1,721,005 research outputs found

    Construction and analysis of the miscanthus genespace

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    Miscanthus and energy cane are closely related perennial grasses and candidate bioenergy crops. Both genomes create a challenge for variant discovery and genotyping because of their abundant repeats and the presence of a genome scale duplication with little subsequent divergence. In addition, the Miscanthus genome has high heterozygosity due to its self-incompatible breeding system. This complexity necessitates the discovery of sequence variants that distinguish paralogs from alleles, which are most frequent in the low-copy non-coding fraction of the genespace. To enrich for such sequences from Miscanthus genomes, we developed a sequence capture array using a solution-based hybridization method. The probe set was designed to capture exons and flanking intronic sequences predicted from alignment of Miscanthus transcriptome, fosmid, and genomic reads to the largely syntenic Sorghum genome. To facilitate haplotype discovery, we selected for large insert sizes that were subsequently sequenced at high depth as 100x75 bp paired-end reads. Chapter 1 reports the sequence capture of a doubled haploid Miscanthus sinensis plant and its parent, and chapter 2 reports the sequence capture of energy cane and two other Miscanthus species. This sequence capture design was able to distinguish variation at high depth and it was found that amplification is imperative to achieve this depth. Further analysis of the reads verified that Miscanthus has undergone recent whole genome duplication. Additionally, sequence capture serves as a useful tool for comparative genomics and provides strong evidence that Miscanthus can serve as a satisfactory reference to the closely related, yet highly complex genomes of Saccharum spp.Item withdrawn by Laura Spradlin ([email protected]) on 2013-12-05T22:23:47Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Kirkpatrick_Jessica.docx: 10188025 bytes, checksum: 53127ec096137ab97226c031706ab4d0 (MD5) Kirkpatrick_Jessica.pdf: 5703079 bytes, checksum: 468be0df75dc55bc72d38c7b9b2feb16 (MD5)Made available in DSpace on 2014-01-16T18:26:31Z (GMT). No. of bitstreams: 3 Jessica_Kirkpatrick.pdf: 5703079 bytes, checksum: 468be0df75dc55bc72d38c7b9b2feb16 (MD5) Kirkpatrick_Jessica.docx: 10188025 bytes, checksum: 53127ec096137ab97226c031706ab4d0 (MD5) license.txt: 4069 bytes, checksum: 8a4d0469e3519898482cfd50c2b0714f (MD5)Restriction data tranferred 2014-07-01T11:36:50-05:00 Original Data Group with Access Administrator Release Date: 2016-01-16 12:27:27 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemItem marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins ([email protected]) on 2014-01-16T18:27:38Z Item is restricted until 2016-01-16T18:27:27ZLimited Restriction Lifted for Item 46944 on 2016-01-16T11:00:39Z

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Effect of nitrogen form and genetic variations of the Asparagine-cycling pathway on maize ear growth

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    The positive growth response of maize ears to nitrate supplementation declines during late vegetative development, whereas ammonia addition continues to promote ear growth through the reproductive phase. The underlying genetic and physiological mechanisms for this phenomenon are currently unknown. Previous studies with DuPont Pioneer found that in the maize line Gaspé Flint, ear growth no longer responded to additional nitrate 20 days after emergence, but a subsequent addition of ammonia significantly increased ear dry weight as well as the transport amino acids asparagine and glutamine. This nitrogen-induction experiment was duplicated, and root, shoot, and ear tissues were profiled via RNA-Seq to characterize responses to ammonia or nitrate at four or eight hours after nitrogen treatment. Results suggest an upregulation of amino acid assimilation and metabolism genes in ammonia-treated plants. Illinois High Protein (IHP) differs from other genotypes in exhibiting an increased rate of nitrogen uptake, vigorous ear growth under low N conditions, very high levels of asparagine, but a reduced response to ammonia. These observations suggest that the mechanism for ammonia-induced ear growth in some genotypes may already be active in IHP even without ammonia supplementation. Compared to many other maize genotypes, IHP has functional variants of the two asparagine-cycling genes, Asparagine synthetase (AS) and Asparaginase (ASNase). We examined the effect of these two genes on ear growth using a range of populations that contain variants of these genes. Greater AS expression was associated with improved ear growth, whereas very low ASNase activity was associated with decreased developing ear size.Item withdrawn by Mark Zulauf ([email protected]) on 2014-04-30T20:54:09Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 6 Cody Postin Thesis.docx: 2686001 bytes, checksum: f440ab84d17d4dbe55d333836f91b741 (MD5) Supplemental File 4 (Grain NIR).xlsx: 75780 bytes, checksum: dcbaecfa074c05564f4098cd4a0f2fdb (MD5) Supplemental File 3 (Grain & Cob).xlsx: 84019 bytes, checksum: 5b10bae10d2a036b70b59257f8f8b15a (MD5) Supplemental File 2 (Earshoot Amino Acids).xlsx: 104199 bytes, checksum: a989b962b09f5dcff4e220011b7d2c8d (MD5) Supplemental File 1 (Earshoot Size).xlsx: 100938 bytes, checksum: 9f261a57d56ff80438777f295d6db937 (MD5) Postin_Cody.pdf: 1879151 bytes, checksum: ad1ec590fdebcf29aaf4b75c688ea2ed (MD5)Made available in DSpace on 2014-05-30T17:17:22Z (GMT). No. of bitstreams: 7 Cody_Postin.pdf: 1879151 bytes, checksum: ad1ec590fdebcf29aaf4b75c688ea2ed (MD5) Supplemental File 4 (Grain NIR).xlsx: 75780 bytes, checksum: dcbaecfa074c05564f4098cd4a0f2fdb (MD5) Supplemental File 3 (Grain & Cob).xlsx: 84019 bytes, checksum: 5b10bae10d2a036b70b59257f8f8b15a (MD5) Supplemental File 2 (Earshoot Amino Acids).xlsx: 104199 bytes, checksum: a989b962b09f5dcff4e220011b7d2c8d (MD5) Supplemental File 1 (Earshoot Size).xlsx: 100938 bytes, checksum: 9f261a57d56ff80438777f295d6db937 (MD5) Cody Postin Thesis.docx: 2686001 bytes, checksum: f440ab84d17d4dbe55d333836f91b741 (MD5) license.txt: 4060 bytes, checksum: 72bb1c243887ec335878c2538598254f (MD5)Item marked as restricted to the 'Administrator' Group (id=1) by Seth Robbins ([email protected]) on 2014-05-30T17:21:26Z Item is restricted until 2016-05-30T17:21:23ZRestriction data tranferred 2014-07-01T11:39:37-05:00 Original Data Group with Access Administrator Release Date: 2016-05-30 12:21:23 UTC Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemLimited Restriction Lifted for Item 49832 on 2016-09-22T20:59:22Z

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Unlocking the genetic diversity of the undomesticated rice relative Oryza longistaminata

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    Rice (Oryza sativa) is a crucial part of the global food supply. Meeting projected increases in demand, which are rising fastest in sub-Saharan Africa, while using resources efficiently and adapting to a changing climate will require new sources of variation for trait improvement. The genetically diverse undomesticated African rice, Oryza longistaminata, which is native to a broad range of habitats throughout sub-Saharan Africa, is a valuable resource improving O. sativa with genes conferring biotic and abiotic stress tolerances, increased yield, super-ratooning ability, and floral traits conducive to hybrid rice production. The major obstacle to utilization of O. longistaminata in breeding programs is a breeding barrier which complicates interspecific hybridization with O. sativa, as well as the expense and difficulty of identifying which O. longistaminata accessions carrying useful genes. Breeding programs also rely on thorough ex-situ conservation of O. longistaminata, which enables long-term access to germplasm for breeders and other stakeholders. In this work, three genetic subpopulations were identified in O. longistaminata using individuals representing most of the species’ range genotyped with densely-spaced, genome-wide molecular markers. Spontaneous interspecific hybrids of O. longistaminata and O. sativa were discovered in germplasm from the International Rice Research Institute’s genebank, which can be used to accelerate introgression from O. longistaminata to O. sativa. A species distribution model of O. longistaminata was generated to identify sampling gaps in ex-situ collections, and environmental data was used to predict likely locations of O. longistaminata tolerant to abiotic stresses.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-08-01The student, Marlee Labroo, accepted the attached license on 2017-07-20 at 15:00.The student, Marlee Labroo, submitted this Thesis for approval on 2017-07-20 at 15:04.This Thesis was approved for publication on 2017-07-20 at 17:55.DSpace SAF Submission Ingestion Package generated from Vireo submission #11557 on 2017-09-29 at 11:20:02Made available in DSpace on 2017-09-29T17:52:31Z (GMT). No. of bitstreams: 2 LABROO-THESIS-2017.pdf: 1318508 bytes, checksum: eb2362b5ea3c775758c33750896fdb75 (MD5) LICENSE.txt: 4210 bytes, checksum: 0110d139cef596ee8f586d4a09628420 (MD5) Previous issue date: 2017-07-20Embargo set by: Colleen Fallaw for item 103516 Lift date: 2019-09-29T17:52:45Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemLimited Restriction Lifted for Item 103516 on 2019-09-30T09:15:23Z

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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