4,433 research outputs found
Clustering and dynamics of cytochrome bd-I complexes in the Escherichia coli plasma membrane in vivo.
No full textThe cytochrome bd-I complex of Escherichia coli is a respiratory terminal oxidase and an integral component of the cytoplasmic membrane. As with other respiratory components, the organization and dynamics of this complex in living membranes is unknown. We set out to visualize the distribution and dynamics of this complex in vivo. By exchanging cydB for cydB-gfpgcn4 on the E. coli chromosome, we produced a strain (YTL01) that expresses functional GFP-tagged cytochrome bd-I terminal oxidase complexes under wild-type genetic control. We imaged live YTL01 cells using video-rate epifluorescence and total internal reflection fluorescence (TIRF) microscopy in combination with fluorescence recovery after photobleaching (FRAP) and saw mobile spots of GFP fluorescence in plasma membranes. Numbers of GFP molecules per spot were quantified by step-wise photobleaching giving a broad distribution with a mean of approximately 76, indicating that cytochrome bd-I is concentrated in mobile patches in the E. coli plasma membrane. We hypothesize that respiration occurs in mobile membrane patches which we call 'respirazones'
Cytochrome bd displays significant quinol peroxidase activity
No full textCytochrome bd is a prokaryotic terminal oxidase that catalyses the electrogenic reduction of oxygen to water using ubiquinol as electron donor. Cytochrome bd is a tri-haem integral membrane enzyme carrying a low-spin haem
Indigenous disparity in lower court imprisonment decisions: a study of two Australian jurisdictions, 1998 to 2008
No full textThis paper reports findings from statistical analyses of Indigeneity and lower court sentencing in New South Wales and South Australia from 1998 to 2008. The aim was to explore the probability of Indigenous versus non-Indigenous defendants receiving a prison sentence over time, while controlling for other key sentencing determinates (ie sex, age, criminal history, seriousness of current offence, plea, bail status). Across the study period, results generally showed that Indigenous offenders were more likely to receive a prison term than similarly situated non-Indigenous offenders.
However, the pattern of disparity over time differed by jurisdiction. In New South Wales, Indigenous offenders were more likely to receive a prison sentence throughout the entire period. By contrast, in the South Australian lower courts, disparity was found to have increased, with earlier years showing parity and leniency, before a trend towards a greater likelihood of a prison sentence for Indigenous offenders. Focal concerns theory is used to provide a possible explanation for the study’s finding of Indigenous lower court sentencing disparity
BD Rhapsody WTA AbSeq Analysis
No full text<p>Dataset and codes for BD Rhapsody single cell analysis system.</p>
<p>Disclaimer: The set of codes are optimised for the associated dataset only. For detailed understanding of the analysis procesure, please refer to https://github.com/INGEN-HOPE/Single-Cell-Multi-Omics-Data-Analysis-for-BD-Rhapsody. For any kind queries, please refer to the corresponding author, or post your query on our GitHub page.</p>
Lurasidone in children and adolescents: systematic review and case report
No full textObjective: To perform a systematic review of studies of lurasidone in children and/or adolescents and to present a case report aimed to add further insights into its use in clinical practice with youth.
Methods: We searched the following databases for empirical studies, of any design, focusing on the pharmacokinetics, efficacy, or safety of lurasidone in children and/or adolescents: Pubmed (Medline), OVID (PsycInfo, EMBASE+EMBASE classic, OVID Medline), Web of Knowledge, and ClinicalTrials.gov (last search January 23, 2018).
Results: From a pool of 301 potentially relevant references, we retained 12 pertinent studies (reported in 28 references), including 1 pharmacokinetics study, 1 double blind randomized controlled trial (RCT) for bipolar depression (BD) with 1 related interim analysis study of its extension phase and 1 related external posterior predictive check study, 1 double blind RCT for schizophrenia with 3 related interim analyses of its extension phase, 1 RCT and 1 case report for autism spectrum disorder, and 2 open-label studies focusing on a variety of disorders. Overall, these studies show that lurasidone is significantly more efficacious than placebo, with moderate effect sizes, and is well tolerated for BD and schizophrenia in youth. Published studies in youth have in general used doses up to 80 mg/day. Our case report suggests that high doses of lurasidone (148 mg/day) were well tolerated and might have contributed to substantial functional improvement in a 14-year old girl with psychosis and a previous history of anorexia nervosa, who had not responded to previous antipsychotics (olanzapine, risperidone, aripiprazole).
Conclusions: There is increasing evidence that lurasidone may be moderately effective and well tolerated for the treatment of BD and psychosis in youth and may have procognitive effects. Our case report suggests that future RCTs should assess the efficacy and tolerability of high doses (>80 mg/day) of lurasidone in youth
Growth of number of periodic orbits of one family of skew product maps
No full textIn this article we introduce a one-parameter family of skew product (Gt)t ∈ [−ε, ε] maps exhibiting a heterodimensional cycle such that the number of isolated periodic orbits inside it has not super-exponential growth. The dynamics in the central direction of the maps Gt is described by a one-parameter family of system of iterated functions.This work is part of the PhD thesis of the author, under the supervision
of Lorenzo Diaz and Jorge Rocha. The author thanks Professors Lorenzo Diaz and Jorge Rocha, for
having proposed this topic, by the stimulating conversations, the guidance and useful suggestions.
This research was funded by by the Portuguese government through the FCT (Fundação para a
Ciência e a Tecnologia) under the project PTDC/MAT/099493/2008. The author was supported by
the grants SFRH/BD/27674/2006 and SFRH/BD/49735/2009 of FCT.info:eu-repo/semantics/publishedVersio
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