621 research outputs found
Dr. Issac Clark, 1979
No full textDr. Issac Clark and a man exchange a handshake and a plaque award over a podium as others look on.The Atlanta University Center Robert W. Woodruff Library acknowledges the generous support of the National Endowment for Humanities - Humanities Collections and Reference Resources Implementation Project Grant in supporting the processing and digitization of a number of its major archival collections as part of the project: Spreading the Word: Expanding Access to African American Religious Archival Collections at the Atlanta University Center Robert W. Woodruff Library.</em
Programmed exosome fusion for energy generation in living cells
No full text© 2021, The Author(s), under exclusive licence to Springer Nature Limited.Biological membrane-enclosed organelles are fascinating examples of spatially confined nanoreactors for biocatalytic transformations such as cascade reactions involving multiple enzymes; however, the fabrication of their synthetic mimics remains a considerable challenge. Here we demonstrate supramolecular chemistry-based bridging of two membranes leading to controlled fusion of exosomes that act as nanoreactors for effective biocatalytic cascades, with prolonged functionality inside of living cells. Exosome membrane proteins were chemically engineered with a catechol moiety to drive fusion by supramolecular complexation to bridge the membranes. This strategy successfully encapsulated multiple enzymes and assembled the minimal electron transport chain in the plasma membrane, leading to tuneable, enhanced catalytic cascade activity capable of ATP synthesis inside of tissue spheroids. This nanoreactor was functional for many hours after uptake into living cells, showed successful penetration into tissue spheroids and repaired the damaged region by supplying ATP, all of which represent an advance in the mimicking of nature’s own organelles. [Figure not available: see fulltext.].11Nsciescopu
Cloaked Exosomes: Biocompatible, Durable, and Degradable Encapsulation
No full textExosomesnanosized extracellular vesicles (EVs) naturally secreted from cellshave emerged as promising biomarkers and potential therapeutic vehicles, but methods to manipulate them for engineering purposes remain elusive. Among the technical obstacles are the small size and surface complexity of exosomes and the complex processing steps required, which reduce the biocompatibility of currently available methods. The encapsulation of exosomes with a nanofilm of supramolecular complexes of ferric ions (Fe3+) and tannic acid is demonstrated here. The resulting natural polyphenol, approximate to 10 nm thick, protects exosomes from external aggressors such as UV-C irradiation or heat and is controllably degraded on demand. Furthermore, gold nanoparticles can be covalently attached for single-exosome level visualization. To fully exploit their therapeutic potential, chemotherapeutic drug-loaded EVs are functionalized to achieve the targeted, selective killing of cancer cells preferentially over normal cells. This nanofilm not only preserves the native size and chemical makeup of the intrinsic exosomes, but also confers new capabilities for efficient tumor targeting and pH-controlled release of drugs. Demonstrating a scalable method to produce biocompatible, durable, on-demand degradable, and chemically controllable shields for exosome modification and functionalization, the methods introduced here are expected to bring the potential of exosome-based nanomedicine applications closer to reality
A fidget spinner for the point-of-care diagnosis of urinary tract infection
No full text© 2020, The Author(s), under exclusive licence to Springer Nature Limited.The point-of-care detection of pathogens in biological samples in resource-limited settings should be inexpensive, rapid, portable, simple and accurate. Here, we describe a custom-made fidget spinner that rapidly concentrates pathogens in 1-ml samples of undiluted urine by more than 100-fold for the on-device colorimetric detection of bacterial load and pathogen identification. In Tiruchirappalli, India, the device enabled the on-site detection of infection with the naked eye within 50 min in urine samples from 39 patients suspected of having a urinary tract infection. We also show that, in 30 clinical samples of urinary tract infection, the device can be used to perform an antimicrobial susceptibility test for the antimicrobial drugs ciprofloxacin and cefazolin within 120 min. The fidget spinner could be used in low-resource settings as an inexpensive handheld point-of-care device for the rapid concentration and detection of pathogens in urine sample
Surface-Engineered Paper Hanging Drop Chip for 3D Spheroid Culture and Analysis
No full textProtein corona coated onto the hydrophilic cellulose fiber turns into hydrophobic upon UV irradiation without hindering the porosity of the paper while simultaneously reducing nonspecific adsorption. Taking advantage of the biofouling-resistant, hydrophobic, and fluid transport through property, we demonstrated hanging drop three-dimensional (3D) spheroid culture and in-site analysis, including drug testing, time-dependent detection of secreted protein, and fluorescence staining without disturbing the spheroids. This single hanging drop system can also be extended to a networked hanging drop chip to mimic in vivo microphysiology by combining with wax-patterned microfluidic channels, where well-to-well interaction can be accurately controlled in a passive manner. As a proof of concept, the effects of a concentration gradient of nutrient and variable dosage of anticancer drugs were studied in the 3D spheroids cultured on paper. The experimental results suggested that a complex network device could be fabricated on a large scale on demand at a minimal cost for 3D spheroid culture. Our method demonstrates a future possibility for paper as a low cost, high-throughput 3D spheroid-based ???body-on-a-chip??? platform material
Editorial Team
No full textThe Toro Historical Review Volume 15, Issue 2
Editorial and Production Team
Michael Gonzalez
Ava Kostka
Issac Rivera
Dr. Joshua J. Jeffers, faculty mento
Austin Papers: Series II, Part I, 1794-1817
No full textCopy of transcript for a letter addressed to Issac Tomlinson and Co. or Issac Tomlinson and Sons, in which the author explains taking out loans in order to transport goods
A pastoral leadership model of mutuality for greater true vine Baptist church, 2016
No full textThe purpose of this project is to promote transformative change within a local church by establishing an ethos of mutuality built upon mutually shared religious beliefs rather than shared culture or age. The pastor of a predominantly African-American church of approximately two hundred members located in the City of Pensacola in the panhandle of Florida observed that there was no perceived cooperative and collaborative exchange between those who shared a culture (individuals bound together with certain beliefs, interests, and culture) or those who were from the same generation within the church. The church being studied is an urban inner-city ministry comprised of 71 % adults and 29% youths. Culturally, the differences among members are more geographical in their origin than racial. Pensacola, a city with a fluid population, attracts people from different communities within the nation. Utilizing the concepts of task competence, transactional commitment, and transformative consciousness (consistent mental awareness of transformative praxis) for the pastor and the membership, this project addresses transformative change (ministry involvement, organizational commitment, and membership retention) as well as attitudes, behaviors, and actions within the local church. The project includes the development of a four-lesson series that addresses the idea of mutuality (embracing shared core beliefs and ideas). The project enrolled twenty-one people representing each ministry subgroup within the church. The group completed a pre-test and post-test (questionnaire) and participated in all group session activities designed to develop mutuality in the areas of core shared beliefs such as spiritual growth, and the church's direction of ministry, vision, and mission. The effectiveness of the lesson plan series was evaluated based on increased unity, behavioral attitudes towards each other, and praxis in the ministry. The community known as the Greater True Vine Missionary Baptist Church of Pensacola, Florida is the project's primary focus. The hoped for outcome of the project was to create greater unity and organizational commitment among the church's members as a result of their renewed focus on their shared core religious beliefs
The movement op thought in Egypt under the middle kingdom and the empire and in china from the Han dynasty to the Ming dynasty A comparative study, 1956
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