1,444 research outputs found
Universal home network middleware guaranteeing seamless interoperability among the heterogeneous home network middleware
No full textUsers will be able to access ubiquitously present appliances anywhere and anytime through home network. For this, we need middleware that provides a high-level abstraction, self-configuration, and guarantees the interoperability among middleware. However, most home network middleware does not ensure interoperability with different middleware. This paper presents the UHNM architecture, which guarantees seamless interoperability among the heterogeneous home network middleware for future home. The UHNM provides the high-level abstraction and zero-configuration, and makes new services without great effort.(1)
Design of a universal middleware bridge for device interoperability in heterogeneous home network middleware
No full textThis paper proposes the design of the software Universal Middleware Bridge (UMB) that can be used to solve seamless interoperability problems caused by the heterogeneity of several kinds of home network middleware. We verified that the proposed UMB dynamically maps physical devise in all different middleware domains into virtually abstracted devices in the UMB domain and enables all home devices overlaid on heterogeneous networks to be seen as virtually the same physical devices in the same middleware domain, as well as to detect and control each other. As a result, it is concluded that the proposed architecture provides commercial feasibility and cost benefic for the system implementations(1).The authors wish to thank Professors Young-Hee Lee for his
contributions to design of UMB
Native PAGE experiments on SL1-wt RNA kissing dimer (KD) in the presence of sub-stoichiometric amounts of NCp7, run in the TBE buffer
No full text<p><b>Copyright information:</b></p><p>Taken from "Nucleocapsid protein-mediated maturation of dimer initiation complex of full-length SL1 stemloop of HIV-1: sequence effects and mechanism of RNA refolding"</p><p></p><p>Nucleic Acids Research 2007;35(6):2026-2034.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1874624.</p><p>© 2007 The Author(s)</p> KD of SL1-wt was incubated with NCp7 at ambient temperature for 18 h in the RNA strand-to-protein ratio of 2:0, 2:2, 2:1.6, 2:0.8, 2:0.4 and 2:0.2, as indicated. The upper band in each lane (‘LD’) corresponds to mature linear dimer, while the lower monomer band (‘M’) emanates from the residual KD that dissociates during PAGE in the TBE buffer. Increasing intensity of the monomer band and simultaneous decrease in the dimer band across the lanes correlates with the amount of NCp7 present in each complex
Therapeutic studies in hepatic encephalopathy
No full textThis article has arisen from presentations made at the 4th International Hannover Conference on Hepatic Encephalopathy held in Dresden, 2006. Each author as listed describes their presentation given as part of a section entitled "Therapeutic Studies in Hepatic Encephalopathy." The first section deals with the justification for placebo-controlled trials in hepatic encephalopathy. The other two sections discuss, in detail, outcome parameters for therapeutic studies in the clinical and research setting, respectivel
Concurrent linearizable nearest neighbour search in lockfree-kd-Tree
No full textThe Nearest neighbour search (NNS) is a fundamental problem in many application domains dealing with multidimensional data. In a concurrent setting, where dynamic modi-fications are allowed, a linearizable implementation of NNS is highly desirable. This paper introduces the LockFree-kD-Tree (LFkD-Tree): A lock-free concurrent kD-Tree, which implements an abstract data type (ADT) that provides the operations Add, Remove, Contains, and NNS. Our implementation is linearizable. The operations in the LFkD-Tree use single-word read and compare-And-swap (CAS) atomic primitives, which are readily supported on available multi-core processors. We experimentally evaluate the LFkD-Tree using several benchmarks comprising real-world and synthetic datasets. The experiments show that the presented design is scalable and achieves signi cant speed-up compared to the implementations of an existing sequential kD-Tree and a recently proposed multidimensional indexing structure, PH-Tree.\ua0\ua9 2018 Copyright held by the owner/author(s)
Dispersive to nondispersive transition in the plane wake and channel flows
No full textBy varying the wavenumber over a large and finely discretized interval of values, we analyse the phase and group velocity of linear three-dimensional travelling waves both in the plane wake and channel flows to get the transition between dispersive and non-dispersive behaviour. The dispersion relation is computed from the Orr-Sommerfeld and Squire eigenvalue problem by observing the least stable mode, see figure 2, panels (a,b) and the comparison with [1, 2, 4–11, 15, 16]. The group velocity vg is also shown. The Reynolds number varies in the 20-100, 1000-8000 ranges for the wake and the channel flow, respectively, while we consider wavenumbers in the range 0.1-10. The wake basic flow consists of the first two orders of the Navier-Stokes matched asymptotic expansion described in [3, 13, 14]. At low wavenumbers we observe a dispersive behaviour where the phase speed and the group velocity substantially differ. The relevant perturbed solution is amenable to the typical solution belonging to the left branch of the eigenvalue spectrum, see the two examples shown in figure 1 (channel flow: Re = 6000; k = 1; wake Re = 100; k = 0:7). By rising the wave number value, we observe a sharp transition from the dispersive to the nondispersive regime. This transition is located at a critical wave number kd which is a function of the Reynolds number Re, the wave angle _, and the wake downstream observation point x0. Precisely, kd increases with Re and decreases with _ for the wake flow, while these trends are reversed for the channel flow, see tables 1,2. Beyond the wavenumber threshold, the observed least-stable mode belongs to the right branch of the spectrum. The asymptotic solutions in the dispersive region are wall modes for the channel flow , and in-wake modes for the wake flow. This means that, for both the flows, the dispersive behaviour is related to perturbations with high momentum variations (high vorticity) in correspondence to the base flow high-shear region. On the contrary, if k > kd the solutions are central modes for the channel case, and out-of-wake modes for the wake flow. In these cases, the disturbance has high variations outside the base flow high-shear region. To understand the physical mechanism of the dispersive-nondispersive transition we focused on time variation of the wave kinetic energy associated to the convective transport. Figure 2 (c,d) shows the convective term as a function of the wavenumber for the two least stable modes. We observe that the dispersive-nondisperive transition allows waves k > kd to keep the lowest possible temporal variation of kinetic energy, i.e. the lowest decay. This remains true also when all the other more stable modes are considered. In practice nondispersive waves maintain their convective energy with k
Towards a second-generation robotic telescope mount for the air-LUSI instrument
No full textEarth observation satellites, such as those responsible for monitoring the effects of climate change, require rigorous calibration protocols to account for on-orbit sensor degradation. An increasingly dependable method to address this issue uses the Moon as a reference light source for in-situ calibration. The airborne lunar spectral irradiance (air-LUSI) mission aims to improve the utility of the Moon as an on-orbit calibration target for remote sensing instruments, by tying the currently accepted lunar model to the SI and establishing lunar irradiance on an absolute scale. To this end, air-LUSI collects SI-traceable measurements of lunar irradiance at visible to near-infrared wavelengths with unprecedented accuracy. A non-imaging telescope is flown at an altitude of 21 km, aboard NASA's high-altitude ER-2 aircraft, which places the instrument above 95% of the Earth's atmosphere for clean, minimally obstructed lunar spectra. To fix the optical axis on the Moon during flight, an autonomous control system is required to compensate for aircraft motion and track the Moon across its celestial transit. In this paper, we present an overview of the robotic subsystem used to track the Moon on more than ten high-altitude flights, and the valuable lessons learned from those campaigns. From this insight, a preliminary design for a second-generation robotic telescope mount is presented. Referred to as the HAAMR, it will supplant the current robotics system on future air-LUSI Operational Flight Campaigns, with the nearest field deployment slated for January 2024. We show how this new system is poised to offer a more reliable, accurate, and responsive platform for the air-LUSI instrument to continue collecting data that will ultimately help to improve our understanding of the Earth's climate
Characterization of [3H]CGP 12177 binding to beta-adrenergic receptors in intact eel hepatocytes
No full textThe aim of this study was to characterize [3H]CGP 12177 (CGP) binding to β-adrenergic receptors in isolated hepatocytes of the European eel (Anguilla anguilla), in which the involvement of cAMP in epinephrine-induced glucose release has been previously observed. Specific binding of CGP was saturable, reversible, and linear as a function of cell number. Analysis of binding data suggested a single class of binding sites, with a Kd of 1.31 nM and a number of approximately 7000 β-adrenergic receptors per cell. The potency order of specific inhibition of [3H]CGP binding was CGP > propranolol ≥ alprenolol ≫ butoxamine ≥ atenolol, while phentolamine and prazosin failed to significantly displace the tracer at concentrations up to 100 μM. The binding kinetics of CGP were closely related to its biological effect. In fact, the drug dose-dependently counteracted the enhancement of intracellular cAMP levels induced by epinephrine in isolated hepatocytes with a Kd of 1.06 nM. Moreover, it antagonized the hormone-induced stimulation of adenylyl cyclase activity in hepatic membranes as well as of glucose release from cells. These data clearly show that β-adrenergic receptors are coupled to the adenylyl cyclase/cAMP transduction pathway in eel liver. © 2001 Academic Press
Expanding Indications for a Ketogenic Diet as an Adjuvant Therapy in Adult Refractory Status Epilepticus: an Exploratory Study Using Moderation Analysis
No full textRefractory status epilepticus (RSE) requires multimodal treatment approaches to achieve rapid seizure cessation and neuroprotection. A ketogenic diet (KD) has demonstrated efficacy as a nutritional therapeutic option for adult RSE. However, the group of adult RSE patients who would benefit from adopting a KD needs to be determined to appropriately select the patients indicated for a KD. Therefore, we conducted a nonrandomized retrospective cohort study to explore the therapeutic efficacy of a KD by investigating the moderation effect of a KD on the association between the clinical characteristics of RSE patients and their functional outcomes. This study investigated 140 RSE patients, including 32 patients treated with a KD; among these patients, 28 (81%) achieved seizure cessation. We found that KD moderated the reduction in the modified Rankin scale (mRS) score at discharge among patients who were older, had higher seizure severity scores, were under continuous intravenous anesthetic therapy (CIVAD), and had super-RSE. Age and seizure severity scores, but not CIVAD or super-RSE, were associated with a KD-moderated change in mRS score at 3 months. Thus, we consider that our study provides evidence of a neuroprotective effect of KD in the most severe RSE patients with very few remaining therapeutic options, but future randomized controlled trials in these subgroups of KD patients are necessary
Differentiating <i>Ell3</i>-KD cells undergo apoptosis, which is associated with enhanced p53 expression and activated caspase pathway.
No full text<p>(A) Cell cycle distribution of control and <i>Ell3</i>-KD cells stained with propidium iodide (PI). Cells in the ESC state or differentiated for 3 days (RA-D3) by removing LIF and adding retinoic acid (RA) were analyzed by flow cytometry. (B) Apoptosis of control and <i>Ell3-</i>KD cells was quantitatively analyzed either in the ESC or differentiated state by determining the number of Annexin V-positive cells. Cells were spontaneously differentiated for 3 days (RA-D3). (C) The amounts of Lamin B in control or <i>Ell3</i>-KD cells were determined either in the ESC state or in spontaneously differentiated cells for 3 days (RA-D3) by immunoblot analysis. β-actin was used as a loading control. Control (V) or <i>Ell3</i>-expressing vectors (<i>Ell3</i>) were transfected into <i>Ell3</i>-KD cells in the ESC state, and transfected cells were spontaneously differentiated for 3 days. Cells were examined under the microscope (D) and apoptosis was quantitatively analyzed by determining the number of Annexin V-positive cells (E). (F) The amounts of Lamin B, procaspase-3, and procaspase-9 in <i>Ell3</i>-KD cells transfected with control or <i>Ell3</i>-expressing plasmids were determined either in the ESC state or in spontaneously differentiated cells after 3 days by immunoblot analysis. β-actin was used as a loading control. (G) <i>Ell3</i>-KD cells were transfected with control (V) or <i>Ell3</i>-expressing plasmids (Ell3). Transfected cells were spontaneously differentiated for 3 days, and <i>p53</i> levels were examined by immunoblot analysis. β-actin was used as a loading control. (H) <i>p53</i> in control or <i>Ell3</i>-OE cells was determined after 3 days of spontaneous differentiation by immunoblot analysis (left panel). <i>Ell3</i>-OE cells were transfected with nonspecific siRNA (siNS) or <i>Ell3</i>-targeting siRNA (si<i>Ell3</i>). Transfected cells were spontaneously differentiated for 3 days, and <i>p53</i> was examined by immunoblot analysis (right panel). β-actin was used as a loading control. All experiments were performed at least in triplicate and all values represent the mean ± s.d. from at least triplicate experiments. * Indicates significant (P<0.05) and ** highly significant (p<0.01) results (Student's t-test).</p
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