4,121 research outputs found

    How long can stool samples be fixed for an accurate diagnosis of soil-transmitted helminth infection using mini-FLOTAC?

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    Kato-Katz is a widely used method for the diagnosis of soil-transmitted helminth infection. Fecal samples cannot be preserved, and hence, should be processed on the day of collection and examined under a microscope within 60 min of slide preparation. Mini-FLOTAC is a technique that allows examining fixed fecal samples. We assessed the performance of Mini-FLOTAC using formalin-fixed stool samples compared to Kato-Katz and determined the dynamics of prevalence and intensity estimates of soil-transmitted helminth infection over a 31-day time period.; The study was carried out in late 2013 on Pemba Island, Tanzania. Forty-one children were enrolled and stool samples were subjected on the day of collection to a single Kato-Katz thick smear and Mini-FLOTAC examination; 12 aliquots of stool were fixed in 5% formalin and subsequently examined by Mini-FLOTAC up to 31 days after collection.; The combined results from Kato-Katz and Mini-FLOTAC revealed that 100% of children were positive for Trichuris trichiura, 85% for Ascaris lumbricoides, and 54% for hookworm. Kato-Katz and Mini-FLOTAC techniques found similar prevalence estimates for A. lumbricoides (85% versus 76%), T. trichiura (98% versus 100%), and hookworm (42% versus 51%). The mean eggs per gram of stool (EPG) according to Kato-Katz and Mini-FLOTAC was 12,075 and 11,679 for A. lumbricoides, 1,074 and 1,592 for T. trichiura, and 255 and 220 for hookworm, respectively. The mean EPG from day 1 to 31 of fixation was stable for A. lumbricoides and T. trichiura, but gradually declined for hookworm, starting at day 15. The findings of our study suggest that for a qualitative diagnosis of soil-transmitted helminth infection, stool samples can be fixed in 5% formalin for at least 30 days. However, for an accurate quantitative diagnosis of hookworm, we suggest a limit of 15 days of preservation. Our results have direct implication for integrating soil-transmitted helminthiasis into transmission assessment surveys for lymphatic filariasis

    Le tecniche chirurgiche mini-invasive.

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    Illustra le tecniche di chirurgia mini-invasiva dell'esofago

    Gli impianti mini e micro eolici

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    L’articolo, all’interno della diversificata gamma di possibilità di utilizzazione di energia da fonte rinnovabile, analizza sotto il profilo tecnico, economico e ambientale le diverse soluzioni oggi disponibili di impianti mini e micro eolici sia a scala urbana che per le utenze residenziali e per i settori dell’agricoltura, del turismo, della piccola e media impresa

    PER UNA EDIZIONE CRITICA DELLE RIME DI BARTOLOMEO CAVASSICO

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    This PhD thesis consists of: - An introductory part presenting the figure of the author, a description of the ms. bearing the Rime, an analysis of the form and content of the texts and the social, political and cultural context where they were produced; - The critical edition of fifty-four poems in italian and fifteen in Bellunese dialect. An introductory cap presents the formal characteristics of the work and provides the context, if necessary; this is followed by the text, reproduced according to generally conservative criteria; at the bottom there is a first band of apparatus, aimed at containing corrections of servile errors; a second band, containing the actual genetic apparatus, bearing any variants or previous redactions of the text; and finally the commentary, aimed at clarifying critical places and pointing out parallels with other authors. For the Bellunese texts, a full translation was also provided. - A caption index; - Two lexical indexes, for italian and Bellunese texts, bearing the notable forms; - Four appendices. Appendix I is a table containing all the poems, classified according to the following criteria: location in the ms., title, genre of the poem, language, possible addressee and brief summary, focusing, in particular, on the (recurring) themes present in the text. Appendix II houses vernacular texts not included in the edition, and Appendix III italian texts that the author has taken from other printings. Appendix IV contains the digital reproduction of six papers from ms. 396 of the Biblioteca Civica of Belluno.Questa tesi di dottorato si compone di: - Una parte introduttiva che presenta la figura dell'autore, la descrizione del ms. recante le Rime, l'analisi della forma e dei contenuti dei testi e il contesto sociale, politico e culturale in cui sono stati prodotti; - L'edizione critica commentata di cinquantaquattro poesie in volgare e quindici in dialetto bellunese. Un cappello introduttivo presenta le caratteristiche formali dell'opera e ne fornisce il contesto, qualora necessario; segue il testo, riprodotto secondo criteri generalmente conservativi; in calce si trovano una prima fascia di apparato, volta a contenere le correzioni degli errori servili, una seconda fascia, contenente l'apparato genetico vero e proprio, recante le eventuali varianti o redazioni precedenti del testo, e infine il commento, finalizzato a chiarire i luoghi critici e a segnalare paralleli con altri autori. Per i testi bellunesi si è fornita anche la traduzione integrale in calce. - Un indice dei capoversi; - Due indici lessicali, per i testi in volgare e per i testi in bellunese, recanti le forme notevoli; - Quattro appendici. L'appendice I è una tabella contenente tutti i componimenti, classificati secondo i seguenti criteri: collocazione nel ms., titolo, genere del componimento, lingua, eventuale destinatario e breve riassunto, imperniato, in particolare, sui temi (ricorrenti) presenti nel testo. L'appendice II ospita i testi in volgare non inclusi nell'edizione, mentre l'appendice III i testi in volgare che l'autore ha tratto da altre stampe. L'appendice IV contiene la riproduzione digitale di sei carte del ms. 396 della Biblioteca Civica di Belluno, attraverso la quale si possono osservare la scrittura del notaio e gli interventi sui testi

    Etnografia digitale, netnografia e autoetnografia: ricerche tra vecchi punk, giovani neopagani e membri di una vasta comunità BDSM della Repubblica ceca

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    Il capitolo presenta l'esperienza personale dell'autore, con l'utilizzo dei dati e delle esperienze raccolte attraverso internet, la netnografia come forma di ricerca qualitativa e l'accettazione personale dell'autoetnografia per la comprensione di sé, dell'altro e della cultura. Saranno analizzati 12 studi e ricerche realizzate dallo stesso autore, da solo o in qualità di coautore, tra gli anni 2005 e 2019, su temi di ricerca differenti, ma concentrati su selezionate sottoculture attive nella Repubblica Ceca. Il capitolo è concepito come un'introspezione diacronica in cui l'autore descrive i suoi primi tentativi di utilizzare pagine web e discussioni in internet come fonte di citazioni in articoli scientifici, fino al raggiungimento di una metodologia netnografica più matura.The chapter presents the personal experience of the author, with the use of data and experiences collected through the internet, the netnography as a form of qualitative research, and the personal acceptance of the autoethnography for the understanding of self, other, and culture. It will be analyzed 12 studies and researches realized by the same author, alone or as co-author, between the years 2005 and 2019, on different, but connected research topics concentred on selected sub-cultures active in the Czech Republic. The chapter is conceived as a diachronic introspection where the author describes his first attempts to use web pages and internet discussions as a source of quotations in scientific papers, up to the achievement of a more mature netnographic methodology

    Flotac and Mini-Flotac for uro-microscopic diagnosis of Capillaria plica (syn. Pearsonema plica) in dogs

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    Background: Capillaria plica (syn. Pearsonema plica) is a nematode that resides in the urinary bladder and rarely in ureters or in the kidney pelvis of various carnivores, especially foxes and dogs. Urine sedimentation technique is actually the only diagnostic tool that permits the identification of C. plica eggs, but its sensitivity is low and when an infection is suspected (or when it is necessary to confirm treatment efficacy) more than one examination of urine sediment should be performed. The present paper reports a clinical case of natural C. plica infection in a dog from southern Italy. In addition, two new techniques, FLOTAC and Mini-FLOTAC, were used for the diagnosis of C. plica in dog urine and compared with the technique of sedimentation. Results: Using FLOTAC with fresh urine and sodium chloride as flotation solution, were obtained the best results for the diagnosis of C. plica in dog urine in term of eggs counted (mean eggs per 10 ml of urine = 70.3 FLOTAC vs 40.3 Mini FLOTAC vs 32.8 sedimentation) and coefficient of variation (CV%) (6.2 FLOTAC vs 13.4 Mini-FLOTAC vs 32.9 sedimentation). Conclusions: The FLOTAC was the more sensitive method, but also the Mini-FLOTAC could be a valid alternative diagnostic method because gave better results than the classical sedimentation and can be used in place of the FLOTAC in laboratories where the centrifugation step cannot be performed. © 2014 Maurelli et al.; licensee BioMed Central Ltd

    L'inclusione dell'altro. Profilo di Giuseppe Nicolaci

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    The author retraces the teaching of Giuseppe Nicolaci and identifies in the inclusion of alterity his distinctive profile ad a man ans a teacher
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