18 research outputs found
sj-jpg-2-cll-10.1177_09636897231163232 – Supplemental material for Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization
No full textSupplemental material, sj-jpg-2-cll-10.1177_09636897231163232 for Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization by Michiko Shigyo, Yoshiomi Kobayashi, Oleksandr Platoshyn, Silvia Marsala, Tomohisa Kato Jr, Naoki Takamura, Kenji Yoshida, Akiyoshi Kishino, Mariana Bravo-Hernandez, Stefan Juhas, Jana Juhasova, Hana Studenovska, Vladimir Proks, Joseph D. Ciacci and Martin Marsala in Cell Transplantation</p
sj-jpg-3-cll-10.1177_09636897231163232 – Supplemental material for Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization
No full textSupplemental material, sj-jpg-3-cll-10.1177_09636897231163232 for Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization by Michiko Shigyo, Yoshiomi Kobayashi, Oleksandr Platoshyn, Silvia Marsala, Tomohisa Kato Jr, Naoki Takamura, Kenji Yoshida, Akiyoshi Kishino, Mariana Bravo-Hernandez, Stefan Juhas, Jana Juhasova, Hana Studenovska, Vladimir Proks, Joseph D. Ciacci and Martin Marsala in Cell Transplantation</p
sj-jpg-4-cll-10.1177_09636897231163232 – Supplemental material for Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization
No full textSupplemental material, sj-jpg-4-cll-10.1177_09636897231163232 for Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization by Michiko Shigyo, Yoshiomi Kobayashi, Oleksandr Platoshyn, Silvia Marsala, Tomohisa Kato Jr, Naoki Takamura, Kenji Yoshida, Akiyoshi Kishino, Mariana Bravo-Hernandez, Stefan Juhas, Jana Juhasova, Hana Studenovska, Vladimir Proks, Joseph D. Ciacci and Martin Marsala in Cell Transplantation</p
sj-jpg-1-cll-10.1177_09636897231163232 – Supplemental material for Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization
No full textSupplemental material, sj-jpg-1-cll-10.1177_09636897231163232 for Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization by Michiko Shigyo, Yoshiomi Kobayashi, Oleksandr Platoshyn, Silvia Marsala, Tomohisa Kato Jr, Naoki Takamura, Kenji Yoshida, Akiyoshi Kishino, Mariana Bravo-Hernandez, Stefan Juhas, Jana Juhasova, Hana Studenovska, Vladimir Proks, Joseph D. Ciacci and Martin Marsala in Cell Transplantation</p
Low-temperature fabrication of BaBi2Nb2O9 ceramics by reaction controlled sintering
No full textReaction controlled sintering was applied to the fabrication of BaBi2Nb2O9 (BBN) ceramics at lower temperature. A powder mixture of BaCO3 and Nb2O5 was heated at 600℃ in a 1st step calcination to produce a binary precursor of BaNb2O6. The pre-heated powder was then mixed with a fixed amount of Bi2O3, which was subsequently pressed into a disk pellet. After a powder compact of the mixture was subjected to heating at 950℃ for 4 h, a BBN bulk sample with a relative density of 92% was successfully obtained. The low-temperature fabrication of dense BBN ceramics could be attributed to the inhibited formation of an intermediate phase of Ba5Nb4O15 and the production of submicron powder with an appropriate reactivity during a 1st step calcination
Selective Formation of Porous Pt Nanorods for Highly Electrochemically Efficient Neural Electrode Interfaces
No full textThe enhanced electrochemical activity of nanostructured materials is readily exploited in energy devices, but their utility in scalable and human-compatible implantable neural interfaces can significantly advance the performance of clinical and research electrodes. We utilize low-temperature selective dealloying to develop scalable and biocompatible one-dimensional platinum nanorod (PtNR) arrays that exhibit superb electrochemical properties at various length scales, stability, and biocompatibility for high performance neurotechnologies. PtNR arrays record brain activity with cellular resolution from the cortical surfaces in birds and nonhuman primates. Significantly, strong modulation of surface recorded single unit activity by auditory stimuli is demonstrated in European Starling birds as well as the modulation of local field potentials in the visual cortex by light stimuli in a nonhuman primate and responses to electrical stimulation in mice. PtNRs record behaviorally and physiologically relevant neuronal dynamics from the surface of the brain with high spatiotemporal resolution, which paves the way for less invasive brain–machine interfaces
Ethylene oxidation activity of silica-supported platinum catalysts for the preservation of perishables
No full textThe ethylene oxidation activities of silica-supported platinum-based catalysts (Pt/A380 and PtRu/A380) were studied under semi-practical conditions (liter-sized batch system containing moisture and perishables). Storage tests of premature bananas, cucumbers, and apples have proved that the catalysts can remove ethylene generated from the perishables. Ripening of these perishables is significantly delayed by the catalytic removal of ethylene, which confirms that these catalysts are effective for the extension of the shelf-lives of the perishables. Two crucial parameters, the rate of ethylene production from the perishables and the rate of ethylene decomposition by the catalysts, were successfully quantified through storage tests of the perishables. The minimum amount of catalyst necessary for practical application in a storage room can be estimated by a simple numerical analysis using the determined parameters
New reliable scoring system, Toyama mouse score, to evaluate locomotor function following spinal cord injury in mice
No full textRoles of Cdc42 and Rac in Bergmann glia during cerebellar corticogenesis
No full textBergmann glia (BG) are important in the inward type of radial migration of cerebellar granule neurons (CGNs). However, details regarding the functions of Cdc42 and Rac in BG for radial migration of CGN are unknown. To examine the roles of Cdc42 and Rac in BG during cerebellar corticogenesis, mice with a single deletion of Cdc42 or Rac1 and those with double deletions of Cdc42 and Rac1 under control of the glial fibrillary acidic protein (GFAP) promoter: GFAP-Cre;Cdc42flox/flox (Cdc42-KO), GFAP–Cre;Rac1flox/flox (Rac1-KO), and GFAP-Cre; Cdc42 flox/flox;Rac1flox/flox (Cdc42/Rac1-DKO) mice, were generated. Both Cdc42-KO and Rac1-KO mice, but more obviously Cdc42-KO mice, had disturbed alignment of BG in the Purkinje cell layer (PCL). We found that Cdc42-KO, but not Rac1-KO, induced impaired radial migration of CGNs in the late phase of radial migration, leading to retention of CGNs in the lower half of the molecular layer (ML). Cdc42-KO, but not Rac1-KO, mice also showed aberrantly aligned Purkinje cells (PCs). These phenotypes were exacerbated in Cdc42/Rac1-DKO mice. Alignment of BG radial fibers in the ML and BG endfeet at the pial surface of the cerebellum evaluated by GFAP staining was disturbed and weak in Cdc42/Rac1-DKO mice, respectively. Our data indicate that Cdc42 and Rac, but predominantly Cdc42, in BG play important roles during the late phase of radial migration of CGNs. We also report here that Cdc42 is involved in gliophilic migration of CGNs, in contrast to Rac, which is more closely connected to regulating neurophilic migration
