4 research outputs found
Detection of Salmonella typhimurium ATCC 14028 in Powder Prepared Traditional Medicines Using Real-Time PCR
No full textThe detection of Salmonella typhimurium ATCC 14028 using real-time PCR on powdered traditional medicinal products was carried out in the microbiology and molecular biology testing laboratory of the Food and Drug Administration in Gorontalo. This research aims to provide a reference for alternative testing methods in testing the products of traditional powder preparations on the market. The sample consisted of 10 traditional powder preparations spiked with positive control of S. typhimurium ATCC 14028 phase 2. The method used in the study was real-time PCR analysis using the SYBR® Green method, while DNA isolation using the direct PCR method. Data analysis was performed by analyzing the sample's melting temperature (Tm) curve and comparing it with positive control. The results showed that S. typhimurium ATCC 14028 was detected in samples at an average Tm value of 84.18°C, with ranges of 84.0-84.5°C. For positive control, the Tm value was at 85.2°C, while for the negative control, the Tm value was not detected. Based on these data, it can be concluded that S. typhimurium ATCC 14028 in traditional medicine products powder preparations can be detected using real-time PCR
Analisis kepentingan-kinerja di Instalasi farmasi Rumah Sakit PKU Muhammadiyah Yogyakarta :: Menurut persepsi pelanggan
No full textUse of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples
No full textThe use of direct PCR technique without DNA extraction in the confirmation test for Salmonella typhimurium ATCC 14028 bacteria on meatball samples was carried out in the Food and Drug molecular biology testing laboratory Administration in Gorontalo. The basis of this research is to have an impact on economic value in carrying out the confirmation test for S. typhimurium ATCC 14028, where testing is carried out conventionally, namely DNA extraction, which requires a large amount of money. Hence, it is necessary to innovate to modify the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the direct PCR technique without DNA extraction can be done for the confirmation test of S. typhimurium ATCC 14028 on meatball samples. This study's sample consisted of 20 types of meatball samples spiked with S. typhimurium ATCC 14028 cultures. The method used in this study was qPCR analysis using the SYBR Green method. Data analysis was carried out based on 2 main criteria: (1) Ct analysis and (2) Tm analysis. Real-time PCR analysis results obtained Ct values in the range 14.14 - 15.20 with an average of 14.82 and Tm values 85.20 - 86.30 with an average of 85.79. Based on these data, it can be concluded that using direct PCR can be used for testing confirmation of S. typhimurium ATCC 14028 on meatball samples
OPTIMIZATION OF DIMETHYL SULFOXIDE AS AN ENHANCER ON EX VIVO PENETRATION OF SESEWANUA (CLERODENDRUM FRAGRANS WILD) LEAF EXTRACTS EMULGEL
No full textObjective: The present study aims to investigate the effect of using DMSO as an enhancer on the ex vivo penetration of an emulgel from sesewanua leaf extracts.
Methods: The sesewanua emulgel was prepared into four formulas, SWE1-SWE4, with different DMSO concentrations: 0%, 3%, 5%, 7%, and compared with QCE, quercetin without DMSO. Further, the penetration test of the sesewanua leaf ethanol extract emulgel was performed by determining the rate and cumulative penetration of quercetin within the skin of the mice by employing the Franz diffusion cell approach.
Results: The SWE4 emulgel containing 7% of DMSO was the best formula that enhanced the penetration of the emulgel, with a cumulative penetration at 331.423 mg/cm2, the penetration rate at 2.762 µg/cm2/minute, and better dispersibility of 4.6 cm and the results of the one-way ANOVA suggested a significant influence (p<0.05). DMSO with a concentration of 7% of the sesewanua emulgel was proven to increase 2,4-fold the rate and cumulative amount penetration. DMSO can be considered as a penetration enhancer of natural compounds for anti-inflammatory treatment.
Conclusion: The use of natural ingredients as topical anti-inflammatory continues to be developed to avoid the first-pass effects. The used of DMSO in topical emulgel preparation become a simple way to enhance the penetrant of active inggredient
