1,721,006 research outputs found
DETECTION OF PESTIVIRUS IN FECAL SAMPLES FROM WILD RUMINANTS BY PCR
Ruminant pestiviruses affect wild and domestic
ruminants worldwide, causing reproductive disorders
and severe economic losses. Usually serological,
virological or PCR tests are carried out on
serum or blood collected from captured live animals
or from organs of dead animals. The aim of
this work was to evaluate if fecal samples are
suitable for detecting ruminant pestiviruses in
wild ruminants.
Fecal samples from red deer (Cervus elaphus,
n=16) and Appenninic chamois (Rupicapra pyrenaica
ornata, n=12) were collected from the environment.
Samples were analyzed in a few days
and aliquots were stored at 4oC for 3 months
and re-tested. Pooled fecal samples were diluted
with PBS, were centrifuged, and the pellet was
used for RNA extraction by silica membrane tech-nology. Real Time PCR was carried out to detect
Pestivirus spp. Positive samples were used for
PCR and sequencing of a 5’UTR sequence.
All four pool samples from red deer and 3 out of
4 pool of chamois resulted positive and sequences
showed the highest homology with
BVDV-1. Positive samples were found also in
samples refrigerated for 3 months.
To our knowledge, this is the first report of amplification
and sequencing of pestiviruses from
feces of wild ruminants
Maestri di Umanesimo nell'Italia nord-orientale del Quattrocento
Il rapido estendersi del dominio veneziano determinò, agli inizi del Quattrocento, la rottura della continuità delle istituzioni politiche su cui si era appoggiata la cultura umanistica locale. Sorta precocemente in area veneta, essa fu costretta a misurarsi con un nuovo interlocutore, quale la Repubblica di Venezia, molto diverso da quelli che ne avevano favorito l'acerba nascita e poi, nel Trecento, ne avevano sostenuto lo straordinario sviluppo.
Partendo dalla Padova del XIII secolo, definita come “la prima capitale dell'Umanesimo italiano”, il contributo ricostruisce alcune figure di educatori umanisti - in particolare Pier Paolo Vergerio (1370-1444) e Francesco Barbaro (1390-1454) - e la grande influenza esercitata dall'ambiente veneto per il fiorire delle attività e delle esperienze pedagogiche del Quattrocento, come pure la sua stretta relazione con il mondo lombardo
Guarino Veronese. Cenni storiografici e di ricerca
Analisi degli studi condotti su Guarino Veronese dall'Ottocento ai nostri giorni con proposte per future ricerche sempre su Guarino Veronese e l'ambiente a lui collegato
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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