63,004 research outputs found
Crystallization and preliminary crystallographic studies of Sfp: a phosphopantetheinyl transferase of modular peptide synthetases
No full textThe Bacillus subtilis Sfp protein is required for the non-ribosomal biosynthesis of the lipoheptapeptide antibiotic surfactin. It converts seven peptidyl carrier protein (PCP) domains of the surfactin synthetase SfrA-(A-C) to their active hole-forms by 4'-phosphopantetheinylation. The B. subtilis sfp gene was overexpressed in Escherichia coli and its gene product was purified to homogeneity and crystallized. Well diffracting single crystals were obtained from Sfp as well as from a selenomethionyl derivative, using sodium formate as a precipitant. The crystals belong to the tetragonal space group P4(1)2(1)2/P4(3)2(1)2, with unit-cell parameters a = b = 65.3, c = 150.5 Angstrom. They diffract beyond 2.8 Angstrom and contain one molecule in the asymmetric unit
Crystal structure of the surfactin synthetase-activating enzyme Sfp: a prototype of the 4 '-phosphopantetheinyl transferase superfamily
No full textThe Bacillus subtilis Sfp protein activates the peptidyl carrier protein (PCP) domains of surfactin synthetase by transferring the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue conserved in all PCPs. Its wide PCP substrate spectrum renders Sfp a biotechnologically valuable enzyme for use in combinatorial non-ribosomal peptide synthesis. The structure of the SfpCoA complex determined at 1.8 Angstrom resolution reveals a novel alpha/beta-fold exhibiting an unexpected intramolecular 2-fold pseudosymmetry, This suggests a similar fold and dimerization mode for the homodimeric phosphopantetheinyl transferases such as acyl carrier protein synthase, The active site of Sfp accommodates a magnesium ion, which is complexed by the CoA pyrophosphate, the side chains of three acidic amino acids and one water molecule. CoA is bound in a fashion that differs in many aspects from all known CoA-protein complex structures. The structure reveals regions likely to be involved in the interaction with the PCP substrate
Thermal expansion anomalies of R(Fe, M)(12) (R=Y, Nd; M=Mo and Si)
No full textStructural and thermal-expansion anomaly studies on R(Fe,M)(12) (R=Nd and and Y, M=Mo and Si) compounds were performed by x-ray diffraction. Mo atoms occupy the 8i site. While Si atoms occupy the 8f and 8j sites but not the 8i site. Thermal-expansion anomaly shows only in ab plane in the Mo compounds, while becomes very weak and along with only the c axis in the Si compounds. The anomaly was attributed to the contribution of the interactions of short Fe-Fe distances similar to the previous explanation on other R-Fe intermetallics and that of other strongly positive interactions such as 8j-8j. (c) 2005 American Institute of Physics.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000230168300025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Physics, AppliedSCI(E)EICPCI-S(ISTP)
Letter from Thomas R. Bodine, American Friends Service Committee Seattle office, to Mary M. Kimber, May 25, 1942
No full textLetter from Thomas R. Bodine to Mary M. Kimber, asking Kimber to visit individuals from the Puget Sound area incarcerated at Pinedale Assembly Center: Rev. Daisuke Kitigawa, Waichi Oyanagi, Chisako Higuchi, Mutsuo Hasiguchi and Mrs. Matsuoka, Makato Kobukata, the Hirabayashi family, and Violet Yokoyama. A note in pencil at the top of the page: "Burcham." A response letter from Grace and Calvin Coke to Thomas R. Bodine is found in item: chs_ms840_0306.Personal correspondence, organizational records, government documents, publications, and other papers created or collected by Joseph R. Goodman documenting the forced removal and incarceration of Japanese Americans during World War II, as well as organized resistance to incarceration. Included in the collection are records of the Japanese Young Men's Christian Association and the Japanese American Citizens' League in San Francisco, including papers of the Japanese YMCA's executive secretary Lincoln Kanai; Sakai family papers; Goodman's correspondence to and from Japanese American incarcerees, organizations opposing forced removal and incarceration of Japanese Americans, the War Relocation Authority, and others; publications, photographs, and ephemera from the Topaz Relocation Center, where Goodman taught high school; War Relocation Authority records and publications; and newspaper clippings, pamphlets, and reports about forced removal and incarceration created by various government, religious, and civic organizations, in California and nationwide
A 2 h periodic variation in the low-mass X-ray binary Ser X-1
No full textSpectroscopy of the low-mass X-ray binary Ser X-1 using the Gran Telescopio Canarias have revealed a ?2 h periodic variability that is present in the three strongest emission lines. We tentatively interpret this variability as due to orbital motion, making it the first indication of the orbital period of Ser X-1. Together with the fact that the emission lines are remarkably narrow, but still resolved, we show that a main-sequence K dwarf together with a canonical 1.4 M? neutron star gives a good description of the system. In this scenario, the most likely place for the emission lines to arise is the accretion disc, instead of a localized region in the binary (such as the irradiated surface or the stream-impact point), and their narrowness is due instead to the low inclination (?10°) of Ser X-1
"Closing the R&D Gap, Evaluating the Sources of R&D Spending"
No full textBoth spending and tax policies have been implemented in the United States with the goal of stimulating private sector research and development (R&D). Karier questions whether current R&D policy, especially the research and experimentation tax credit, can contribute to closing the gap between nondefense expenditures on R&D in the United States and such expenditures in other countries, such as Japan and Germany. He also explores possible changes to our current R&D policy to make it more effective.
Liftings for noncomplete probability spaces
No full textThe current state of knowledge concerning liftings for noncomplete probability spaces is discussed. This is a somewhat expanded version of the author's talk given at the 1991 Summer Conference on General Topology and Applications in Honor of Mary Ellen Rudin and Her Work.PT: S; CR: BURKE MR, IN PRESS P AM MATH S BURKE MR, 1991, ISRAEL J MATH, V73, P33 BURKE MR, 1992, ISRAEL J MATH, V79, P289 CARLSON T, THEOREM LIFTING CHRISTENSEN JPR, 1974, TOPOLOGY BOREL STRUC FREMLIN DH, 1989, HDB BOOLEAN ALGEBRAS, P877 INOESCUTULCEA A, 1966, 5TH P BERK S MATH ST, V2 IONESCUTULCEA A, 1967, CONTRIBUTIONS PROB 1, P63 IONESCUTULCEA A, 1969, TOPICS THEORY LIFTIN JECH TJ, 1978, SET THEORY JOHNSON RA, 1980, P AM MATH SOC, V80, P234 JUST W, IN PRESS T AM MATH S KUPKA J, 1983, INDIANA U MATH J, V32, P717 LOSERT V, 1983, LNM, V1080, P95 MAHARAM D, 1958, P AM MATH SOC, V9, P987 SHELAH S, 1983, ISRAEL J MATH, V45, P90 TALAGRAND M, 1982, P AM MATH SOC, V84, P379 VONNEUMANN J, 1931, CRELLES J MATH, V165, P109; NR: 18; TC: 0; J9: ANN N Y ACAD SCI; PG: 4; GA: BZ86BSource type: Electronic(1
Preparation of (R)-2-chloro-1-(m-chlorophenyl)ethanol by Lipozyme TL IM-catalyzed second resolution
No full text(R)-2-Chloro-1-(m-chlorophenyl)ethanol, a precursor of (R)-3-chlorostyrene oxide which is the key chiral intermediate for the preparation of several beta 3-adrenergic receptor agonists was prepared in 40% yield and 99% ee by the Lipozyme TL IM-catalyzed second resolution of the corresponding racemate in the presence of vinyl acetate. (C) 2012 Shi Wen Xia. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved
De Maiestate / Praeside M. Jacobo Thomasio, Moralis Philosoph. P. P., publice disputabit Johannes Dunte, R. L. Author & Respon: ad diem 9. Septembr. H L. Q. C.
No full textDE MAIESTATE / PRAESIDE M. JACOBO THOMASIO, MORALIS PHILOSOPH. P. P., PUBLICE DISPUTABIT JOHANNES DUNTE, R. L. AUTHOR & RESPON: AD DIEM 9. SEPTEMBR. H L. Q. C.
De Maiestate / Praeside M. Jacobo Thomasio, Moralis Philosoph. P. P., publice disputabit Johannes Dunte, R. L. Author & Respon: ad diem 9. Septembr. H L. Q. C. (1)
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Corrigendum to “Presence and function of kisspeptin/KISS1R system in swine ovarian follicles” (Theriogenology (2018) 115 (1–8), (S0093691X1830147X), (10.1016/j.theriogenology.2018.04.006))
No full textThe authors regret the following changes to the author group G. Basinia, F. Grassellia, S. Bussolatia, R. Ciccimarraa, M. Maranesib, A. Bufalarib, C. Dall'Agliob, F. Parilloc,#, M. Zeranib,c,*. a Dipartimento di Scienze Mediche Veterinarie, Università di Parma, 43126 Parma, Italy. b Dipartimento di Medicina Veterinaria, Università di Perugia, 06126 Perugia Italy. c Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62024 Matelica Italy. # Deceased. * Corresponding author: tel.: +39 0755857642; fax +39 0755857654. E-mail address: [email protected] (M. Zerani). And to the acknowledgements and figures
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