101,930 research outputs found

    Allergeni modificati chimicamente e procedimento per la loro preparazione

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    Metodo per legare in modo stabile antigeni ed allergeni ad un supporto di polistirene consistenye nel far reagire un aldeide polifunzionale con la fase solida del polistirene; nel lavare detta fase solida per allontanare l'aldeide in eccesso; nell'incubare la fase solida così trattata con gli antigeni o gli allergeni, e nello stabilizzare il legame aldeide-antigeni o allergeni con sodio boro idruro o sodio ciano boroidruro

    Microbial transglutaminase: a new and emerging occupational allergen

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    Here we report a case of occupational sensitization to mTG in a company commercializing ingredients for the food industry. In the plant, raw materials are weighed and mixed in various finished goods

    Quantitative determination of Der p I allergen levels in allergenic extracts and house-dust samples

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    House-dust mites are responsible for serious respiratory diseases in humans. An ELISA assay to detect Dermatophagoides pteronyssinus (Dp) mites has been set up. This assay, based on quantitative determination of the major Dp allergen, called Der p I, has been applied for the analysis of growing mite cultures and house-dust samples. Der p I allergen levels in mite cultures are well correlated with the number of mites present as well as with the biological activity of the corresponding extracts. Different methods for detecting Dp mites in house-dust samples were compared. The ELISA method shows good sensitivity and specificity, and is particularly suitable for routine analyses

    Comparison of ammoniated and nonammoniated extracts in children with latex allergy

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    The use of ammoniated or nonammoniated latex extracts for the diagnosis of latex allergy is still a matter of debate. The aim of our study was to compare the characteristics of the two types of extracts by immunoblotting and RAST techniques in children with ascertained latex allergy
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