymphocytes with productively rearranged T cell receptor α and β genes
No full text[No abstract available
Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor
No full textAntigen-presenting, major histocompatibility complex (MHC) class II-rich dendritic cells are known to arise from bone marrow. However, marrow lacks mature dendritic cells, and substantial numbers of proliferating less-mature cells have yet to be identified. The methodology for inducing dendritic cell growth that was recently described for mouse blood now has been modified to MHC class II-negative precursors in marrow. A key step is to remove the majority of nonadherent, newly formed granulocytes by gentle washes during the first 2-4 d of culture. This leaves behind proliferating clusters that are loosely attached to a more firmly adherent “stroma.” At days 4-6 the clusters can be dislodged, isolated by 1-g sedimentation, and upon recuhure, large numbers of dendritic cells are released. The latter are readily identified on the basis of their distinct cell shape, ultrastructure, and repertoire of antigens, as detected with a panel of monoclonal antibodies. The dendritic cells express high levels of MHC class II products and act as powerful accessory cells for initiating the mixed leukocyte reaction. Neither the clusters nor mature dendritic cells are generated if macrophage colony-stimulating factor rather than granulocyte/macrophage colonystimulating factor (GM-CSF) is applied. Therefore, GM-CSF generates all three lineages of myeloid cells (granulocytes, macrophages, and dendritic cells). Since \u3c5 × 10 6 dendritic cells develop in 1 wk from precursors within the large hind limb bones of a single animal, marrow progenitors can act as a major source of dendritic cells. This feature should prove useful for future molecular and clinical studies of this otherwise trace cell type
Dendritic cell progenitors phagocytose particulates, including bacillus calmette-guerin organisms, and sensitize mice to mycobacterial antigens in vivo
No full textDendritic cells, while effective in sensitizing T cells to several different antigens, show little or no phagocytic activity. To the extent that endocytosis is required for antigen processing and presentation, it is not evident how dendritic cells would present particle-associated peptides. Evidence has now been obtained showing that progenitors to dendritic cells can internalize particles, including Bacillus Calmette-Guerin (BCG) mycobacteria. The particulates are applied for 20 h to bone marrow cultures that have been stimulated with granulocyte/macrophage colony-stimulating factor (GM-CSF) to induce aggregates of growing dendritic cells. Cells within these aggregates are clearly phagocytic. If the developing cultures are exposed to particles, washed, and “chased” for 2 d, the number of major histocompatibility complex class II-rich dendritic cells increases substantially and at least 50% contain internalized mycobacteria or latex particles. The mycobacterialaden, newly developed dendritic cells are much more potent in presenting antigens to primed T cells than corresponding cultures of mature dendritic cells that are exposed to a pulse of organisms. A similar situation exists when the BCG-charged dendritic cells are injected into the footpad or blood stream of naive mice. Those dendritic cells that have phagocytosed organisms induce the strongest T cell responses to mycobacterial antigens in draining lymph node and spleen. The administration of antigens to GM-CSF-induced, developing dendritic cells (by increasing both antigen uptake and cell numbers) will facilitate the use of these antigen-presenting cells for active immunization in sit
Granulocytes, macrophages, and dendritic cells arise from a common major histocompatibility complex class II-negative progenitor in mouse bone marrow
No full textThe developmental origin of dendritic cells, a specialized system of major histocompatibility complex (MHC) class II-rich antigen-presenting cells for T-cell immunity and tolerance, is not well characterized. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is known to stimulate dendritic cells, including growth and development from MHC class II-negative precursors in suspension cultures of mouse bone marrow. Here we studied colony formation in semi-solid methylcellulose cultures, a classical bioassay system in which GM-CSF induces the formation of mixed granulocyte-macrophage colonies. When colonies were induced from MHC class II-negative precursors, a small subset (1-2%) of typical dendritic cells developed alongside macrophages and granulocytes. The dendritic cells were distinguished by their cytologic features, high levels of MHC class II products, and distinct intracellular granule antigens. By using differential adherence to plastic, enriched populations of the various myeloid cell types were isolated from colonies. Only the dendritic cells stimulated a primary T-cell immune response, the mixed leukocyte reaction, and the potency was comparable to typical dendritic cells isolated from spleen. Macrophages from mixed or pure colonies were inactive as stimulator cells. Therefore, three distinct pathways of myeloid development - granulocytes, macrophages, and dendritic cells - can develop from a common MHC class II-negative progenitor under the aegis of GM-CSF
Ethics for Humanity That May Not Perish
No full textThis work is an English translation of the book “Metsubousurukamosirenai Junrui no tameno Rinrigaku[Ethics for Humanity That May Perish: LongTermism, Transhumanism, and Space Exploration]” by author, Shin-ichiro Inaba, himself. The original Japanese version was written for introducing Longtermism, Existential Risk Theory and related topics to Japanese general readrers. Chapters One and Two trace the origins of longtermism as a theoretical framework and philosophy. Chapter 3 briefly summarizes the theoretical core of what longtermism is and introduces accelerationism and effective accelerationism as adjacent movements that also concern humanity in the super-future. Chapter 4 points out that animal ethics can become a stumbling block for longtermism—giving rise to a peculiar puzzle. Chapters Five and Six examine the difficult problems in intergenerational ethics raised by Parfit, keeping the context of longtermism in mind. Chapter Five focuses on the "repugnant conclusion," while Chapter Six centers on the "non-identity problem." Chapter 7 points out that the "Singleton", according to Bostrom, deemed essential for humanity to survive and develop long-term by overcoming "existential risks," could itself be a source of such risks. Chapter 8 introduces the "Fermi Paradox" – the puzzle of why, despite the vast number of celestial bodies in the universe, extraterrestrial intelligence (ETI) remains undiscovered – and demonstrates its deep structural and substantive connection to the theory of existential risks. Chapters 9 and 10 discuss the possibility and desirability of humanity's space exploration. In closing this book, we will briefly touch upon the "eschatological" aspects of these arguments
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