1,767 research outputs found
Can you picture this? Academic research published as a graphic novel!
No full textWhat would be a novel way of engaging people more people with research findings? Academics are wondering if traditional journal articles are still fit for purpose or if an injection of creativity is needed. Gareth Morris looks at how illustrated findings can draw greater impact for researchers
Diffusional Attenuation During Soft Pulses: a Zangger-Sterk Pure Shift iDOSY Experiment
No full textDiffusion-ordered spectroscopy experiments in which existing delays in a parent pulse sequence are used for diffusion encoding – iDOSY experiments – are potentially attractive because of their simplicity and sensitivity. However the calculation of diffusional attenuation in Zangger-Sterk pure shift iDOSY experiments is a very difficult problem to attack analytically, and is more easily approached numerically. Numerical simulations show that for typical experimental conditions, the dependence of diffusional attenuation on diffusion-encoding gradient amplitude is well represented by a shifted Gaussian function. The shift in gradient can be calculated analytically for the limiting case where the selective pulse is replaced by a hard 180° pulse at its midpoint; numerical simulations show that the effect of using different shapes of selective pulse is to scale down this limiting gradient shift by a constant factor that depends on the pulse shape used. The practical consequence is that under the experimental conditions appropriate for small molecules, the pure shift iDOSY method should allow good diffusion coefficient measurements to be made if appropriate allowance is made for the change in effective diffusion-encoding gradient. Parallel sets of numerical simulations and experiments are presented, and a practical application of a Zangger-Sterk pure shift iDOSY experiment to a simple test mixture is illustrated
The construction of Karen Karnak: The multi-author-function
No full textThis thesis is situated within the comparatively recent developments of Web 2.0 and the emergence of interactive WikiMedia, and explores the mode of authorship within a Read/Write culture compared to that of a Read/Only tradition. The hypothesis of this study is that the role of the audience has become merged with the author, and as such, represents new functions and attributes, distinct from a more conventional concept of authorship, in which the roles of audience and author are more separate. Read/Write and participatory culture, as defined by this study, is focused on collaboration, and includes the influences of D.I.Y. culture, Open-Source practices and the production of text by multiple authors. Multi-authorship presents a re-thinking of several concepts which support the notion of the individual author, since the focus of multi-authorship is not on attribution and ownership of a finished text, but on the continued malleability of a text. Modes of multi-authorship, demonstrated in the use of the pseudonyms Alan Smithee and Karen Eliot, represent declarative authors whose names signify multiple origins, whilst concurrently indicating a distinct body of work. The function of these names form an important context to this study, since primary research involves the construction of an experimental mode of multi-authorship utilising WikiMedia technology and the interaction of thirty nine participants, who are invited to create a body of work under the collective pseudonym Karen Karnak. The data generated by this experiment is analysed using aspects of Michel Foucault's author-function to identify and determine power structures inherent in the WikiMedia context. The interplay of power structures, including concepts such as identity, ownership and the body of work, affect the resulting mode of authorship and contribute to the construction of Karen Karnak, suggesting further areas of research into the emerging multi-author
Diffusional Attenuation During Soft Pulses: a Zangger-Sterk Pure Shift iDOSY Experiment
No full textDiffusion-ordered spectroscopy experiments in which existing delays in a parent pulse sequence are used for diffusion encoding – iDOSY experiments – are potentially attractive because of their simplicity and sensitivity. However the calculation of diffusional attenuation in Zangger-Sterk pure shift iDOSY experiments is a very difficult problem to attack analytically, and is more easily approached numerically. Numerical simulations show that for typical experimental conditions, the dependence of diffusional attenuation on diffusion-encoding gradient amplitude is well represented by a shifted Gaussian function. The shift in gradient can be calculated analytically for the limiting case where the selective pulse is replaced by a hard 180° pulse at its midpoint; numerical simulations show that the effect of using different shapes of selective pulse is to scale down this limiting gradient shift by a constant factor that depends on the pulse shape used. The practical consequence is that under the experimental conditions appropriate for small molecules, the pure shift iDOSY method should allow good diffusion coefficient measurements to be made if appropriate allowance is made for the change in effective diffusion-encoding gradient. Parallel sets of numerical simulations and experiments are presented, and a practical application of a Zangger-Sterk pure shift iDOSY experiment to a simple test mixture is illustrated.Files provided:Data====Figs. 2 to 4 and S1 to S4Figure 6a Oneshot-45Figure 6b ZS-iDOSYCode====Mathematica codeMGC analysis and figures.nbProcess simulated / experimental data for figs. 2 to 5 and S1 to S4Matlab codegradient_shift_analysis.mgradient_shift_test.m idosyzs.mSimulate data for figs. 2 to 4 and S1 to S4Sequence codegmmgcFig. 1c, for figs. 2 to 4 and S1 to S4kp_ifZS-iDOSY_03Fig. 1a, for fig. 6b ZS-iDOSYkp_oneshot45_02Fig. 1d, for fig. 6a Oneshot-45N.B. The definition of d20 has been corrected to match the diffusion delay in these sequences, correcting errors in the sequences used for acquisition of, and found in, the data directories “Figs. 2 to 4 and S1 to S4” and “Figure 6b ZS-iDOSY”VnmrJ macrosGMgshiftCalculate and apply gradient value shiftGMPKBrukerNUGCalculate and apply amplitude and diffusion coefficient correction for gradient non-uniformityGMprocPK22Produce Fig. 6aGMprocPK1002Produce Fig. 6
Diffusional Attenuation During Soft Pulses: a Zangger-Sterk Pure Shift iDOSY Experiment
No full textDiffusion-ordered spectroscopy experiments in which existing delays in a parent pulse sequence are used for diffusion encoding – iDOSY experiments – are potentially attractive because of their simplicity and sensitivity. However the calculation of diffusional attenuation in Zangger-Sterk pure shift iDOSY experiments is a very difficult problem to attack analytically, and is more easily approached numerically. Numerical simulations show that for typical experimental conditions, the dependence of diffusional attenuation on diffusion-encoding gradient amplitude is well represented by a shifted Gaussian function. The shift in gradient can be calculated analytically for the limiting case where the selective pulse is replaced by a hard 180° pulse at its midpoint; numerical simulations show that the effect of using different shapes of selective pulse is to scale down this limiting gradient shift by a constant factor that depends on the pulse shape used. The practical consequence is that under the experimental conditions appropriate for small molecules, the pure shift iDOSY method should allow good diffusion coefficient measurements to be made if appropriate allowance is made for the change in effective diffusion-encoding gradient. Parallel sets of numerical simulations and experiments are presented, and a practical application of a Zangger-Sterk pure shift iDOSY experiment to a simple test mixture is illustrated.Files provided:Data====Figs. 2 to 4 and S1 to S4Figure 6a Oneshot-45Figure 6b ZS-iDOSYCode====Mathematica codeMGC analysis and figures.nbProcess simulated / experimental data for figs. 2 to 5 and S1 to S4Matlab codegradient_shift_analysis.mgradient_shift_test.mSimulate data for figs. 2 to 4 and S1 to S4Sequence codegmmgcFig. 1c, for figs. 2 to 4 and S1 to S4kp_ifZS-iDOSY_03Fig. 1a, for fig. 6b ZS-iDOSYkp_oneshot45_02Fig. 1d, for fig. 6a Oneshot-45N.B. The definition of d20 has been corrected to match the diffusion delay in these sequences, correcting errors in the sequences used for acquisition of, and found in, the data directories “Figs. 2 to 4 and S1 to S4” and “Figure 6b ZS-iDOSY”VnmrJ macrosGMgshiftCalculate and apply gradient value shiftGMPKBrukerNUGCalculate and apply amplitude and diffusion coefficient correction for gradient non-uniformityGMprocPK22Produce Fig. 6aGMprocPK1002Produce Fig. 6
Domestic aviation : a new direction for the 1990s : statement by the Minister for Transport and ..
No full texttag=1 data=Domestic aviation : a new direction for the 1990s : statement by the Minister for Transport and ...
tag=2 data=Evans, Gareth
tag=3 data=Australia.Dept of Transport and Communications
tag=6 data=^d7 ^mOct ^y1987
tag=8 data=AVIATION-AIRLINES
tag=9 data=AIRLINES AGREEMENT ACT 1981
tag=15 data=JOU
tag=32 data=MORRIS, PETE
Edge-transitive embeddings of complete graphs
No full textBuilding on earlier work of Biggs, James, Wilson and the author and on the Graver-Watkins description of the 14 classes of edge-transitive maps, we complete the classification of the edge-transitive embeddings of complete graphs, including those with non-empty boundary.</p
Serum steroid profiling for Congenital Adrenal Hyperplasia using liquid chromatography–tandem mass spectrometry
No full textBackground: Diagnosis of Congenital Adrenal Hyperplasia (CAH) is based on the quantification of 17-hydroxyprogesterone (17-OHP), usually by immunoassay. During the neonatal period the specificity of screening for CAH by blood spot 17-OHP immunoassay is low. High false-positive rates result in a relatively high demand for a second-tier serum confirmation test. A robust, specific and selective method for
measurement of cortisol, 21-deoxycortisol, 11-deoxycortisol, 4-androstene-3,17-dione (A4) and 17-OHP in serum has been developed. The method involves a simple extraction procedure and a fast analysis using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC/MS/MS).
Methods: The steroids were extracted from 50 μl of serum using methyl-tert-butyl-ether. Analysis was performed on a UPLC tandem quadrupole mass spectrometer system in positive mode electrospray ionization and multiple reaction monitoring acquisition.
Results: The assay was linear over each analyte concentration range with all correlation coefficients (r2)N 0.996. Inter- and intra-day CVs were ≤10% across the analytical range. In addition simultaneous measurement of the full range of steroids on the pathway to cortisol allows confirmation of the affected steroidogenic enzyme.
Conclusions: A second-tier test for the confirmation of CAH has been developed. The method allows for detection and quantification of 5 steroids related to CAH over the range of the clinical assay with good linearity, sensitivity and precision
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