1,720,964 research outputs found
Function and evolution of Protein kinase A splice variant Cβ2 and its role as a biomarker in prostate cancer
No full textTranscriptome profiling of LH-producing gonadotropes in female medaka - with special emphasis on calcium-activated potassium channels
No full textThe brain-pituitary-gonad axis regulates puberty and reproduction in all vertebrates. The two gonadotropins, FSH and LH are released from the pituitary and initiates maturing and growth of the gonads. To understand more of the changes that take place in the gonadotropes during puberty, the transcriptome of LH-producing gonadotropes from adult and juvenile medaka were profiled with high-throughput sequencing. The pituitaries were sampled from genotyped females from a (lhb:GFP) transgenic line. LH-producing cells were sorted out by FACS. RNA was isolated from the sorted cells, amplified, converted to cDNA, fragmented and sequenced with Illumina.
The sequence output from Illumina is called reads, and the reads were aligned to the medaka genome with software called TopHat. Reads were counted with the software HTSeq, and differentially expressed genes were located with the software DESeq. Some of the expressed genes that are known to be involved in reproduction, like, LHβ-subunit, GnRH-R’s and dopamine receptors were focused on. The LHβ-subunit was highly expressed in both adult and juveniles. The analysis shows low expression of GnRH-R-3 in the adults. The dopamine receptors D1, D2 and D3 were all expressed. Plenty of the differential expression analysis between juvenile and adult sample remains. However, the material has potential to contribute to the understanding how the gonadotropes mature.
In addition, KCa channel genes were investigated further since these channels are believed to be involved in the excitability of endocrine cells and thus important for secretion control. KCa channels were obtained from eel and cod, in addition to medaka by blasting their respective genomes with known KCa channel sequences. In eel only the BK channel, the SK1 channel and the SK2 channel sequences were obtained, while all the channel sequences were obtained from medaka and cod. The obtained sequences were analysed by sequence alignments and phylogenetic analysis, in addition to the output from RNA-seq. All KCa channels were expressed in both juvenile and female medaka, except for one variant of the SK1 channel. This particular variant of SK1 also showed a different phylogenetic relationship, than the other SK1 channels, suggesting that the channel type is non-functional. Phylogenetic analyses were performed on all the KCa channels to find the phylogenetic relationship according to species and channel type. The phylogenetic analyses showed the expected relationship according to channel type and species
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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