14 research outputs found
Prediction of tool deflection using image processing in ball-end milling
No full textIn milling, some of the factors that contribute to the poor quality of products are the cutting forces. Depending on the machining parameters, the cutting forces may significantly affect the tool being used in the machining process. Tool deflection can be modeled as bending deformation. Tool deflection causes poor surface quality, geometrical and dimensional errors. For this reason, it must be addressed during milling and reduced by changing the machining parameters. In the determination of tool deflection, force-based analytical and finite element methods (FEM) and sensor measurement methods are widely used. These technologies have drawbacks such as not being able to obtain fast data, being expensive, demanding precise control, and requiring continual calibration. This study aims to determine the deflection of the tool by image processing dependent on the tool/material pair and machining parameters in the milling process. For this purpose, the AL7075 material with a free-form surface was machined on a CNC milling machine. A mathematical equation is proposed to estimate the tool deflection based on the image processing results. The method has shown that tool deviation can be detected more quickly and simply by image processing.Inonu University BAP [FDK-2020-2046]The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported by Inonu University BAP (Project number FDK-2020-2046)
Injectable chondrocyte
No full textAbstract
Joint cartilage injury which affects many children and adults, involves the highly differantiated mature joint cartilage characterized by the lack of a vascular blood supply and little regeneration capacity. A newly developed method in the repair of osteochondral injuries on the femoro-tibial joint surface is autologous chondrocyte transplantation. Cells isolated from the patient can be proliferated in vitro and these cultured autologous chondrocytes can be transplanted into the defect site for the repair of the injured cartilage. In this study, we isolated the chondrocytes taken from tissue samples of human knee joint hyalin cartilage, proliferated those chondrocytes by using two different methods; a) tripsinization, b) scraping, and turned them into the injectable forms that can be used in autologous chondrocyte transplantation
Özet
Bir çok insanı etkileyen eklem hasarındaki temel sorun, yüksek oranda diferansiye olmuş, vasküler kan desteği eksik ve yalnızca sınırlı rejenerasyon kabiliyetine sahip olan erişkin eklem kıkırdağıdır. Femorotibial eklem yüzeyindeki osteokondral defektlerinin tamirinde son zamanlarda ilginin arttığı çok ümit verici bir yöntem ise orolog kondrosit transplantasyonudur. Hastanın kendisinden izole edilmiş hücreler, invitro olarak çoğaltılabilmekte ve elde edilen kültüre otolog kondrosiller, hastanın kaşarlanmış kıkırdağının tamiri için tekrar defektli bölgeye insplante edilmektedir. Biz bu çalışmamızda insan diz eklemi hiyalin kıkırdağından aldığımız doku örneklerinden izole ettiğimiz kondrositleri, monolayer kültür sistemi kullanarak çoğalttıktan sonra iki ayrı yöntem kullanarak (tripsinizasyon ve kazıma) otolog kondrosit transplantasyonunda kullanıla-cak enjektabıl forma getirdik
A new approach to chromosomal abnormalities in sperm from patients with oligoasthenoteratozoospermia: detection of double aneuploidy in addition to single aneuploidy and diploidy by five-color fluorescence in situ hybridization using one probe set
No full textObjective: To determine the frequencies of disomy, nullisomy, total aneuploidy, and diploidy in the sperms of infertile men. Design: A controlled prospective study. Setting: Assisted reproductive technology (ART)/IVF Unit and Department of Medical Biology and Genetics, Meram Medical Faculty, Konya, Turkey. Patient(s): Infertile men with oligoasthenoteratozoospermia (OAT) and normal fertile donors. Intervention(s): After slide preparation from semen samples, sperm nuclei were analyzed for chromosomes 13, 18, 21, X, and Y by five-color fluorescence in situ hybridization. Main Outcome Measure(3): The sperm aneuploidy (disomy and nullisomy) and diploidy rates were determined according to the number of signals detected for each probe in infertile and fertile men. Results: Patients with OAT had a significantly higher incidence of disomy (except chromosome 18 and XX disomy), nullisomy (except chromosome 18), and diploidy than normal fertile controls. In addition to double disomy, double nullisomy and disomy+nullisomy were observed in patients with OAT, but none of these were seen in controls. Conclusion(s): In this study patients with OAT had an increased rate of sperm aneuploidy and diploidy. This finding suggest that patients with OAT may be at an increased risk of producing aneuploid and triploid offsprings. For this reason, it may be very, important to perform the sperm fluorescence in situ hybridization in patients with OAT. Thus, a more informative genetic counseling might be given to couples with male factor infertility who are at an increased risk of having Aneuploid offsprings and triploid conceptions before intracytoplasmic sperm injection (ICSI). (Fertil Steril (R) 2008;89:1709-17. (c) 2008 by American Society for Reproductive Medicine.)
SINGLE CENTER AND SINGLE SURGEON EXPERIENCE AND THE RESULTS OF THE SURGICAL TREATMENT OF PILONIDAL SINUS
No full textAim: Pilonidal sinus is a disease deriving from hair follicules in the sacrococcygeal region. The aim of this study is to evaluate the results of patients with pilonidal sinus disease who underwent primary excision by single surgeon at single center. Material and methods: Between June 2007 and June 2010, 82 patients were enrolled in this study who operated at Ergani State Hospital. The age, sex, surgical techniques, anesthesia techniques, the time to return work, and the time of wound healing, complications and recurrences data were collected retrospectively. All patients underwent primary excision and left with open wound. Results: Between June 2007 and June 2010, 82 patients underwent primary excision and left with open wound at the same center by single surgeon. Twenty-three of patients were women (28%) and 59 were men (72%), the mean age was 23.5 (1438) years. Acute, chronic and recurrent diseases were detected in 17 (20.7%), 58 (70.8%) and 7 (8.5%) patients, respectively. Mean time to return work was 7 days (1-10), 4 patients were readmitted to hospital due to bleeding. Mean time of healing was 3 weeks (2-8). Conclusion: Primary excision with open wound is the most commonly used surgical technique in pilonidal sinus disease. It is contraversal which technique was the best in pilonidal sinus. As a result of the author experience, primary excision is safe and has the low recurrence risk
Mediterranean fever (MEFV) gene mutation frequency is not increased in adults with rheumatic heart disease
No full textIt is well established that there are people with higher risk of developing acute rheumatic fever (ARF) and rheumatic heart disease (RHD). Mediterranean fever (MEFV) gene mutations might be one of the genetic predisposition factors in the development of ARF/RHD since defect in familial Mediterranean fever (FMF) patients is proposed to be heightened inflammatory response to certain stimuli. Previous clinical observations suggested a relationship between FMF and ARF/RHD. The aim of this study was to investigate the role of the MEFV gene mutations in the susceptibility to RHD in Turkish patients. A total of 100 patients with RHD and 100 healthy controls were included in the study. Diagnosis of RHD was based on echocardiographic findings in which a predominant mitral stenosis was used as an inclusion criterion. Genetic analysis was carried out by sequence analysis investigating two hot spots (exons 2 and 10) for MEFV mutations. Mutation analysis showed that 22 RHD patients (22%) and 24 healthy controls (24%) carried at least one mutated allele. MEFV mutations were identified in 22 of 200 (11%) chromosomes in RHD patients while 26 of the 200 (13%) chromosomes of healthy controls were found to carry a mutated allele. No difference was found in allele frequencies and their distribution between the patients and healthy controls (p = 0.54). MEFV mutations are not associated with a predisposition to develop RHD in adult Turkish patients
Gorlin–chaudhry–moss syndrome revisited: Expanding the phenotype
No full textGorlin-Chaudhry-Moss syndrome (OMIM 233500) is a rare congenital malformation syndrome with the cardinal manifestations of craniofacial dysostosis, hypertrichosis, underdeveloped genitalia, ocular, and dental anomalies. Since 1960, only six affected individuals have been reported. We report a 4-year and 6-month-old female patient with this phenotype and review the clinical presentation of all patients known so far. Previously unreported malformations of the extremities, larynx, and nose are also described, expanding the phenotype of this rare syndrome. Array-CGH analysis did not show pathological deletions or duplications. (c) 2013 Wiley Periodicals, Inc
Detection of TET2, KRAS and CBL variants by Next Generation Sequencing and analysis of their correlation with JAK2 and FLT3 in childhood AML
No full textAbstractBackgroundAcute myeloid leukemia (AML) is a heterogeneous clonal disorder in terms of cytogenetic and molecular aberrations. Ten-Eleven-Translocation 2 (TET2), Kirsten rat sarcoma viral oncogene homolog (KRAS), and Casitas B-cell lymphoma (CBL) have an important role pathogenesis of acute myeloid leukemia (AML) and their activated mutations confer proliferative and survival signals.AimIn this study, we aimed to find possible genetic markers for molecular analysis in childhood AML by screening hot-spot exons of TET2, KRAS, and CBL using Next Generation Sequencing (NGS) analysis. In addition, association between found variants and mutations of Januse Kinase-2 (JAK2) and Fms-Related Tyrosine Kinase (FLT3) were analyzed which are important prognostic risk factors for AML.MethodsEight patients who were diagnosed with pediatric AML at Losante Pediatric Hematology–Oncology Hospital were included to the study. Hot-spot exons of TET2, KRAS and CBL genes were screened using the NGS method. Furthermore, FLT3-Internal Tandem Duplicate (FLT3-ITD) and JAK2-V617F were analyzed by Real Time Polymerase chain Reaction (Real Time-PCR).ResultsIn total, we identified 20 variants in studied genes by NGS. In our patient group, 16 variants in the TET2 (seven novel, seven missense and two silent), two variants in the KRAS (one missense and one intronic) and two variants in the CBL (two novel) were found. All of AML patients were found negative for JAK V617 F. Three of the eight patients (37.5%) showed mutations of both FLT3-ITD and TET2, KRAS, CBL.ConclusionWe found novel mutations for TET2, KRAS, and CBL. The detected variants in this article seem to be the first screening results of genes studied by NGS in childhood AML patients. Our results also showed some degree of association between FLT3-ITD and TET2, KRAS, CBL mutations
Thrombophilia‐associated gene mutations in women with pregnancies complicated by fetal neural tube defects
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