130,780 research outputs found
ROC<sub>MoRF</sub> curves of multiple prediction tools.
No full text<p>Vertical axis is the true positive rate (TPR) and horizontal axis is the false positive rate (FPR). AUC<sub>MoRF</sub> values are in parentheses next to each label. (A) ROC<sub>MoRF</sub> curves of seven IDP predictors: DISOPRED (in black), ESpritz with the DisProt option (ESpritz_D in blue), ESpritz with the X-Ray option (ESpritz_X in yellow), ESpritz with the NMR option (ESpritz_N in green), IUPred with the long option (IUPred_l in brown), IUPred with the short option (IUPred_s in red), and SPINE-D (in light green). (B) The lower left corner of the ROC<sub>MoRF</sub> curves for the seven IDP predictors. (C) ROC<sub>MoRF</sub> curves of IPP (in blue), WOP (in gray), RWGRMP (in yellow) and its complement (Complement, in red). The Initial conservation propensity score, <i>ics</i>, (in green) was generated by joining IPP, Complement and WOP using Bayes rule. (D) ROC<sub>MoRF</sub> curves of ESpritz_D (in blue), <i>ics</i> (in green) and <i>mcs</i> (in red). <i>mcs</i> values are generated by processing <i>ics</i>.</p
Rektoratswechsel : an der Akademie für Sozial- und Handelswissenschaften zu Frankfurt a.M. /
No full text"Am 21. Oktober 1903."1. Rede des abtratenden Rektors Prof. Dr. H. Morf -- 2. Rede des antretenden Rektors Prof. Dr. K. Burchard.Mode of access: Internet
Image reconstruction algorithm for motion insensitive MR Fingerprinting (MRF): MORF
No full textPURPOSE: The purpose of this study is to increase the robustness of MR fingerprinting (MRF) toward subject motion. METHODS: A novel reconstruction algorithm, MOtion insensitive MRF (MORF), was developed, which uses an iterative reconstruction based retrospective motion correction approach. Each iteration loops through the following steps: pattern recognition, metric based identification of motion corrupted frames, registration based motion estimation, and motion compensated data consistency verification. The proposed algorithm was validated using in vivo 2D brain MRF data with retrospective in-plane motion introduced at different stages of the acquisition. The validation was performed using qualitative and quantitative comparisons between results from MORF, the iterative multi-scale (IMS) algorithm, and with the IMS results using data without motion for a ground truth comparison. Additionally, the MORF algorithm was evaluated in prospectively motion corrupted in vivo 2D brain MRF datasets. RESULTS: For datasets corrupted by in-plane motion both prospectively and retrospectively, MORF noticeably reduced motion artifacts compared with iterative multi-scale and closely resembled the results from data without motion, even when ∼54% of data was motion corrupted during different parts of the acquisition. CONCLUSIONS: MORF improves the insensitivity of MRF toward rigid-body motion occurring during any part of the MRF acquisition
Uterine leiomyomata with t(10;17) disrupt the histone acetyltransferase MORF
No full textBenign uterine leiomyomata are the most common tumors in women of reproductive age. One recurring chromosomal aberration in uterine leiomyomata is rearrangement of 10q22. Chromosome 10 breakpoints were mapped by fluorescence in situ hybridization to intervals ranging from 8.9 to 72.1 kb within the third intron of MORF (monocytic leukemia zinc finger protein-related factor or MYST4) in four uterine leiomyomata tested. Additional Southern hybridization experiments confirmed that the breakpoint lies within the third intron and narrowed the interval to 2.1 kb in one uterine leiomyomata. MORF is a member of the MYST family of histone acetyltransferase and previously has been found rearranged in some types of acute myeloid leukemia (AML). This is the first instance in which disruption of a histone acetyltransferase has been reported in another tumor type. The breakpoints in uterine leiomyomata would fall in the NH2-terminal portion of the protein between a conserved domain found in histones H1 and H5 and the PHD zinc fingers, the CH2CH zinc finger, or the CoA binding site, which is distinct from the breakpoints reported in AML. Mapping of the 17q21 breakpoint by fluorescence in situ hybridization within a specific region in three tumors revealed several positional candidates including GCN5L2, a gene with histone acetyltransferase activity similar to those fused to MORF in AML. Of note, two of three uterine leiomyomata were of the cellular subtype. Involvement of MORF in four uterine leiomyomata with chromosomal rearrangements involving 10q22 and 17q21 suggests a role for this histone acetyltransferase and altered chromatin regulation in uterine mesenchymal neoplasia.</p
Human Islet Cell MORF/cMORF Pretargeting in a Xenogeneic Murine Transplant Model
No full textNoninvasive measurement of human islet cell mass in pancreas or following islet transplantation by nuclear imaging has yet to be achieved. It has been shown using mouse tumor models that pretargeting imaging strategies are sensitive and can greatly increase target to nontarget signal ratios. The objective now is to demonstrate the specific pretargeting of human islet cells in mice. Our pretargeting strategy uses an anti-human islet cell antibody HPi1, conjugated to a phosphorodiamidate morpholino oligomer (MORF) that binds specifically to a (99m)Tc labeled complementary MORF (cMORF). Sensitivity and specificity of the pretargeting were first validated in culture using a human beta cell line (betalox5) and a negative control human cell line (HEK293). Pretargeting was then used to target and visualize these two cell lines and human islets transplanted subcutaneously in NOD-scid IL2rgamma(null) mice. In culture, (99m)Tc accumulation on the betalox5 cells pretargeted by MORF-HPi1 was 100-fold higher than on untreated betalox5 cells or following treatment with native HPi1 and much higher than on the MORF-HPi1 pretargeted control HEK293 cells. Small animal imaging readily localized the transplanted betalox5 cells and human islets, but not the HEK293 cells. Ex vivo counting demonstrated 3-fold higher (99m)Tc accumulation in the transplanted betalox5 cells and human islets than in the control HEK293 cells. The target accumulation was also shown to increase linearly with increased numbers of the implanted betalox5 cells. These results demonstrate specific binding of radioactivity and successful imaging of human betalox5 cells and human islets transplanted in mice. Thus MORF/cMORF pretargeting may be useful to measure noninvasively human islet cell mass within the pancreas or following islet transplantation
Histone H3K23-specific acetylation by MORF is coupled to H3K14 acylation
No full textAcetylation of histone H3K23 has emerged as an essential posttranslational modification associated with cancer and learning and memory impairment, yet our understanding of this epigenetic mark remains very limited. Here, we identified the native MORF complex as a histone H3K23-specific acetyltransferase and elucidated its mechanism of action.
The acetyltransferase function of the catalytic MORF subunit is positively regulated by the DPF domain of MORF (MORFDPF). The crystal structure of MORFDPF in complex with crotonylated H3K14 peptide provides mechanistic insight into selectivity of this epigenetic reader and its ability to recognize both histone and DNA. Mass spectrometry, biochemical and genomic analyses show co-existence of the H3K23ac and H3K14ac modifications in vitro and co-occupancy of the MORF subunits, H3K23ac, and H3K14ac at promoters of MORF target genes in vivo.
Together, our findings reveal a high correlation between two acetylation sites, H3K23 and H3K14, and suggest a model in which interaction of MORFDPF with acylated H3K14 promotes acetylation of H3K23 by the native MORF complex to activate gene transcription.journal articl
MeSH term explosion and author rank improve expert recommendations
No full textInformation overload is an often-cited phenomenon that reduces the productivity, efficiency and efficacy of scientists. One challenge for scientists is to find appropriate collaborators in their research. The literature describes various solutions to the problem of expertise location, but most current approaches do not appear to be very suitable for expert recommendations in biomedical research. In this study, we present the development and initial evaluation of a vector space model-based algorithm to calculate researcher similarity using four inputs: 1) MeSH terms of publications; 2) MeSH terms and author rank; 3) exploded MeSH terms; and 4) exploded MeSH terms and author rank. We developed and evaluated the algorithm using a data set of 17,525 authors and their 22,542 papers. On average, our algorithms correctly predicted 2.5 of the top 5/10 coauthors of individual scientists. Exploded MeSH and author rank outperformed all other algorithms in accuracy, followed closely by MeSH and author rank. Our results show that the accuracy of MeSH term-based matching can be enhanced with other metadata such as author rank
Human islet cell MORF/cMORF pretargeting in a xenogeneic murine transplant model
No full textNoninvasive measurement of human islet cell mass in pancreas or following islet transplantation by nuclear imaging has yet to be achieved. It has been shown using mouse tumor models that pretargeting imaging strategies are sensitive and can greatly increase target to nontarget signal ratios. The objective now is to demonstrate the specific pretargeting of human islet cells in mice. Our pretargeting strategy uses an anti-human islet cell antibody HPi1, conjugated to a phosphorodiamidate morpholino oligomer (MORF) that binds specifically to a (99m)Tc labeled complementary MORF (cMORF). Sensitivity and specificity of the pretargeting were first validated in culture using a human beta cell line (betalox5) and a negative control human cell line (HEK293). Pretargeting was then used to target and visualize these two cell lines and human islets transplanted subcutaneously in NOD-scid IL2rgamma(null) mice. In culture, (99m)Tc accumulation on the betalox5 cells pretargeted by MORF-HPi1 was 100-fold higher than on untreated betalox5 cells or following treatment with native HPi1 and much higher than on the MORF-HPi1 pretargeted control HEK293 cells. Small animal imaging readily localized the transplanted betalox5 cells and human islets, but not the HEK293 cells. Ex vivo counting demonstrated 3-fold higher (99m)Tc accumulation in the transplanted betalox5 cells and human islets than in the control HEK293 cells. The target accumulation was also shown to increase linearly with increased numbers of the implanted betalox5 cells. These results demonstrate specific binding of radioactivity and successful imaging of human betalox5 cells and human islets transplanted in mice. Thus MORF/cMORF pretargeting may be useful to measure noninvasively human islet cell mass within the pancreas or following islet transplantation
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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